Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,187,540 G→A 34.9% G10R (GGG→AGG)  yeaI → predicted diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,187,5400GA34.9% 44.3 / 39.2 43G10R (GGG→AGG) yeaIpredicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (12/16);  new base A (6/9);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCT  >  Exported/1187471‑1187604
                                                                     |                                                                
aCCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATggg                                                                 <  1:2343599/71‑1 (MQ=255)
aCCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATggg                                                                 <  1:1911940/71‑1 (MQ=255)
aCCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATgg                                                                  <  1:2600082/70‑1 (MQ=255)
aCCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATgg                                                                  <  1:153846/70‑1 (MQ=255)
  cTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGt                                                               <  1:1917963/71‑1 (MQ=255)
          aaTTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCtt                                                        >  1:581265/1‑70 (MQ=255)
           aTTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCttt                                                       <  1:14028/70‑1 (MQ=255)
                   ccAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAttt                                               <  1:3433460/70‑1 (MQ=255)
                    cAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAtttt                                              <  1:2327924/70‑1 (MQ=255)
                    cAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAtttt                                              <  1:3545269/70‑1 (MQ=255)
                    cAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAtttt                                              <  1:3455125/70‑1 (MQ=255)
                     aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa                                                             <  1:1749685/54‑1 (MQ=255)
                     aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa                                                             <  1:2565536/54‑1 (MQ=255)
                     aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa                                                             <  1:3128597/54‑1 (MQ=255)
                     aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa                                                             <  1:1896080/54‑1 (MQ=255)
                     aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa                                                             <  1:1569540/54‑1 (MQ=255)
                     aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa                                                             <  1:1126344/54‑1 (MQ=255)
                     aGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGCTTa                                                             <  1:1387982/54‑1 (MQ=255)
                        aaaCAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTAt                                          <  1:898574/70‑1 (MQ=255)
                         aaCAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCg                                        >  1:2159134/1‑71 (MQ=255)
                         aaCAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCg                                        <  1:2747606/71‑1 (MQ=255)
                          aCAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCg                                        <  1:582071/70‑1 (MQ=255)
                          aCAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGAGGTTa                                                             <  1:1421674/49‑1 (MQ=255)
                            aaTCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTa                                     <  1:1854261/71‑1 (MQ=255)
                               cATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATAtt                                                <  1:1732225/57‑1 (MQ=255)
                                  tCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGaa                                <  1:934417/70‑1 (MQ=255)
                                       aaaaTGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGa                          >  1:1774024/1‑71 (MQ=255)
                                       aaaaTGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGa                          <  1:1186799/71‑1 (MQ=255)
                                        aaaTGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGa                          >  1:2910027/1‑70 (MQ=255)
                                        aaaTGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGAt                         >  1:1893100/1‑71 (MQ=255)
                                           tGATTCAGTCAACACGTATTTCCATGGGGTTATTCt                                                         >  1:1159983/1‑36 (MQ=255)
                                           tGATTCAGTCAACACGTATTTCCATGAGGTTATTCtt                                                        >  1:2501842/1‑37 (MQ=255)
                                           tGATTCAGTCAACACGTATTTCCATGAGGTTATTCtt                                                        >  1:2104378/1‑37 (MQ=255)
                                           tGATTCAGTCAACACGTATTTCCATGAGGTTATTCtt                                                        >  1:1554628/1‑37 (MQ=255)
                                           tGATTCAGTCAACACGTATTTCCATGAGGTTATTCtt                                                        >  1:1168397/1‑37 (MQ=255)
                                                 aGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCg                                        <  1:1846575/47‑1 (MQ=255)
                                                 aGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCaa                <  1:1529208/71‑1 (MQ=255)
                                                   tCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGtt                                      >  1:811984/1‑47 (MQ=255)
                                                   tCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGtt                                      >  1:881547/1‑47 (MQ=255)
                                                      acacGTATTTCCATGAGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACta           <  1:1513902/71‑1 (MQ=255)
                                                       cacGTATTTCCATGAGGTTATTCTTTAAATAtttttt                                            >  1:912957/1‑37 (MQ=255)
                                                        acGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATc                        >  1:93423/1‑56 (MQ=255)
                                                          gTATTTCCATGAGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACtatata       >  1:3296496/1‑71 (MQ=255)
                                                             tttCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACt            <  1:1249647/63‑1 (MQ=255)
                                                             tttCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATAtct    >  1:1307647/1‑71 (MQ=255)
                                                              ttCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATAtctc   >  1:3428896/1‑71 (MQ=255)
                                                               tCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATctct  >  1:1927544/1‑71 (MQ=255)
                                                                     |                                                                
ACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTCCATGGGGTTATTCTTTAAATATTTTTTATCGTTAACGAAAATTGATCCTGGTCAAAACTATATATCTCT  >  Exported/1187471‑1187604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: