| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 965,646 | C→T | 55.8% | G83D (GGC→GAC) | ydeM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 965,646 | 0 | C | T | 55.8% | 37.5 / 136.7 | 104 | G83D (GGC→GAC) | ydeM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (22/24); new base T (30/28); total (52/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCACCGCC > Exported/965581‑965716 | aGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCa < 1:2294799/71‑1 (MQ=255) ggCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCAt < 1:918566/71‑1 (MQ=255) gCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCa > 1:1379773/1‑69 (MQ=255) cacaCCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAg < 1:2230486/71‑1 (MQ=255) cacaCCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg > 1:1160082/1‑71 (MQ=255) cacaCCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg > 1:3233450/1‑71 (MQ=255) cacaCCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAg > 1:595288/1‑71 (MQ=255) cacCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCg > 1:1412253/1‑71 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgt > 1:836589/1‑71 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgt > 1:2126899/1‑71 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgt > 1:861831/1‑71 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgt > 1:1837632/1‑71 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGc > 1:282200/1‑64 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGc > 1:3041615/1‑64 (MQ=255) acCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCgt > 1:1471944/1‑71 (MQ=255) cattcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttg < 1:2324910/71‑1 (MQ=255) cattcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttg > 1:3141445/1‑71 (MQ=255) ttattCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGa > 1:889569/1‑69 (MQ=255) ttattCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGa < 1:322535/69‑1 (MQ=255) ttattCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATa < 1:856732/71‑1 (MQ=255) attCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATa > 1:1644636/1‑69 (MQ=255) ttCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGt < 1:718395/70‑1 (MQ=255) ttCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGt < 1:768092/70‑1 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgt < 1:3664399/55‑1 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgt < 1:1233288/55‑1 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATaa > 1:3231215/1‑71 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATaa > 1:3152228/1‑71 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATaa > 1:3405538/1‑71 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATaa > 1:427576/1‑71 (MQ=255) aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCa > 1:3140700/1‑47 (MQ=255) aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCa > 1:2048720/1‑47 (MQ=255) aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCa > 1:1425692/1‑47 (MQ=255) aaaTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACt < 1:2656712/71‑1 (MQ=255) aTGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACttt < 1:2974492/71‑1 (MQ=255) tGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTa < 1:2928653/71‑1 (MQ=255) tGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTa < 1:1576012/71‑1 (MQ=255) ccATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTAc < 1:770662/70‑1 (MQ=255) ccATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTAc < 1:3418062/70‑1 (MQ=255) ttCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGAt < 1:889246/52‑1 (MQ=255) ttCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGa > 1:2827984/1‑70 (MQ=255) cGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATa > 1:2155006/1‑51 (MQ=255) cGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAg > 1:3205939/1‑52 (MQ=255) cGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaa > 1:3151048/1‑70 (MQ=255) ttGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAAt > 1:847009/1‑71 (MQ=255) gTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATc < 1:1377980/70‑1 (MQ=255) tAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaaaa < 1:3616342/67‑1 (MQ=255) aTGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAgg > 1:1058291/1‑71 (MQ=255) tGCATTAAAAATACGTTTTTGGTCTGCATAGCgttgtt < 1:3538283/38‑1 (MQ=255) tGCATTAAAAATACGTTTTTGGTCTGCATAGCgttgtt < 1:3418136/38‑1 (MQ=255) tGCATTAAAAATACGTTTTTGGTCTGCATAGCgttgtt < 1:146651/38‑1 (MQ=255) tGCATTAAAAATACGTTTTTGGTCTGCATAGCgttgtt < 1:3267685/38‑1 (MQ=255) gCATTAAAAATACGTTTTTGGTCTGCATAGCgttgtt < 1:1569631/37‑1 (MQ=255) cATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAg > 1:969982/1‑67 (MQ=255) cATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAg > 1:3591048/1‑67 (MQ=255) cATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATa > 1:63131/1‑40 (MQ=255) cATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATa > 1:2431729/1‑40 (MQ=255) cATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc > 1:1740555/1‑69 (MQ=255) aTTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAAt < 1:1504455/62‑1 (MQ=255) aTTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccag > 1:41577/1‑71 (MQ=255) aTTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTAc > 1:3315032/1‑53 (MQ=255) aTTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTAc > 1:2289498/1‑53 (MQ=255) aTTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTAc > 1:1775348/1‑53 (MQ=255) ttAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATcc < 1:1235029/63‑1 (MQ=255) ttAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATcc < 1:257423/63‑1 (MQ=255) ttAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATcc < 1:1932496/63‑1 (MQ=255) ttAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc < 1:1289711/71‑1 (MQ=255) ttAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgcca > 1:776777/1‑69 (MQ=255) ttAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAg < 1:1727315/65‑1 (MQ=255) tAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACttt > 1:2846876/1‑49 (MQ=255) tAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:1808436/1‑70 (MQ=255) aaaaaTATGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:54094/1‑69 (MQ=255) aaaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAAt > 1:1675164/1‑59 (MQ=255) aaaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagcc < 1:2363473/70‑1 (MQ=255) aaaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAgg > 1:3568679/1‑64 (MQ=255) aaaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAgg > 1:2027806/1‑64 (MQ=255) aaaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagcc < 1:3627616/70‑1 (MQ=255) aaaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:551480/1‑69 (MQ=255) aaaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgccagc > 1:2904225/1‑69 (MQ=255) aaaaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGccagccag < 1:1625281/71‑1 (MQ=255) aaTACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCaga < 1:2294344/70‑1 (MQ=255) aTACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGt > 1:3013730/1‑71 (MQ=255) tACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTg < 1:3487346/71‑1 (MQ=255) cGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTggg < 1:3617115/71‑1 (MQ=255) cGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTggg < 1:1403773/71‑1 (MQ=255) cGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTggg < 1:1408330/71‑1 (MQ=255) gTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGt < 1:3378761/71‑1 (MQ=255) gTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGt < 1:2385286/71‑1 (MQ=255) gTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGt > 1:2169794/1‑71 (MQ=255) gTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACt < 1:709262/38‑1 (MQ=255) gTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACt < 1:698895/38‑1 (MQ=255) gTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACt < 1:3613678/38‑1 (MQ=255) gTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACt < 1:2266307/38‑1 (MQ=255) gTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACt < 1:1287735/38‑1 (MQ=255) gTTTTTGGCCTGCATAGCGTTCTTGATAGTGAATAACt < 1:2466087/38‑1 (MQ=255) tttttGGCGTGCATAGCGTTGTTGATAGTGATTAACt < 1:2574610/37‑1 (MQ=38) tttttGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaa < 1:335160/47‑1 (MQ=255) tttGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTc > 1:3400001/1‑70 (MQ=255) ttGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCa < 1:3548715/70‑1 (MQ=255) ggCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCAcc < 1:1760396/70‑1 (MQ=255) ccTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCACCGcc < 1:2536177/71‑1 (MQ=255) | AGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTTCACCGCC > Exported/965581‑965716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |