| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,014,818 | A→G | 7.4% | H417H (CAT→CAC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,014,818 | 0 | A | G | 7.4% | 194.0 / 5.3 | 67 | H417H (CAT→CAC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (26/36); new base G (3/2); total (29/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATA > Exported/1014761‑1014887 | aaGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACGCGCGTAGTAGATCGGGTGATCTTTGATGt < 1:2619708/71‑1 (MQ=255)aaGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATg > 1:101962/1‑70 (MQ=255)aaGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATg > 1:3567164/1‑70 (MQ=255)aaGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGt < 1:1732437/71‑1 (MQ=255)aaGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGt < 1:113606/71‑1 (MQ=255) agagAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGt < 1:3653243/70‑1 (MQ=255) gaACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTg < 1:611043/71‑1 (MQ=255) gaACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTg > 1:1948386/1‑71 (MQ=255) aaCCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGc > 1:2491710/1‑71 (MQ=255) ccGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGt < 1:736646/64‑1 (MQ=255) gAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa > 1:584844/1‑62 (MQ=255) gAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCgg > 1:1275875/1‑68 (MQ=255) gAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCgg > 1:2820544/1‑68 (MQ=255) ggCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGcc > 1:1218710/1‑71 (MQ=255) gCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCttt < 1:1371049/53‑1 (MQ=255) gCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCttt < 1:1933503/53‑1 (MQ=255) gCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGccc > 1:323446/1‑71 (MQ=255) aTAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCtt > 1:1414733/1‑71 (MQ=255) tAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATg < 1:812547/54‑1 (MQ=255) aaCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCt > 1:551355/1‑68 (MQ=255) aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGgatgat > 1:329019/1‑44 (MQ=255) aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTa > 1:993509/1‑54 (MQ=255) aCCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCtt > 1:1103119/1‑68 (MQ=255) ttCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCtt < 1:2461649/44‑1 (MQ=255) ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGtt < 1:2773770/70‑1 (MQ=255) ccGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGtt < 1:2375409/70‑1 (MQ=255) gCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGc < 1:1796592/53‑1 (MQ=255) gCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGc < 1:776656/53‑1 (MQ=255) gCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTat > 1:2638307/1‑69 (MQ=255) cGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTg < 1:1724404/40‑1 (MQ=255) cGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTg < 1:2583768/40‑1 (MQ=255) cGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTg < 1:541624/40‑1 (MQ=255) cGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTg < 1:1316758/40‑1 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGa > 1:367144/1‑37 (MQ=255) ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:2329281/1‑71 (MQ=255) tGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg < 1:3097906/70‑1 (MQ=255) tGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg < 1:2192979/70‑1 (MQ=255) gCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctc < 1:1342874/71‑1 (MQ=255) gCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctc < 1:1733072/71‑1 (MQ=255) cGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt < 1:2672719/71‑1 (MQ=255) cGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTTCAGCAGCCCTTCACCGTTATCCATCCGCTCtt > 1:2900480/1‑71 (MQ=255) ggACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt > 1:1026715/1‑71 (MQ=255) ggACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt > 1:1813195/1‑71 (MQ=255) ggACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt > 1:2808968/1‑71 (MQ=255) ggACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt > 1:139355/1‑71 (MQ=255) ggACCCGCGTAGTAAATAGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCttt > 1:1368526/1‑71 (MQ=255) gACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCa > 1:2155113/1‑42 (MQ=255) cccGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCa > 1:1869584/1‑70 (MQ=255) ccGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGt < 1:2727957/71‑1 (MQ=255) ccGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGt < 1:130702/71‑1 (MQ=255) cgcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGtt < 1:1907587/71‑1 (MQ=255) gcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt > 1:2412296/1‑71 (MQ=255) gcgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:2126898/71‑1 (MQ=255) cgTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTg < 1:3120526/71‑1 (MQ=255) gtagtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc < 1:2240471/64‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTGACCGtt < 1:475581/45‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGtt < 1:1022622/45‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGtt < 1:2298116/45‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:3463488/71‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:1039109/71‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:1711950/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:2491895/71‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:2272110/71‑1 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCg > 1:1540795/1‑40 (MQ=255) aTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGc < 1:3033100/71‑1 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGc > 1:613750/1‑70 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCa < 1:3498900/71‑1 (MQ=255) cGGGTGATCTTTGATGTACTGCGGAAGTTCTTTAcc > 1:2327366/1‑36 (MQ=9) gatgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata < 1:1651201/71‑1 (MQ=255) | AAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATA > Exported/1014761‑1014887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |