Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,063,788 T→A 8.2% intergenic (+138/+24) sodB → / ← ydhP superoxide dismutase, Fe/predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,063,7880TA 8.2% 225.2 / 10.3 85intergenic (+138/+24)sodB/ydhPsuperoxide dismutase, Fe/predicted transporter
Reads supporting (aligned to +/- strand):  ref base T (25/53);  new base A (2/5);  total (27/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

GGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGA  >  Exported/1063719‑1063856
                                                                     |                                                                    
ggTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGt                                                                      <  1:3336933/70‑1 (MQ=255)
ggTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGt                                                                      <  1:929266/70‑1 (MQ=255)
 gTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTa                                                                    <  1:2675476/71‑1 (MQ=255)
    ggATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATcc                                                                 >  1:3079946/1‑71 (MQ=255)
    ggATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATcc                                                                 >  1:2498761/1‑71 (MQ=255)
     gATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCt                                                                <  1:1030144/71‑1 (MQ=255)
      aTAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCtt                                                               >  1:656590/1‑71 (MQ=255)
      aTAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCtt                                                               >  1:894050/1‑71 (MQ=255)
       tAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTc                                                              >  1:383870/1‑71 (MQ=255)
        aaGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCt                                                             >  1:741099/1‑71 (MQ=255)
         aGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCt                                                             <  1:2193958/70‑1 (MQ=255)
           gCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCg                                                          <  1:2851598/71‑1 (MQ=255)
           gCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCg                                                          <  1:1405999/71‑1 (MQ=255)
           gCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCg                                                          <  1:1893838/71‑1 (MQ=255)
            cACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGt                                                         >  1:2781298/1‑71 (MQ=255)
            cACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGt                                                         >  1:2526112/1‑71 (MQ=255)
            cACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGt                                                         >  1:2427126/1‑71 (MQ=255)
            cACTCGCCGCATCCGGCAAGATAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGt                                                         >  1:549191/1‑70 (MQ=255)
             aCTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGtt                                                        >  1:1114175/1‑71 (MQ=255)
             aCTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGtt                                                        >  1:524546/1‑71 (MQ=255)
              cTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTTTAACGCAGAAGGATa                                                                    <  1:568150/58‑1 (MQ=37)
              cTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGttt                                                       <  1:2137351/71‑1 (MQ=255)
              cTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATa                                                                    <  1:2923103/58‑1 (MQ=255)
              cTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATa                                                                    <  1:2914341/58‑1 (MQ=255)
              cTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATa                                                                    <  1:1746400/58‑1 (MQ=255)
               tCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCt                                                             >  1:3114461/1‑64 (MQ=255)
               tCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCt                                                             >  1:682586/1‑64 (MQ=255)
                cgccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATaac                                                                  >  1:1537609/1‑56 (MQ=255)
                 gccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGGGttttt                                                     <  1:2148/70‑1 (MQ=255)
                 gccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGttttt                                                     <  1:1277436/70‑1 (MQ=255)
                 gccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTg                                                    <  1:2611170/71‑1 (MQ=255)
                 gccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTg                                                    <  1:2527367/71‑1 (MQ=255)
                 gccgcATCCGGCAAGATAAATCGCACGTTGTCAACAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTg                                                    <  1:2890819/71‑1 (MQ=255)
                  cggcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTAt                                                                   <  1:1145610/53‑1 (MQ=255)
                  ccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTAt                                                                   <  1:56468/55‑1 (MQ=255)
                  ccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTAt                                                                   <  1:736436/55‑1 (MQ=255)
                  ccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                   <  1:3067740/71‑1 (MQ=255)
                  ccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                   >  1:1726379/1‑71 (MQ=255)
                  ccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                   <  1:2464267/71‑1 (MQ=255)
                  ccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                   <  1:847842/71‑1 (MQ=255)
                  ccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                   <  1:3358290/71‑1 (MQ=255)
                    gcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCt                                                             >  1:395814/1‑59 (MQ=255)
                     cATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTa                                                <  1:914608/71‑1 (MQ=255)
                       tCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAAt                                              <  1:1859834/71‑1 (MQ=255)
                            cAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCt                                         <  1:229316/71‑1 (MQ=255)
                              aGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGt                                       <  1:2759130/71‑1 (MQ=255)
                              aGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGt                                       <  1:1041198/71‑1 (MQ=255)
                              aGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCt                                                             <  1:1701481/49‑1 (MQ=37)
                               gATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGtt                                      <  1:136530/71‑1 (MQ=255)
                                 tAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCg                                                          >  1:2088153/1‑49 (MQ=255)
                                 tAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCg                                                          >  1:2945692/1‑49 (MQ=255)
                                  aaaTCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAg                                    >  1:1183466/1‑70 (MQ=255)
                                   aaTCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCa                                  <  1:1869592/71‑1 (MQ=255)
                                     tCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAAc                                <  1:3376261/71‑1 (MQ=255)
                                     tCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAAc                                <  1:697378/71‑1 (MQ=255)
                                      cGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACg                               >  1:970196/1‑71 (MQ=255)
                                       gCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACg                               >  1:1791545/1‑70 (MQ=255)
                                             tGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACt                         >  1:1913768/1‑70 (MQ=255)
                                             tGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                        >  1:443698/1‑71 (MQ=255)
                                               tCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGtt                      <  1:629733/71‑1 (MQ=255)
                                               tCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGt                       <  1:276166/70‑1 (MQ=255)
                                                 aGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGc                                          >  1:2140362/1‑49 (MQ=255)
                                                 aGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGttt                     >  1:2330616/1‑70 (MQ=255)
                                                 aGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTc                    <  1:2851031/71‑1 (MQ=255)
                                                 aGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTc                    <  1:3660045/71‑1 (MQ=255)
                                                    aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAgg                 <  1:907966/71‑1 (MQ=255)
                                                    aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAgg                 <  1:3431279/71‑1 (MQ=255)
                                                     aCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgt                >  1:179288/1‑71 (MQ=255)
                                                         tAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTTTTAGCAACGCAAACTGTTTCAGgttg              <  1:349837/69‑1 (MQ=255)
                                                         tAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgttg              <  1:2251236/69‑1 (MQ=255)
                                                         tAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgttg              <  1:2228277/69‑1 (MQ=255)
                                                         tAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgttg              <  1:26781/69‑1 (MQ=255)
                                                           aCGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAAtt                                             <  1:1037399/36‑1 (MQ=255)
                                                           aCGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCTACGCAAACTGTTTCAGGTTGtttt          <  1:646414/71‑1 (MQ=255)
                                                           aCGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGtttt          <  1:78125/71‑1 (MQ=255)
                                                           aCGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGtttt          <  1:1695840/71‑1 (MQ=255)
                                                           aCGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGtttt          <  1:1430392/71‑1 (MQ=255)
                                                           aCGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGttt           <  1:2046584/70‑1 (MQ=255)
                                                            cGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTc         <  1:3440562/71‑1 (MQ=255)
                                                            cGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTc         <  1:1784101/71‑1 (MQ=255)
                                                              cAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAg                  <  1:1474315/60‑1 (MQ=255)
                                                              cAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTg       <  1:5392/71‑1 (MQ=255)
                                                               aGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg      <  1:247326/71‑1 (MQ=255)
                                                               aGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg      <  1:2518685/71‑1 (MQ=255)
                                                                 aaGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCt        <  1:514305/67‑1 (MQ=255)
                                                                   ggTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGa  <  1:313764/71‑1 (MQ=255)
                                                                   ggTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGa  <  1:2798319/71‑1 (MQ=255)
                                                                     |                                                                    
GGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGA  >  Exported/1063719‑1063856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: