Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 922,278 C→G 7.6% F116L (TTC→TTG ycjZ → predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported922,2780CG 7.6% 372.6 / 13.1 118F116L (TTC→TTGycjZpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (60/49);  new base G (5/4);  total (65/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAA  >  Exported/922209‑922347
                                                                     |                                                                     
cGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTc                                                                       <  1:447005/70‑1 (MQ=255)
 gggACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCt                                                                     >  1:2087730/1‑71 (MQ=255)
   gACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa                                                                   <  1:453697/71‑1 (MQ=255)
    aCAATACGTATTACTACTGTAGAACATGCAGCAAAATCGATATTGTTACCAGCAATGCGCACATTCCTGa                                                                   <  1:3093094/70‑1 (MQ=255)
    aCAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa                                                                  <  1:2192937/71‑1 (MQ=255)
    aCAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa                                                                  <  1:3106836/71‑1 (MQ=255)
     cAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGaa                                                                  <  1:1639679/70‑1 (MQ=255)
      aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa                                                                   <  1:3730095/68‑1 (MQ=255)
      aaTACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGa                                                                   <  1:1374955/68‑1 (MQ=255)
        tACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg                                                              >  1:441132/1‑71 (MQ=255)
         aCGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg                                                              >  1:1453972/1‑70 (MQ=255)
             aTTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATcc                                                         >  1:2447484/1‑71 (MQ=255)
               tactacTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCt                                                        >  1:3510836/1‑70 (MQ=255)
                  tactGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGaaa                                                    >  1:687780/1‑71 (MQ=255)
                    ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAAtt                                                  <  1:1564618/71‑1 (MQ=255)
                    ctGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAAtt                                                  <  1:577180/71‑1 (MQ=255)
                     tGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATcc                                                         >  1:3123399/1‑63 (MQ=255)
                       tAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTg                                                 <  1:3134036/69‑1 (MQ=255)
                       tAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTg                                                 <  1:503395/69‑1 (MQ=255)
                         gAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGatat                                             <  1:2154915/71‑1 (MQ=255)
                           aCATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                            >  1:2356926/1‑70 (MQ=255)
                           aCATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                            >  1:3275693/1‑70 (MQ=255)
                           aCATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTc                                           <  1:107743/71‑1 (MQ=255)
                            cATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                            >  1:3429723/1‑69 (MQ=255)
                            cATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                            >  1:664588/1‑69 (MQ=255)
                            cATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                            >  1:125519/1‑69 (MQ=255)
                              tGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGa                                                      >  1:1936703/1‑57 (MQ=255)
                              tGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGa                                                      >  1:210501/1‑57 (MQ=255)
                              tGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGa                                                      >  1:2233808/1‑57 (MQ=255)
                               gcagcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGata                                              <  1:2882308/64‑1 (MQ=255)
                                 agcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                            >  1:1156599/1‑64 (MQ=255)
                                  gcaAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTg                                                 <  1:1505261/58‑1 (MQ=255)
                                   caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTccc                                    >  1:2982144/1‑68 (MQ=255)
                                   caAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCAc                                    >  1:1810130/1‑70 (MQ=255)
                                    aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCt                                       <  1:2083418/66‑1 (MQ=255)
                                    aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa                                  >  1:2068546/1‑71 (MQ=255)
                                    aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa                                  <  1:430360/71‑1 (MQ=255)
                                    aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa                                  <  1:2131236/71‑1 (MQ=255)
                                    aaaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCa                                  >  1:2687009/1‑71 (MQ=255)
                                     aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCg                                                              >  1:3159153/1‑42 (MQ=255)
                                     aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc                                                             >  1:1613448/1‑43 (MQ=255)
                                     aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGc                                                             >  1:2488980/1‑43 (MQ=255)
                                     aaaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGaaa                                                    >  1:1921845/1‑52 (MQ=255)
                                      aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                                >  1:751763/1‑71 (MQ=255)
                                      aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                                >  1:1305410/1‑71 (MQ=255)
                                      aaaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                                >  1:111846/1‑71 (MQ=255)
                                      aaaCGATATTGTTACAAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                                >  1:3634763/1‑71 (MQ=255)
                                       aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAg                                         >  1:1490914/1‑61 (MQ=255)
                                       aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                                >  1:3555214/1‑70 (MQ=255)
                                       aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg                               <  1:2344720/71‑1 (MQ=255)
                                       aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg                               <  1:83746/71‑1 (MQ=255)
                                       aaCGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTg                               <  1:1987888/71‑1 (MQ=255)
                                          gATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAtt                            <  1:2140374/71‑1 (MQ=255)
                                          gATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAtt                            <  1:2413683/71‑1 (MQ=255)
                                           atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa                           <  1:1980024/71‑1 (MQ=255)
                                           atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa                           <  1:3198011/71‑1 (MQ=255)
                                           atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa                           <  1:1301058/71‑1 (MQ=255)
                                           atatTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTa                           <  1:1625759/71‑1 (MQ=255)
                                               tGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                                <  1:269295/62‑1 (MQ=255)
                                               tGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCAtt                                <  1:1766315/62‑1 (MQ=255)
                                               tGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATgg                        <  1:107499/70‑1 (MQ=255)
                                                gTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt                      >  1:3347952/1‑71 (MQ=255)
                                                gTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt                      >  1:3402808/1‑71 (MQ=255)
                                                 ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt                     <  1:1827514/71‑1 (MQ=255)
                                                 ttACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGtt                      <  1:1577755/70‑1 (MQ=255)
                                                   ttcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGa                   >  1:3327062/3‑71 (MQ=37)
                                                    tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAAtt                                                  <  1:3731231/38‑1 (MQ=14)
                                                    tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAAtt                                                  <  1:863317/38‑1 (MQ=14)
                                                    tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAc                  >  1:732965/2‑71 (MQ=38)
                                                    tcAGGAATGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAc                  >  1:698845/2‑71 (MQ=38)
                                                     cAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc                 >  1:616314/1‑71 (MQ=255)
                                                      aggaaTGTGCGCATTGCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc                 <  1:3541301/67‑1 (MQ=255)
                                                      aGCAATGCGCACATTCCTGAAATCGCATCCTGaaa                                                    <  1:139078/35‑1 (MQ=255)
                                                      aGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCg                <  1:663895/71‑1 (MQ=255)
                                                       gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc                 >  1:796053/1‑69 (MQ=255)
                                                       gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc                 >  1:2675162/1‑69 (MQ=255)
                                                       gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGAcc                 >  1:2882305/1‑69 (MQ=255)
                                                       gCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCg                <  1:2913256/70‑1 (MQ=255)
                                                        gaaTGTGCGCATTGCTGAAATCGCATCCTGAAAtt                                                  <  1:2847366/34‑1 (MQ=18)
                                                        cAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGAt              >  1:189216/1‑71 (MQ=255)
                                                        aaaTGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGAt              >  1:1458206/2‑71 (MQ=255)
                                                           tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTTATTATGGTTTGACCGATGTc           >  1:329376/1‑71 (MQ=255)
                                                           tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAt                             >  1:735073/1‑53 (MQ=255)
                                                           tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAt                             >  1:1526551/1‑53 (MQ=255)
                                                           tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGAt                             >  1:2110042/1‑53 (MQ=255)
                                                           tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTc           <  1:1322330/71‑1 (MQ=255)
                                                           tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTc           <  1:785327/71‑1 (MQ=255)
                                                           tGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTc           >  1:2091098/1‑71 (MQ=255)
                                                             cgcACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGt         >  1:3627474/1‑71 (MQ=255)
                                                              gcACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGtt        <  1:3704649/71‑1 (MQ=255)
                                                              gcACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGtt        <  1:1217657/71‑1 (MQ=255)
                                                               cacaTTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGttt       >  1:2091369/1‑71 (MQ=255)
                                                               cacaTTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGttt       <  1:176142/71‑1 (MQ=255)
                                                               cacaTTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGttt       <  1:1748193/71‑1 (MQ=255)
                                                               cacaTTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGttt       <  1:345773/71‑1 (MQ=255)
                                                                 caTTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATgg                        >  1:2126177/1‑52 (MQ=255)
                                                                 caTTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATgg                        >  1:868680/1‑52 (MQ=255)
                                                                   ttCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTc      >  1:1497745/1‑68 (MQ=255)
                                                                   ttCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTc      >  1:439330/1‑68 (MQ=255)
                                                                    tCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGaa  >  1:485686/1‑71 (MQ=255)
                                                                     |                                                                     
CGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAA  >  Exported/922209‑922347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: