| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 939,579 | T→A | 7.7% | E398D (GAA→GAT) | sfcA ← | malate dehydrogenase, NAD‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 939,579 | 0 | T | A | 7.7% | 304.9 / 13.6 | 104 | E398D (GAA→GAT) | sfcA | malate dehydrogenase, NAD‑requiring |
| Reads supporting (aligned to +/- strand): ref base T (52/44); new base A (5/3); total (57/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
GTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATC > Exported/939509‑939647 | gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct > 1:3402552/1‑71 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:3371332/71‑1 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct > 1:85636/1‑71 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct > 1:3125106/1‑71 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:3270018/71‑1 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:1806356/71‑1 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct > 1:1752243/1‑71 (MQ=255)gTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:1658478/71‑1 (MQ=255) gACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTc > 1:1936274/1‑71 (MQ=255) gACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTc > 1:3145206/1‑71 (MQ=255) aCGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTc < 1:2894225/70‑1 (MQ=255) cGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCg > 1:2270259/1‑71 (MQ=255) cGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCg < 1:3053179/71‑1 (MQ=255) gTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt < 1:436905/71‑1 (MQ=255) gTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt < 1:1424890/71‑1 (MQ=255) tCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTcc < 1:3044415/68‑1 (MQ=255) tCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTa < 1:1907867/71‑1 (MQ=255) gggTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGt > 1:3501467/1‑68 (MQ=255) tAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGc > 1:1569689/1‑71 (MQ=255) aGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGcc > 1:3199670/1‑71 (MQ=255) aGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGcc > 1:3195252/1‑71 (MQ=255) gACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGcc < 1:2406643/70‑1 (MQ=255) aCAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCg > 1:2587784/1‑71 (MQ=255) aCAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCg < 1:2310588/71‑1 (MQ=255) aCAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCg > 1:558845/1‑71 (MQ=255) cAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGc > 1:719520/1‑67 (MQ=255) aGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATctct < 1:2024210/54‑1 (MQ=255) aGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt < 1:1030223/71‑1 (MQ=255) aGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt < 1:1239196/71‑1 (MQ=255) aGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt > 1:3645963/1‑71 (MQ=255) cGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCt < 1:3410231/71‑1 (MQ=255) ggCATCTCGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt > 1:1668960/1‑56 (MQ=25) ggCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTg < 1:891171/71‑1 (MQ=255) ggCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt > 1:107888/1‑56 (MQ=37) ggCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt > 1:3226233/1‑56 (MQ=37) ggCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGAAGAGATCATCCGt > 1:510680/1‑56 (MQ=37) cATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTc > 1:2123070/1‑71 (MQ=255) cATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTc > 1:2198769/1‑71 (MQ=255) tCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGcc < 1:1737588/60‑1 (MQ=255) tCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGcc < 1:102467/60‑1 (MQ=255) tCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCt > 1:319284/1‑71 (MQ=255) aCGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTa < 1:532188/51‑1 (MQ=255) aCGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga < 1:438895/71‑1 (MQ=255) aCGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga < 1:2389310/71‑1 (MQ=255) aCGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga < 1:2058904/71‑1 (MQ=255) aCGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga < 1:1928104/71‑1 (MQ=255) aCGAACGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTcc > 1:2138104/1‑68 (MQ=255) gATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCg < 1:2060658/47‑1 (MQ=255) tCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACg < 1:42438/71‑1 (MQ=255) cGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGc > 1:3523060/1‑71 (MQ=255) aCGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTaaa > 1:731882/1‑45 (MQ=255) aCGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCa < 1:2680453/70‑1 (MQ=255) cgcgGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAAt > 1:3393025/1‑71 (MQ=255) cgcgGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAAt > 1:2793243/1‑71 (MQ=255) cgcgGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAAt > 1:1879218/1‑71 (MQ=255) gcgGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCaa > 1:2808786/1‑69 (MQ=255) aCAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGaa > 1:2933318/1‑71 (MQ=255) aCAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGaa > 1:2660339/1‑71 (MQ=255) aCAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGaa > 1:1718944/1‑71 (MQ=255) aGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAata > 1:1932948/1‑71 (MQ=255) gtgtTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTg < 1:2118045/53‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCt < 1:3424025/44‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTg < 1:2823097/45‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTg < 1:2730102/45‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTg < 1:26887/45‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCt < 1:443004/70‑1 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTg > 1:2610544/1‑71 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTg > 1:1887167/1‑71 (MQ=255) tttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTg > 1:995438/1‑71 (MQ=255) ttATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTgag < 1:425991/51‑1 (MQ=255) tATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGt > 1:3246355/1‑71 (MQ=255) tATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGt > 1:620140/1‑71 (MQ=255) tATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGt > 1:84488/1‑71 (MQ=255) tGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGttt < 1:2503321/71‑1 (MQ=255) tGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGttt < 1:1582098/71‑1 (MQ=255) tGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGtt < 1:3545968/70‑1 (MQ=255) tGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGtt < 1:757259/70‑1 (MQ=255) cATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt > 1:3418191/1‑36 (MQ=255) cATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt > 1:1899240/1‑36 (MQ=255) cATCTCACGGATGATCTCTTCCGTAAACAGCCCGGt > 1:460512/1‑36 (MQ=255) cATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga > 1:1076166/1‑45 (MQ=255) cATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTga > 1:2406597/1‑45 (MQ=255) attctcACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTa < 1:2511526/69‑1 (MQ=255) aTCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTa < 1:2438811/70‑1 (MQ=255) tctcACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGaa < 1:1115718/57‑1 (MQ=255) cACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTg > 1:1920471/1‑71 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACa < 1:1870295/67‑1 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACAt < 1:3646009/68‑1 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgc > 1:2554372/1‑71 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgc > 1:2747206/1‑71 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgc > 1:1549046/1‑71 (MQ=255) aCGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgc > 1:2794910/1‑71 (MQ=255) cGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTgcg > 1:2566834/1‑71 (MQ=255) gatgatCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGca > 1:2693299/1‑71 (MQ=255) tgatCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGa > 1:2920373/1‑47 (MQ=255) atCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGtttt < 1:474083/57‑1 (MQ=255) ctctTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACAt > 1:759975/1‑71 (MQ=255) tctTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCt > 1:3377683/1‑48 (MQ=255) tctTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCt > 1:974832/1‑48 (MQ=255) tctTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATc > 1:2919695/1‑71 (MQ=255) | GTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATC > Exported/939509‑939647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |