| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 136,259 | A→C | 7.9% | I27M (ATT→ATG) | yadK ← | predicted fimbrial‑like adhesin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 136,259 | 0 | A | C | 7.9% | 190.8 / 12.3 | 76 | I27M (ATT→ATG) | yadK | predicted fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base A (38/32); new base C (4/2); total (42/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
GTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAG > Exported/136193‑136329 | gTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGTAACAAGGTCAATATCCTGTCCAGCAATgg < 1:2031585/71‑1 (MQ=255)gTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATg < 1:324417/70‑1 (MQ=255) ttACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGc < 1:2589782/71‑1 (MQ=255) tCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATgg < 1:813199/62‑1 (MQ=255) tCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATgg < 1:3234884/62‑1 (MQ=255) gCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGa > 1:1664984/1‑71 (MQ=255) ttttGCAGGTGCTGTTTTTTACATTGGCAATAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCg < 1:2293110/68‑1 (MQ=255) ttttGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAt > 1:3072456/1‑70 (MQ=255) ttttGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATg > 1:1878671/1‑71 (MQ=255) ttttGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATg > 1:3146144/1‑71 (MQ=255) ttGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGc > 1:1298106/1‑70 (MQ=255) gCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCaa > 1:2832105/1‑70 (MQ=255) cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCaaaa < 1:1974341/71‑1 (MQ=255) aGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGa < 1:816976/56‑1 (MQ=255) gCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCaa > 1:2239198/1‑44 (MQ=255) cTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAATCAGTaa < 1:474191/71‑1 (MQ=255) cTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTaa > 1:2257454/1‑71 (MQ=255) cTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTaa < 1:1929242/71‑1 (MQ=255) cTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTa < 1:3296452/70‑1 (MQ=255) gTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTg < 1:3165427/47‑1 (MQ=255) gTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTg < 1:1872986/47‑1 (MQ=255) gTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTg < 1:3060915/47‑1 (MQ=255) ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAt > 1:2844503/1‑56 (MQ=255) ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGAt > 1:2118535/1‑56 (MQ=255) ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATg > 1:1980010/1‑57 (MQ=255) ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATg < 1:3647946/70‑1 (MQ=255) ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGa > 1:2227807/1‑71 (MQ=255) tttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGa > 1:825858/1‑70 (MQ=255) ttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAg > 1:236347/1‑47 (MQ=255) ttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCaaa < 1:1809708/57‑1 (MQ=255) ttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACa > 1:2537597/1‑60 (MQ=255) ttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacag > 1:563596/1‑70 (MQ=255) ttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacag > 1:1426440/1‑70 (MQ=255) ttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacag > 1:14904/1‑70 (MQ=255) ttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacag > 1:1337246/1‑70 (MQ=255) aCATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTa < 1:3678544/61‑1 (MQ=255) cATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTg > 1:140765/1‑39 (MQ=255) cATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaa > 1:1393415/1‑70 (MQ=255) tGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTaa < 1:1224425/58‑1 (MQ=255) gCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAat > 1:251632/1‑71 (MQ=255) gCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAat > 1:1760066/1‑71 (MQ=255) aacaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACCGAatta < 1:474922/71‑1 (MQ=255) aacaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAatta > 1:3522967/1‑71 (MQ=255) acaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattat > 1:172814/1‑71 (MQ=255) caaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt < 1:390851/71‑1 (MQ=255) aGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatta > 1:2902860/1‑69 (MQ=255) ggTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaaca < 1:2448308/60‑1 (MQ=255) ggTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaaca < 1:2909496/60‑1 (MQ=255) tCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacaga > 1:3414703/1‑60 (MQ=255) tCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCtt > 1:1595349/1‑71 (MQ=255) cAATATCCTGTCCAGCAATGGCTGGACAGGCGATg > 1:644971/1‑35 (MQ=255) cAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacag < 1:955396/58‑1 (MQ=255) cAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacag < 1:1754258/58‑1 (MQ=255) cAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCtt > 1:2992987/1‑70 (MQ=255) cAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCtt > 1:1881557/1‑70 (MQ=255) aaTATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacagaa > 1:2301180/1‑59 (MQ=255) aaTATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtc < 1:1364588/70‑1 (MQ=255) aaTATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtc < 1:2213819/70‑1 (MQ=255) atatCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcat > 1:3084119/1‑71 (MQ=255) atatCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcat < 1:346318/71‑1 (MQ=255) tGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacag < 1:257604/50‑1 (MQ=255) gTCCAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcatc > 1:1912543/1‑65 (MQ=255) gTCCAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtcat > 1:586292/1‑64 (MQ=255) gTCCAGCAATGGCTGGCCAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCtt > 1:2524142/1‑70 (MQ=255) gTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTa > 1:946270/1‑71 (MQ=255) ccAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTctct < 1:1076981/58‑1 (MQ=39) ccAGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTctct < 1:3724718/58‑1 (MQ=39) cAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGt > 1:2047619/1‑71 (MQ=255) aGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtc > 1:1224322/1‑58 (MQ=39) aGCCATTGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtc > 1:1120112/1‑58 (MQ=39) aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGtt > 1:3478640/1‑71 (MQ=255) aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGt > 1:1939872/1‑70 (MQ=255) aGCAATGGCGGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTtca < 1:2496193/59‑1 (MQ=255) cAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGtt > 1:3532899/1‑69 (MQ=255) cAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGa < 1:1624073/71‑1 (MQ=255) cAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGa < 1:1462950/71‑1 (MQ=255) cAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGa < 1:3051277/71‑1 (MQ=255) aaTGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGa < 1:3242786/70‑1 (MQ=255) aaTGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAg > 1:2261140/1‑71 (MQ=255) aaTGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAg < 1:631974/71‑1 (MQ=255) | GTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAG > Exported/136193‑136329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |