Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 863,793 G→T 14.0% E32* (GAA→TAA)  yciQ → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported863,7930GT14.0% 150.3 / 8.9 50E32* (GAA→TAA) yciQpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base T (4/3);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

TTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAA  >  Exported/863724‑863862
                                                                      |                                                                     
ttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATg                                                                       <  1:906306/70‑1 (MQ=255)
ttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATg                                                                       <  1:3208089/70‑1 (MQ=255)
   cTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaaga                                                                   <  1:1704669/71‑1 (MQ=255)
   cTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaag                                                                    <  1:2672846/70‑1 (MQ=255)
   cTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaag                                                                    <  1:206318/70‑1 (MQ=255)
   cTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaag                                                                    <  1:1658272/70‑1 (MQ=255)
      ttGATAGCAGGGCTGTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAgg                                                                <  1:274555/70‑1 (MQ=255)
        gATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAggg                                                               <  1:1510241/70‑1 (MQ=255)
        gATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGa                                                              >  1:1151319/1‑71 (MQ=255)
              aGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGc                                                        >  1:981965/1‑71 (MQ=255)
              aGGGCTGTTTGTATCATCTCTAAGTTACGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGc                                                        >  1:68991/1‑71 (MQ=255)
               gggCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGc                                                        >  1:2622985/1‑70 (MQ=255)
                     tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATaagaag                                                                 <  1:1533414/55‑1 (MQ=37)
                     tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATaagaag                                                                 <  1:63038/55‑1 (MQ=37)
                     tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATaagaag                                                                 <  1:94091/55‑1 (MQ=37)
                     tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCttt                                                   <  1:1239832/69‑1 (MQ=255)
                       tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATAAGAAggg                                                               >  1:2685518/1‑55 (MQ=25)
                       tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGaagaag                                                                 >  1:1158005/1‑53 (MQ=255)
                       tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATg                                               >  1:3652887/1‑71 (MQ=255)
                       tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATg                                               >  1:3725011/1‑71 (MQ=255)
                           tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCATAAGAAgg                                                                >  1:1453542/1‑50 (MQ=25)
                           tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTg                                            <  1:3172613/70‑1 (MQ=255)
                           tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTg                                            <  1:3011424/70‑1 (MQ=255)
                           tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTg                                            <  1:1254451/70‑1 (MQ=255)
                           tcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa                                           <  1:1534084/71‑1 (MQ=255)
                             atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGa                                                              <  1:1063348/50‑1 (MQ=255)
                             atcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGc                                                        >  1:2076942/1‑56 (MQ=255)
                                tctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCa                                      >  1:1435742/1‑71 (MQ=255)
                                   aaGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCActc                                   >  1:1966979/1‑71 (MQ=255)
                                   aaGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCActc                                   >  1:1687068/1‑71 (MQ=255)
                                    aGTTATGCAGAAAACACGGAGATCCCTTCTTATgaa                                                                     >  1:2963731/1‑36 (MQ=255)
                                    aGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCAct                                    <  1:2537342/69‑1 (MQ=255)
                                     gTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg                                 <  1:1871918/71‑1 (MQ=255)
                                     gTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg                                 <  1:955882/71‑1 (MQ=255)
                                      ttATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGc                                <  1:24186/71‑1 (MQ=255)
                                        aTGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa                                           >  1:2490978/1‑58 (MQ=255)
                                        aTGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGa                                           >  1:750182/1‑58 (MQ=255)
                                               aaaCACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGAt                        <  1:2855137/70‑1 (MQ=255)
                                               aaaCACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATg                       <  1:2327904/71‑1 (MQ=255)
                                               aaaCACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATg                       >  1:1223584/1‑71 (MQ=255)
                                                  cacGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgggg                    <  1:2454551/71‑1 (MQ=255)
                                                    cGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg                                 >  1:2795908/1‑56 (MQ=255)
                                                    cGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTg                  >  1:839341/1‑71 (MQ=255)
                                                      gagaTCCCTTCTTATGAATAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg                                 <  1:3678386/54‑1 (MQ=255)
                                                         aTCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGa             >  1:1588448/1‑71 (MQ=255)
                                                           cccTTCTTCATGAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACa           >  1:3352957/1‑71 (MQ=38)
                                                           cccTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCaa                              >  1:865887/1‑52 (MQ=25)
                                                           cccTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCaa                              >  1:2137300/1‑52 (MQ=25)
                                                           cccTTCTTATGAAGAAGGGATCTCGCTCTTTGATg                                               <  1:3712868/35‑1 (MQ=255)
                                                              ttcttATGAAGAAGGGATCTCGCTCTTTGATGTTGaa                                          <  1:3412391/37‑1 (MQ=255)
                                                              ttcttATGAAGAAGGGATCTCGCTCTTTGATGTTGaa                                          <  1:3028768/37‑1 (MQ=255)
                                                              ttcttATGAAGAAGGGATCTCGCTCTTTGATGTTGaa                                          <  1:2655560/37‑1 (MQ=255)
                                                              ttcttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATc         >  1:164709/1‑70 (MQ=255)
                                                                  tATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAg     >  1:2284058/1‑70 (MQ=255)
                                                                    aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAAc                             <  1:231805/45‑1 (MQ=255)
                                                                     tGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGaaa  <  1:380802/71‑1 (MQ=255)
                                                                     tGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACAAGATGGGGTGCTCGACATCAAAGaa   >  1:928469/1‑70 (MQ=255)
                                                                      |                                                                     
TTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAA  >  Exported/863724‑863862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: