BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,201,054	T→C	5.3%	G69G (GGT→GGC)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,201,054	0	T	C	5.3%	276.3 / 5.4	94	G69G (GGT→GGC)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand): ref base T (42/47); new base C (3/2); total (45/49)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 101 total aligned reads displayed.

CGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGC  >  Exported/1200985‑1201124

cGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTc                                                                       <  1:3279030/71‑1 (MQ=255)

 gttgACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCt                                                                      >  1:3328081/4‑71 (MQ=255)

 gttgACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCt                                                                      >  1:3112389/4‑71 (MQ=255)

    gACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTg                                                                    <  1:1827697/70‑1 (MQ=255)

    gACGTGTACGGCTCGCTCTCTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGc                                                                   >  1:3353179/1‑71 (MQ=255)

      cGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAg                                                                 >  1:3507445/1‑71 (MQ=255)

        tttATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCttc                                                                    <  1:3356120/66‑2 (MQ=255)

        tttATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGc                                                                   <  1:2731205/67‑1 (MQ=255)

        tttATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGt                                                               <  1:3514803/71‑1 (MQ=255)

            tGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAAc                                                            >  1:579977/1‑70 (MQ=255)

            tGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAAc                                                            >  1:3591813/1‑70 (MQ=255)

              gTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGTTAACGaa                                                         <  1:1425936/71‑1 (MQ=255)

              gTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                          <  1:103155/70‑1 (MQ=255)

              gTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                          <  1:223323/70‑1 (MQ=255)

               ttCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTg                                                                    <  1:3360283/59‑1 (MQ=255)

                tCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAcc                                                       >  1:2924367/1‑71 (MQ=255)

                 cACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCa                                                                  >  1:2991074/1‑59 (MQ=255)

                 cACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCa                                                                  >  1:3512929/1‑59 (MQ=255)

                 cACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGaa                                                         >  1:703325/1‑68 (MQ=255)

                   cTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                    >  1:1297977/1‑71 (MQ=255)

                    tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGc                                                                   <  1:782099/55‑1 (MQ=255)

                      tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAg                                                                 >  1:3582320/1‑55 (MQ=255)

                      tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAc                                                 <  1:1873626/71‑1 (MQ=255)

                       cGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                                                >  1:2204132/1‑71 (MQ=255)

                       cGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                                                >  1:126618/1‑71 (MQ=255)

                       cGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                                                >  1:2009700/1‑71 (MQ=255)

                       cGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                                                >  1:3562444/1‑71 (MQ=255)

                       cGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAAAGAACCTGCCAcc                                                >  1:1288612/1‑71 (MQ=255)

                           gACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAAc                                                            >  1:732090/1‑55 (MQ=255)

                           gACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGt                                              >  1:264949/1‑69 (MQ=255)

                           gACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTg                                             >  1:2948669/1‑70 (MQ=255)

                           gACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTg                                             >  1:2103921/1‑70 (MQ=255)

                            aCGGGTAAAGGCCACCACACCGATATCGCCATTGTTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGa                                           >  1:3119840/1‑71 (MQ=255)

                            aCGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGt                                                                        >  1:1969994/1‑42 (MQ=255)

                            aCGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGt                                                                        >  1:3210699/1‑42 (MQ=255)

                             cGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGat                                          <  1:107777/71‑1 (MQ=255)

                              cggTAAAGGCCACCACACTGATATCGCTATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGata                                         <  1:2913239/70‑1 (MQ=255)

                              cggTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGata                                         <  1:1142375/70‑1 (MQ=255)

                                 tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                          <  1:2626702/51‑1 (MQ=255)

                                 tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                          <  1:3151603/51‑1 (MQ=255)

                                 tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                          <  1:437630/51‑1 (MQ=255)

                                 tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                          <  1:1634263/51‑1 (MQ=255)

                                   aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAc                                                        <  1:1327504/51‑1 (MQ=255)

                                   aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                     >  1:3400458/1‑70 (MQ=255)

                                   aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                     >  1:101646/1‑70 (MQ=255)

                                     ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACg                                                           <  1:2084206/46‑1 (MQ=255)

                                     ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                  >  1:3491916/1‑71 (MQ=255)

                                      gCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATc                                       <  1:2324813/65‑1 (MQ=255)

                                       ccaccaCACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGt                                 <  1:1497762/70‑1 (MQ=255)

                                       ccaccaCACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTa                                <  1:3130421/71‑1 (MQ=255)

                                        caccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAt                               <  1:3672272/71‑1 (MQ=255)

                                          ccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAttn                             >  1:2240281/1‑70 (MQ=255)

                                          ccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTc                             <  1:1595025/71‑1 (MQ=255)

                                           cacaCCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                               >  1:1617022/1‑52 (MQ=255)

                                           cacaCCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                               >  1:3231358/1‑52 (MQ=255)

                                           cacaCCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTcc                            <  1:2980058/71‑1 (MQ=255)

                                            acacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCa                                                  >  1:1396039/1‑48 (MQ=255)

                                            acacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCa                                                  >  1:2485113/1‑48 (MQ=255)

                                             cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg                          >  1:2953309/1‑71 (MQ=255)

                                             cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg                          >  1:2213973/1‑71 (MQ=255)

                                               ccGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGt                        <  1:1163074/71‑1 (MQ=255)

                                                cGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                          <  1:1945981/36‑1 (MQ=255)

                                                cGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                          <  1:740471/36‑1 (MQ=255)

                                                cGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg                          >  1:1053755/1‑68 (MQ=255)

                                                cGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg                          >  1:3532556/1‑68 (MQ=255)

                                                  atatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGtttt                     <  1:2282105/71‑1 (MQ=255)

                                                  atatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGttt                      >  1:3084908/1‑70 (MQ=255)

                                                     tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCgg                         <  1:914420/64‑1 (MQ=255)

                                                     tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCgg                         <  1:3603853/64‑1 (MQ=255)

                                                     tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAtt                  <  1:753858/71‑1 (MQ=255)

                                                     tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                   <  1:3365994/70‑1 (MQ=255)

                                                      cGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                   <  1:2119030/69‑1 (MQ=255)

                                                       gCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcg                >  1:1857537/1‑71 (MQ=255)

                                                       gCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcg                >  1:196092/1‑71 (MQ=255)

                                                       gCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcg                >  1:3040736/1‑71 (MQ=255)

                                                       gCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTc                 <  1:1505837/70‑1 (MQ=255)

                                                       gCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTc                 <  1:2861/70‑1 (MQ=255)

                                                       gCCATTATTATGGGTCTTGCAGGTAACGAACCTGCAACCGTGGATATCGACAGTATTCCCGGTTTTATTcg                >  1:449137/1‑71 (MQ=255)

                                                        ccATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgc               <  1:571013/71‑1 (MQ=255)

                                                        ccATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgc               <  1:894222/71‑1 (MQ=255)

                                                         cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGt                        <  1:2257873/61‑1 (MQ=255)

                                                         cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGt                        <  1:2591161/61‑1 (MQ=255)

                                                           ttattaTGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                  <  1:1904939/49‑1 (MQ=255)

                                                           ttattaTGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                  <  1:950297/49‑1 (MQ=255)

                                                           ttattaTGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                  <  1:1279918/49‑1 (MQ=255)

                                                           ttattaTGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCgg                         <  1:3587700/58‑1 (MQ=255)

                                                           ttattaTGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGttt                      >  1:3620120/1‑61 (MQ=255)

                                                           ttattaTGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGAc            <  1:2611666/71‑1 (MQ=255)

                                                              ttatGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTc                             <  1:2512638/51‑1 (MQ=255)

                                                                atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag        >  1:3488654/1‑70 (MQ=255)

                                                                atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag        >  1:3099413/1‑70 (MQ=255)

                                                                  gggTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCgg                         >  1:1051382/1‑51 (MQ=255)

                                                                   ggTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTccc                           >  1:3315683/1‑48 (MQ=255)

                                                                   ggTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGa             >  1:3647710/1‑62 (MQ=255)

                                                                   ggTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaaga    <  1:2159592/71‑1 (MQ=255)

                                                                    gTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAagag   <  1:1346601/71‑1 (MQ=255)

                                                                     tCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAagag   >  1:2761975/1‑70 (MQ=255)

                                                                     tCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgc  >  1:39375/1‑71 (MQ=255)

                                                                     tCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgc  >  1:1984204/1‑71 (MQ=255)

                                                                     tCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgc  >  1:3412887/1‑71 (MQ=255)

CGTGGACGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGC  >  Exported/1200985‑1201124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`