| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | Exported | 2446401 = | 64 (1.080) | 4 (0.080) | 3/90 | NT | 7.1% | coding (12/264 nt) | ilvM | acetolactate synthase II, small subunit |
| ? | Exported | 2446430 = | 49 (0.940) | pseudogene (1626/1647 nt) | ilvG | Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II | |||||
GAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < Exported/2446474‑2446401‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caacatTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAG > Exported/2446430‑2446498 GAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGC < 1:327117/69‑1GAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGC < 1:465296/69‑1 AGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAAC < 1:1690657/71‑1 AACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACAT > 1:3432495/1‑71 CGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACAT > 1:2835271/1‑69 CGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACAT > 1:34030/1‑69 GTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAA < 1:2218731/43‑1 TGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCA < 1:859157/41‑1 AGAAATGTTGGAGAAATTATCATGATGCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCG < 1:158549/65‑1 AGAAATGTTGGAGAAATTATCATGATGCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCG < 1:1738510/65‑1 AAATGTTGGAGAAATTATCATGATGCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCATAC > 1:1471495/1‑70 AATGTTGGAGAAATTATCATGATGCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACG > 1:49675/1‑70 CAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTG > 1:2738607/1‑65 AACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCA < 1:2736295/51‑1 ACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATG > 1:3074271/1‑70 ATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAG > 1:2809274/1‑71 GAGAACGTCTGGCCGCTGGTGCCGCCTGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGATGCAACAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < Exported/2446474‑2446401‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caacatTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAG > Exported/2446430‑2446498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |