Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 922,265 C→G 6.3% A112G (GCA→GGA)  ycjZ → predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported922,2650CG 6.3% 355.8 / 9.2 111A112G (GCA→GGA) ycjZpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (49/55);  new base G (5/2);  total (54/57)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 113 total aligned reads displayed.

GAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGAT  >  Exported/922197‑922335
                                                                    |                                                                      
gAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCaa                                                                      <  1:2375129/71‑1 (MQ=255)
  aCCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAAt                                                                     <  1:2057911/70‑1 (MQ=255)
  aCCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATg                                                                    <  1:1279738/71‑1 (MQ=255)
         aTCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACAt                                                             <  1:691423/71‑1 (MQ=255)
         aTCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACAt                                                             <  1:1758105/71‑1 (MQ=255)
         aTCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACAt                                                             <  1:1452589/71‑1 (MQ=255)
            cGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTc                                                           <  1:447005/70‑1 (MQ=255)
             gggACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCt                                                         >  1:2087730/1‑71 (MQ=255)
               gACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa                                                       <  1:453697/71‑1 (MQ=255)
                aCAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa                                                      <  1:2192937/71‑1 (MQ=255)
                aCAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa                                                      <  1:3106836/71‑1 (MQ=255)
                 cAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa                                                      <  1:1639679/70‑1 (MQ=255)
                  aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGca                                                                <  1:743191/59‑1 (MQ=255)
                  aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGca                                                                <  1:2502288/59‑1 (MQ=255)
                  aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGca                                                                <  1:551618/59‑1 (MQ=255)
                  aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa                                                       <  1:1374955/68‑1 (MQ=255)
                  aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa                                                       <  1:3730095/68‑1 (MQ=255)
                   aTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATc                                                   <  1:2092342/71‑1 (MQ=255)
                    tACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg                                                  >  1:441132/1‑71 (MQ=255)
                     aCGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg                                                  >  1:1453972/1‑70 (MQ=255)
                      cGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc                                                 <  1:3407616/70‑1 (MQ=255)
                         aTTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATcc                                             >  1:2447484/1‑71 (MQ=255)
                           tactacTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCt                                            >  1:3510836/1‑70 (MQ=255)
                            actactGTAGAACATGCAGCAAAAACGATATTGTTACCAGc                                                                        <  1:1462338/41‑1 (MQ=255)
                             ctactGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcgc                                                                 >  1:2213012/1‑47 (MQ=255)
                             ctactGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcgc                                                                 >  1:938001/1‑47 (MQ=255)
                             ctactGTAGAACATGCAGAAAAAACGATATTGTTACCAGCAATgcgc                                                                 >  1:630727/1‑47 (MQ=255)
                              tactGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGaaa                                        >  1:1841707/1‑71 (MQ=255)
                              tactGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGaaa                                        >  1:687780/1‑71 (MQ=255)
                                ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcg                                                                  <  1:1151844/43‑1 (MQ=255)
                                ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcg                                                                  <  1:2965682/43‑1 (MQ=255)
                                ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcg                                                                  <  1:1825377/43‑1 (MQ=255)
                                ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATgcg                                                                  <  1:2151044/43‑1 (MQ=255)
                                ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAAtt                                      <  1:1564618/71‑1 (MQ=255)
                                 tGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATcc                                             >  1:3123399/1‑63 (MQ=255)
                                   tAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTg                                     <  1:503395/69‑1 (MQ=255)
                                   tAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTg                                     <  1:3134036/69‑1 (MQ=255)
                                     gAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGatat                                 <  1:2154915/71‑1 (MQ=255)
                                       aCATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                >  1:3275693/1‑70 (MQ=255)
                                       aCATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTc                               <  1:107743/71‑1 (MQ=255)
                                        cATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                >  1:664588/1‑69 (MQ=255)
                                        cATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                >  1:125519/1‑69 (MQ=255)
                                        cATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                >  1:3429723/1‑69 (MQ=255)
                                          tGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGa                                          >  1:210501/1‑57 (MQ=255)
                                          tGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGa                                          >  1:1936703/1‑57 (MQ=255)
                                          tGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGa                                          >  1:2233808/1‑57 (MQ=255)
                                           gcagcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGata                                  <  1:2882308/64‑1 (MQ=255)
                                             agcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                >  1:1515213/1‑64 (MQ=255)
                                             agcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                >  1:1156599/1‑64 (MQ=255)
                                               caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTccc                        >  1:2982144/1‑68 (MQ=255)
                                               caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCAc                        >  1:3585991/1‑70 (MQ=255)
                                               caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCAc                        >  1:3011612/1‑70 (MQ=255)
                                               caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCAc                        >  1:1810130/1‑70 (MQ=255)
                                                aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCt                           <  1:2083418/66‑1 (MQ=255)
                                                aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa                      <  1:430360/71‑1 (MQ=255)
                                                aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa                      >  1:2068546/1‑71 (MQ=255)
                                                 aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg                                                  >  1:3159153/1‑42 (MQ=255)
                                                 aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc                                                 >  1:1613448/1‑43 (MQ=255)
                                                 aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc                                                 >  1:2488980/1‑43 (MQ=255)
                                                 aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc                                                 >  1:2009630/1‑43 (MQ=255)
                                                 aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGaaa                                        >  1:1921845/1‑52 (MQ=255)
                                                  aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                    >  1:1305410/1‑71 (MQ=255)
                                                  aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                    >  1:111846/1‑71 (MQ=255)
                                                  aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                    >  1:751763/1‑71 (MQ=255)
                                                  aaaCGATATTGTTACAAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                    >  1:3634763/1‑71 (MQ=255)
                                                   aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAg                             >  1:1490914/1‑61 (MQ=255)
                                                   aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                    >  1:3555214/1‑70 (MQ=255)
                                                   aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg                   <  1:1987888/71‑1 (MQ=255)
                                                   aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg                   <  1:2344720/71‑1 (MQ=255)
                                                   aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg                   <  1:83746/71‑1 (MQ=255)
                                                      gATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAtt                <  1:2140374/71‑1 (MQ=255)
                                                      gATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAtt                <  1:2413683/71‑1 (MQ=255)
                                                       atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa               <  1:3198011/71‑1 (MQ=255)
                                                       atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa               <  1:1625759/71‑1 (MQ=255)
                                                       atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa               <  1:409118/71‑1 (MQ=255)
                                                        tatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTAt              >  1:1415333/1‑71 (MQ=255)
                                                           tGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                    <  1:269295/62‑1 (MQ=255)
                                                           tGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATgg            <  1:107499/70‑1 (MQ=255)
                                                            gTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt          >  1:3347952/1‑71 (MQ=255)
                                                             ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt         <  1:2172813/71‑1 (MQ=255)
                                                             ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt         <  1:1827514/71‑1 (MQ=255)
                                                             ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt         <  1:2433355/71‑1 (MQ=255)
                                                             ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt          <  1:1577755/70‑1 (MQ=255)
                                                               ttcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGa       >  1:3327062/3‑71 (MQ=37)
                                                                tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAAtt                                      <  1:863317/38‑1 (MQ=14)
                                                                tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCAcc                       >  1:485743/2‑54 (MQ=25)
                                                                tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAc      >  1:1650955/2‑71 (MQ=38)
                                                                tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAc      >  1:698845/2‑71 (MQ=38)
                                                                ccAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGa       <  1:1262701/70‑1 (MQ=255)
                                                                 cAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc     >  1:675531/1‑71 (MQ=255)
                                                                 cAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc     >  1:2710616/1‑71 (MQ=255)
                                                                 cAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc     >  1:616314/1‑71 (MQ=255)
                                                                  aGCAATGCGCACATTCCTGAAATCGCATCCTGaaa                                        <  1:139078/35‑1 (MQ=255)
                                                                  aGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCg    <  1:663895/71‑1 (MQ=255)
                                                                   gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc     >  1:2882305/1‑69 (MQ=255)
                                                                   gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc     >  1:796053/1‑69 (MQ=255)
                                                                   gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc     >  1:2675162/1‑69 (MQ=255)
                                                                   gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCg    <  1:2913256/70‑1 (MQ=255)
                                                                   gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGa   >  1:780398/1‑71 (MQ=255)
                                                                    cAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGAt  >  1:189216/1‑71 (MQ=255)
                                                                    |                                                                      
GAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGAT  >  Exported/922197‑922335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: