| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 939,576 | C→A | 7.6% | E399D (GAG→GAT) | sfcA ← | malate dehydrogenase, NAD‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 939,576 | 0 | C | A | 7.6% | 352.4 / 8.2 | 104 | E399D (GAG→GAT) | sfcA | malate dehydrogenase, NAD‑requiring |
| Reads supporting (aligned to +/- strand): ref base C (52/44); new base A (5/3); total (57/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
GTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACAT > Exported/939509‑939646 | gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:3270018/71‑1 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:2516482/71‑1 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:1806356/71‑1 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct > 1:1752243/1‑71 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct > 1:3402552/1‑71 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:1658478/71‑1 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:1614655/71‑1 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct > 1:3125106/1‑71 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct > 1:85636/1‑71 (MQ=255) gACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTc > 1:3145206/1‑71 (MQ=255) aCGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTc < 1:2894225/70‑1 (MQ=255) cGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCg < 1:3053179/71‑1 (MQ=255) cGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCg > 1:2270259/1‑71 (MQ=255) gTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt < 1:436905/71‑1 (MQ=255) tCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTcc < 1:3044415/68‑1 (MQ=255) tCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTa < 1:1907867/71‑1 (MQ=255) gggTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGt > 1:3501467/1‑68 (MQ=255) tAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGc > 1:1569689/1‑71 (MQ=255) aGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGcc > 1:3199670/1‑71 (MQ=255) aGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGcc > 1:3195252/1‑71 (MQ=255) aCAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCg > 1:558845/1‑71 (MQ=255) aCAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCg < 1:2310588/71‑1 (MQ=255) cAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGc > 1:719520/1‑67 (MQ=255) aGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:2024210/54‑1 (MQ=255) aGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt < 1:1030223/71‑1 (MQ=255) aGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt > 1:3645963/1‑71 (MQ=255) aGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt < 1:1239196/71‑1 (MQ=255) cGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCt < 1:3410231/71‑1 (MQ=255) ggCATCTCGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt > 1:1668960/1‑56 (MQ=25) ggCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTg < 1:891171/71‑1 (MQ=255) ggCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt > 1:3226233/1‑56 (MQ=37) ggCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt > 1:107888/1‑56 (MQ=37) ggCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt > 1:3112825/1‑56 (MQ=37) cATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTc > 1:2123070/1‑71 (MQ=255) cATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTc > 1:2198769/1‑71 (MQ=255) tCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGcc < 1:102467/60‑1 (MQ=255) tCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGcc < 1:1737588/60‑1 (MQ=255) aCGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTa < 1:532188/51‑1 (MQ=255) aCGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga < 1:2058904/71‑1 (MQ=255) aCGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga < 1:2389310/71‑1 (MQ=255) aCGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga < 1:1928104/71‑1 (MQ=255) aCGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga < 1:438895/71‑1 (MQ=255) gATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCg < 1:2060658/47‑1 (MQ=255) gATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTaaa > 1:2133578/1‑51 (MQ=255) tCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTTAGACg > 1:452566/1‑71 (MQ=255) tCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACg < 1:42438/71‑1 (MQ=255) cGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGc > 1:3523060/1‑71 (MQ=255) cGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGc > 1:1223913/1‑71 (MQ=255) aCGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTaaa > 1:731882/1‑45 (MQ=255) aCGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCaa < 1:3380250/71‑1 (MQ=255) aCGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCa < 1:2680453/70‑1 (MQ=255) aCGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt < 1:1902080/42‑1 (MQ=21) cgcgGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAAt > 1:2793243/1‑71 (MQ=255) cgcgGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAAt > 1:3393025/1‑71 (MQ=255) cgcgGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAAt > 1:1879218/1‑71 (MQ=255) gcgGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCaa > 1:2808786/1‑69 (MQ=255) gcgGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAAt > 1:2679521/1‑70 (MQ=255) aCAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAAc > 1:2397389/1‑40 (MQ=255) aCAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGaa > 1:2933318/1‑71 (MQ=255) aCAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGaa > 1:2660339/1‑71 (MQ=255) aGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAata > 1:1932948/1‑71 (MQ=255) gtgtTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTg < 1:2118045/53‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCt < 1:3424025/44‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTg < 1:2823097/45‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTg < 1:26887/45‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCt < 1:443004/70‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTg > 1:995438/1‑71 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTg > 1:2610544/1‑71 (MQ=255) ttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTgag < 1:394954/51‑1 (MQ=255) tATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGt > 1:84488/1‑71 (MQ=255) tATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGt > 1:620140/1‑71 (MQ=255) tATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGt > 1:3246355/1‑71 (MQ=255) tGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGttt < 1:1582098/71‑1 (MQ=255) tGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGttt < 1:2503321/71‑1 (MQ=255) tGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGtt < 1:3545968/70‑1 (MQ=255) cATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt > 1:1899240/1‑36 (MQ=255) cATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt > 1:3418191/1‑36 (MQ=255) cATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt > 1:460512/1‑36 (MQ=255) cATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga > 1:2406597/1‑45 (MQ=255) cATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga > 1:1076166/1‑45 (MQ=255) attctcACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTa < 1:2511526/69‑1 (MQ=255) aTCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTa < 1:2438811/70‑1 (MQ=255) tctcACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGaa < 1:1115718/57‑1 (MQ=255) ctcACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACAt > 1:3221829/1‑71 (MQ=255) cACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTg > 1:1920471/1‑71 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACa < 1:1870295/67‑1 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACAt < 1:1773872/68‑1 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACAt < 1:3646009/68‑1 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgc > 1:2747206/1‑71 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgc > 1:2794910/1‑71 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgc > 1:803884/1‑71 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgc > 1:1549046/1‑71 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgc > 1:2554372/1‑71 (MQ=255) cGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgcg > 1:2566834/1‑71 (MQ=255) ggATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgcgc < 1:1985048/71‑1 (MQ=255) gatgatCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGca > 1:2693299/1‑71 (MQ=255) tgatCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAg > 1:579648/1‑46 (MQ=255) tgatCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGcacc < 1:2918792/71‑1 (MQ=255) atCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGtttt < 1:474083/57‑1 (MQ=255) ctctTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACAt > 1:759975/1‑71 (MQ=255) | GTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACAT > Exported/939509‑939646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |