| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,014,719 | T→C | 5.5% | Q450Q (CAA→CAG) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,014,719 | 0 | T | C | 5.5% | 272.9 / 4.7 | 91 | Q450Q (CAA→CAG) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (51/35); new base C (3/2); total (54/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCG > Exported/1014651‑1014786 | gCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTg < 1:1115746/71‑1 (MQ=255) aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:256349/1‑71 (MQ=255) aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:688963/1‑71 (MQ=255) aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCACCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:1528744/1‑70 (MQ=255) aTCCGTCACCTGCTGGCTGCGGTTGCCTTTAGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:1630231/1‑71 (MQ=255) tCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATc > 1:3298645/1‑71 (MQ=255) tCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATc > 1:2316786/1‑71 (MQ=255) cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCg < 1:3010248/70‑1 (MQ=255) cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGa < 1:96148/71‑1 (MQ=255) tCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGtt < 1:1729560/63‑1 (MQ=255) cACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACa > 1:1543197/1‑70 (MQ=255) aCCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACAt > 1:3553830/1‑70 (MQ=255) aCCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCAcg > 1:1696151/1‑68 (MQ=255) tgctgGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGa > 1:3612077/1‑64 (MQ=255) tgctgGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg > 1:1654041/1‑70 (MQ=255) tgctgGCTGCGGTTGCCTTTCGCCAGCATGATCAAACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg > 1:2290286/1‑70 (MQ=255) ctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGagt > 1:1372482/1‑69 (MQ=255) ctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGagt > 1:3391991/1‑69 (MQ=255) gctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa < 1:3372384/55‑1 (MQ=255) gctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa < 1:3376674/55‑1 (MQ=255) gctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatcc < 1:1438703/70‑1 (MQ=255) cctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCTCCCTGCGCTTGCAGTTGATCGACATAAGAatcca < 1:2763591/70‑1 (MQ=255) ctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa < 1:1423252/54‑1 (MQ=255) ctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:354527/67‑1 (MQ=255) ctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:2274862/67‑1 (MQ=255) tgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATc > 1:516534/1‑55 (MQ=255) tgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATc > 1:2548659/1‑55 (MQ=255) tgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatc > 1:2617295/1‑71 (MQ=255) gTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtc < 1:2894135/71‑1 (MQ=255) gTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtc < 1:3149736/71‑1 (MQ=255) ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGAc < 1:666455/53‑1 (MQ=255) accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtc < 1:897784/59‑1 (MQ=255) accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtc < 1:3541972/59‑1 (MQ=255) ccTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGtt < 1:1483595/43‑1 (MQ=255) ccTTTCGCCAGCATGATCATACTTCCGCCCTGCCCTTGCAGTTGATCGACATAAGAATCCATCCGTCcgg < 1:3420205/70‑1 (MQ=255) cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGaa < 1:2405443/56‑1 (MQ=255) cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGaa < 1:262842/56‑1 (MQ=255) cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcggc < 1:1962246/70‑1 (MQ=255) ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATaa > 1:3639728/1‑51 (MQ=255) ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATaa > 1:1834072/1‑51 (MQ=255) ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt > 1:1426070/1‑71 (MQ=255) ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt > 1:3312496/1‑71 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:2633440/1‑41 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:3512581/1‑41 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:3466974/1‑41 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:1579359/1‑41 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:2329721/1‑41 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc > 1:1988346/1‑71 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc > 1:2096966/1‑71 (MQ=255) tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc > 1:2218883/1‑71 (MQ=255) gCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt > 1:1852286/1‑71 (MQ=255) ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGtt < 1:1223378/71‑1 (MQ=255) cAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg > 1:3237529/1‑71 (MQ=255) cAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg < 1:1549984/71‑1 (MQ=255) cAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg < 1:1977890/71‑1 (MQ=255) cAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg < 1:849755/71‑1 (MQ=255) cAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCAATCCGTCCGGCGGTCGTTg > 1:3627355/1‑71 (MQ=255) gCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt > 1:1118202/1‑67 (MQ=255) cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc < 1:765202/71‑1 (MQ=255) aTGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc < 1:2368238/71‑1 (MQ=255) gATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc > 1:2595611/1‑69 (MQ=255) gATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc > 1:1274646/1‑69 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:2606145/62‑1 (MQ=255) aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAag < 1:2876656/71‑1 (MQ=255) aTACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa < 1:664880/67‑1 (MQ=255) tACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGaa < 1:1289561/71‑1 (MQ=255) cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcgg > 1:1447578/1‑49 (MQ=255) cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcgg > 1:1524483/1‑49 (MQ=255) cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcgg > 1:1099523/1‑49 (MQ=255) cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc > 1:763165/1‑61 (MQ=255) cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc > 1:2426018/1‑61 (MQ=255) cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc > 1:2532796/1‑61 (MQ=255) cTTCCGCCCTGCGCTTGCAGTTGACCGACATAAGAATCCAtccgtccg > 1:1929395/1‑48 (MQ=255) ttCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:3365301/1‑71 (MQ=255) ttCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:2218762/1‑71 (MQ=255) ccccccTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:1393359/4‑69 (MQ=255) ccGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg > 1:1079546/1‑55 (MQ=255) ccGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:2116742/1‑69 (MQ=255) cGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg < 1:110734/54‑1 (MQ=255) cccTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAgg > 1:3631389/1‑70 (MQ=255) ccTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCa > 1:959778/1‑71 (MQ=255) cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCAt < 1:2673727/71‑1 (MQ=255) tGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCAt > 1:1849560/1‑70 (MQ=255) tGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCAt > 1:2384967/1‑70 (MQ=255) cTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCt < 1:1257919/71‑1 (MQ=255) cTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCt < 1:3012474/71‑1 (MQ=255) ttGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGa < 1:2219880/60‑1 (MQ=255) tGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc < 1:2071370/46‑1 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGaa > 1:680422/1‑53 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGaa > 1:1068468/1‑53 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTc < 1:3379689/70‑1 (MQ=255) gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTc < 1:1647673/70‑1 (MQ=255) cAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCg > 1:2766370/1‑71 (MQ=255) ttGATCGTCATAAGAATCCATCCGTCCGGCGGTCGTTggg < 1:3111988/40‑1 (MQ=255) | GCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCG > Exported/1014651‑1014786 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |