Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,201,033 C→T 6.3% T62T (ACC→ACT)  sdaA → L‑serine deaminase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,201,0330CT 6.3% 308.3 / 6.6 95T62T (ACC→ACT) sdaAL‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base C (48/40);  new base T (3/3);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGT  >  Exported/1200965‑1201102
                                                                    |                                                                     
taTAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCg                                                                      <  1:3321826/69‑1 (MQ=255)
gATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCg                                                                      <  1:2702588/70‑1 (MQ=255)
 aTAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGat                                                                    <  1:390352/71‑1 (MQ=255)
 aTAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGat                                                                    <  1:1836072/71‑1 (MQ=255)
  tAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGat                                                                    >  1:409323/1‑70 (MQ=255)
   gacGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGatat                                                                  <  1:828902/69‑1 (MQ=255)
       ttACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGc                                                               >  1:2622972/1‑70 (MQ=255)
       ttACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGc                                                               >  1:2580963/1‑70 (MQ=255)
        tACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGatat                                                                  >  1:2453327/1‑66 (MQ=255)
        tACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGatat                                                                  >  1:1472703/1‑66 (MQ=255)
        tACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGatat                                                                  >  1:1944530/1‑66 (MQ=255)
             gcgTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGatat                                                                  >  1:2881490/1‑61 (MQ=255)
             gcgTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGatat                                                                  >  1:1831232/1‑61 (MQ=255)
             gcgTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCattat                                                         <  1:3120614/70‑1 (MQ=255)
              cgTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCattatt                                                        <  1:1383815/70‑1 (MQ=255)
              cgTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCAttatta                                                       >  1:825405/1‑71 (MQ=255)
              cgTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCAttatta                                                       >  1:426758/1‑71 (MQ=255)
              cgTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCAttatta                                                       >  1:3031303/1‑71 (MQ=255)
                ttGCCGTGGACGTTTATGGTTCACTGTCGCTGACGTGTAAAGGCCACCACACCGATATCGCCatt                                                           <  1:2531128/65‑1 (MQ=255)
                ttGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATg                                                     <  1:362896/71‑1 (MQ=255)
                  gCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCattat                                                         >  1:2060943/1‑65 (MQ=255)
                   ccGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATggg                                                   >  1:2957989/1‑70 (MQ=255)
                    cGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTc                                                 <  1:3279030/71‑1 (MQ=255)
                     gttgACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCt                                                >  1:3112389/4‑71 (MQ=255)
                     gttgACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCt                                                >  1:3328081/4‑71 (MQ=255)
                      tGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCattat                                                         <  1:1606208/61‑1 (MQ=255)
                      tGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACAGATATCGCCattat                                                         <  1:1572008/61‑1 (MQ=255)
                        gACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTg                                              <  1:1827697/70‑1 (MQ=255)
                        gACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGc                                             >  1:3353179/1‑71 (MQ=255)
                          cGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAg                                           >  1:3507445/1‑71 (MQ=255)
                            tttATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCttc                                              <  1:3356120/66‑2 (MQ=255)
                            tttATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGc                                             <  1:2731205/67‑1 (MQ=255)
                            tttATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGt                                         <  1:3514803/71‑1 (MQ=255)
                              tATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATgg                                                    >  1:1246290/1‑58 (MQ=255)
                              tATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATgg                                                    >  1:903857/1‑58 (MQ=255)
                                tGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAAc                                      >  1:3591813/1‑70 (MQ=255)
                                tGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAAc                                      >  1:579977/1‑70 (MQ=255)
                                 ggTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGcc                                                              <  1:3434033/45‑1 (MQ=255)
                                 ggTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGcc                                                              <  1:898676/45‑1 (MQ=255)
                                  gTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGTTAACGaa                                   <  1:1425936/71‑1 (MQ=255)
                                  gTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                    <  1:103155/70‑1 (MQ=255)
                                  gTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                    <  1:223323/70‑1 (MQ=255)
                                   ttCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCat                                                            >  1:3058890/1‑45 (MQ=255)
                                   ttCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCat                                                            >  1:3175454/1‑45 (MQ=255)
                                   ttCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTg                                              <  1:3360283/59‑1 (MQ=255)
                                    tCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAcc                                 >  1:2924367/1‑71 (MQ=255)
                                     cACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCa                                            >  1:3512929/1‑59 (MQ=255)
                                     cACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCa                                            >  1:2991074/1‑59 (MQ=255)
                                     cACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGaa                                   >  1:703325/1‑68 (MQ=255)
                                      aCTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCatt                                                           <  1:3298820/43‑1 (MQ=255)
                                       cTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                              >  1:1297977/1‑71 (MQ=255)
                                        tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATtattat                                                      <  1:463245/46‑1 (MQ=255)
                                        tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATtattat                                                      <  1:49422/46‑1 (MQ=255)
                                        tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGc                                             <  1:782099/55‑1 (MQ=255)
                                          tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAg                                           >  1:3582320/1‑55 (MQ=255)
                                          tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAc                           <  1:1873626/71‑1 (MQ=255)
                                           cGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                          >  1:3562444/1‑71 (MQ=255)
                                           cGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                          >  1:2009700/1‑71 (MQ=255)
                                           cGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                          >  1:126618/1‑71 (MQ=255)
                                           cGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                          >  1:2204132/1‑71 (MQ=255)
                                           cGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAAAGAACCTGCCAcc                          >  1:1288612/1‑71 (MQ=255)
                                               gACGGGTAAAGGCCACCACACCGATATCGCCattatt                                                        <  1:34199/37‑1 (MQ=255)
                                               gACGGGTAAAGGCCACCACACCGATATCGCCattatt                                                        <  1:1743639/37‑1 (MQ=255)
                                               gACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAAc                                      >  1:732090/1‑55 (MQ=255)
                                               gACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGt                        >  1:264949/1‑69 (MQ=255)
                                               gACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTg                       >  1:2103921/1‑70 (MQ=255)
                                               gACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTg                       >  1:2948669/1‑70 (MQ=255)
                                                aCGGGTAAAGGCCACCACACCGATATCGCCATTGTTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGa                     >  1:3119840/1‑71 (MQ=255)
                                                aCGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGt                                                  >  1:1969994/1‑42 (MQ=255)
                                                aCGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGt                                                  >  1:3210699/1‑42 (MQ=255)
                                                 cGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGat                    <  1:107777/71‑1 (MQ=255)
                                                  cggTAAAGGCCACCACACTGATATCGCTATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGata                   <  1:2913239/70‑1 (MQ=255)
                                                  cggTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGata                   <  1:1142375/70‑1 (MQ=255)
                                                     tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                    <  1:437630/51‑1 (MQ=255)
                                                     tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                    <  1:1634263/51‑1 (MQ=255)
                                                     tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                    <  1:3151603/51‑1 (MQ=255)
                                                     tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                    <  1:2626702/51‑1 (MQ=255)
                                                       aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAc                                  <  1:1327504/51‑1 (MQ=255)
                                                       aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa               >  1:101646/1‑70 (MQ=255)
                                                       aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa               >  1:3400458/1‑70 (MQ=255)
                                                         ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACg                                     <  1:2084206/46‑1 (MQ=255)
                                                         ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg            >  1:3491916/1‑71 (MQ=255)
                                                          gCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATc                 <  1:2324813/65‑1 (MQ=255)
                                                           ccaccaCACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGt           <  1:1497762/70‑1 (MQ=255)
                                                           ccaccaCACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTa          <  1:3130421/71‑1 (MQ=255)
                                                            caccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAt         <  1:3672272/71‑1 (MQ=255)
                                                              ccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAttn       >  1:2240281/1‑70 (MQ=255)
                                                              ccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTc       <  1:1595025/71‑1 (MQ=255)
                                                               cacaCCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                         >  1:1617022/1‑52 (MQ=255)
                                                               cacaCCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                         >  1:3231358/1‑52 (MQ=255)
                                                               cacaCCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTcc      <  1:2980058/71‑1 (MQ=255)
                                                                acacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCa                            >  1:1396039/1‑48 (MQ=255)
                                                                acacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCa                            >  1:2485113/1‑48 (MQ=255)
                                                                 cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg    >  1:2953309/1‑71 (MQ=255)
                                                                 cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg    >  1:2213973/1‑71 (MQ=255)
                                                                   ccGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGt  <  1:1163074/71‑1 (MQ=255)
                                                                    cGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                    <  1:1945981/36‑1 (MQ=255)
                                                                    cGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                    <  1:740471/36‑1 (MQ=255)
                                                                    cGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg    >  1:3532556/1‑68 (MQ=255)
                                                                    cGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg    >  1:1053755/1‑68 (MQ=255)
                                                                    |                                                                     
GATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGT  >  Exported/1200965‑1201102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑