| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,078 | G→C | 5.6% | Q331E (CAG→GAG) ‡ | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,078 | 0 | G | C | 5.6% | 224.8 / 3.4 | 71 | Q331E (CAG→GAG) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (50/17); new base C (2/2); total (52/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGC > Exported/1015010‑1015147 | cGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGAc > 1:2917931/1‑71 (MQ=255) cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:3614383/71‑1 (MQ=255) aGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCtt > 1:3142280/1‑71 (MQ=255) aGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCtt > 1:1833381/1‑71 (MQ=255) gCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTg < 1:2010917/58‑1 (MQ=255) tCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:66194/1‑71 (MQ=255) tCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt > 1:1476044/1‑71 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCGCTGACGGTTGATCTTCGCttt < 1:1265051/70‑1 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTg < 1:1140110/59‑1 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTg < 1:284169/59‑1 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:1505645/70‑1 (MQ=255) ccGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:180891/70‑1 (MQ=255) atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGatat < 1:3536867/70‑1 (MQ=255) atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATAtt < 1:951932/71‑1 (MQ=255) atTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:730401/1‑49 (MQ=255) atTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:2805781/1‑49 (MQ=255) tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt > 1:2682209/1‑71 (MQ=255) ttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGt > 1:1602294/1‑46 (MQ=255) tGCCTGGATTATGTTCCAGTTTTTCGATCAAGATCCCCTGaag > 1:1031139/1‑41 (MQ=38) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGt > 1:202822/1‑41 (MQ=255) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGt > 1:447447/1‑41 (MQ=255) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGt > 1:3570709/1‑41 (MQ=255) tGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:2317259/1‑68 (MQ=255) gATTATGTTCCAGTTTTTGGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:1421823/1‑71 (MQ=255) gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:2602334/71‑1 (MQ=255) aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:3226318/1‑70 (MQ=255) aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:463143/1‑70 (MQ=255) aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:2092071/70‑1 (MQ=255) ttATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGAt > 1:1871937/1‑41 (MQ=255) tgtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag > 1:519123/3‑71 (MQ=255) tATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:1293695/1‑51 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:3240928/1‑69 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:788041/1‑69 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag > 1:2833994/1‑69 (MQ=255) tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:3470646/71‑1 (MQ=255) tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2387993/1‑60 (MQ=255) tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:2123218/1‑58 (MQ=255) cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga > 1:1528039/1‑66 (MQ=255) cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga > 1:2236462/1‑66 (MQ=255) cAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCttt < 1:1390731/42‑1 (MQ=19) aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATAtt < 1:1435428/47‑1 (MQ=255) aGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAatg > 1:1878441/1‑44 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:1161778/39‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCttt < 1:2638609/39‑1 (MQ=255) tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa < 1:2873524/71‑1 (MQ=255) ttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc < 1:3178995/65‑1 (MQ=255) tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:485722/1‑71 (MQ=255) tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAt < 1:1496480/70‑1 (MQ=255) tCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:2360816/1‑61 (MQ=255) tCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATacc > 1:3622661/1‑71 (MQ=255) cGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACg < 1:2870580/62‑1 (MQ=255) cGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATacc > 1:2623169/1‑70 (MQ=255) gATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATac > 1:2664655/1‑68 (MQ=255) aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGcc > 1:2033016/1‑62 (MQ=255) aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:3142814/1‑71 (MQ=255) aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:1527639/1‑71 (MQ=255) aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:858511/1‑71 (MQ=255) aTCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgac > 1:790025/1‑71 (MQ=255) tCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgacc > 1:916533/1‑71 (MQ=255) ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:3629084/1‑37 (MQ=255) ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa > 1:3496109/1‑37 (MQ=255) ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:1643423/1‑71 (MQ=255) aGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg > 1:304036/1‑71 (MQ=255) aGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCggg > 1:1920539/1‑71 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACgg > 1:519633/1‑35 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:1458843/1‑39 (MQ=255) tCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATa > 1:2240153/1‑59 (MQ=255) ccccTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2147126/1‑40 (MQ=255) ccccTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:251179/1‑40 (MQ=255) tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccga > 1:2903237/1‑58 (MQ=255) tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccga > 1:1248502/1‑58 (MQ=255) tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc > 1:943717/1‑71 (MQ=255) | CGCTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGC > Exported/1015010‑1015147 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |