Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,209,604 A→C 11.1% M1M (ATG→CTG) † yebN → conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,209,6040AC11.1% 192.1 / 22.4 81M1M (ATG→CTG) †yebNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (32/40);  new base C (4/5);  total (36/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAA  >  Exported/1209535‑1209674
                                                                     |                                                                      
gttgttCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCa                                                                        <  1:1686606/70‑1 (MQ=255)
    ttCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAat                                                                   <  1:3660069/71‑1 (MQ=255)
    ttCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAat                                                                   <  1:2207867/71‑1 (MQ=255)
       aTGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAatat                                                                 >  1:3393570/1‑70 (MQ=255)
       aTGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAatat                                                                 <  1:2441505/70‑1 (MQ=255)
       aTGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAatat                                                                 >  1:1909718/1‑70 (MQ=255)
       aTGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATc                                                                <  1:1420789/71‑1 (MQ=255)
       aTGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATc                                                                <  1:1416384/71‑1 (MQ=255)
          tATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGaa                                                                    >  1:1172031/1‑64 (MQ=255)
            tGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTg                                                            <  1:1365067/70‑1 (MQ=255)
            tGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGc                                                           <  1:3407111/71‑1 (MQ=255)
            tGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGc                                                           <  1:1950448/71‑1 (MQ=255)
            tGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGc                                                           <  1:2208625/71‑1 (MQ=255)
               aTGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTAc                                                        >  1:217582/1‑71 (MQ=255)
               aTGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTAc                                                        >  1:767951/1‑71 (MQ=255)
                tGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACt                                                       >  1:797423/1‑71 (MQ=255)
                  tttGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTg                                                      <  1:20805/70‑1 (MQ=255)
                  tttGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTg                                                      <  1:3446148/70‑1 (MQ=255)
                   ttGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGtt                                                    >  1:1202979/1‑71 (MQ=255)
                        gggggCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTtcttct                                               >  1:888717/1‑71 (MQ=255)
                        gggggCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTtcttct                                               >  1:3375316/1‑71 (MQ=255)
                         ggggCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGttcttc                                                <  1:1493471/69‑1 (MQ=255)
                              gatgatGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTa                                                         <  1:2445825/55‑1 (MQ=255)
                               atgatgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGttt                                        >  1:3195865/1‑71 (MQ=255)
                               atgatgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGttt                                        <  1:68203/71‑1 (MQ=255)
                               atgatgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGttt                                        <  1:1259177/71‑1 (MQ=255)
                                tgatgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTg                                       <  1:361226/71‑1 (MQ=255)
                                tgatgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTg                                       <  1:3656178/71‑1 (MQ=255)
                                tgatgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTg                                       <  1:138227/71‑1 (MQ=255)
                                  atgTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTg                                       <  1:2370317/69‑1 (MQ=255)
                                     tgtTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTAt                                   <  1:2804970/70‑1 (MQ=255)
                                       ttttATGGATACCCAGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTc                                <  1:662710/71‑1 (MQ=255)
                                        tttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTc                                >  1:113890/1‑70 (MQ=255)
                                         ttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTcttctt                                              >  1:1473765/1‑55 (MQ=255)
                                         ttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTcttctt                                              >  1:565169/1‑55 (MQ=255)
                                         ttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTg                                       >  1:346183/1‑62 (MQ=255)
                                         ttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCga                              >  1:2049963/1‑71 (MQ=255)
                                         ttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCga                              >  1:421567/1‑71 (MQ=255)
                                         ttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCga                              >  1:1676249/1‑71 (MQ=255)
                                         ttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCg                               >  1:417144/1‑70 (MQ=255)
                                         ttATGGATACCCCGGTCACGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCg                               <  1:3633222/70‑1 (MQ=255)
                                            tGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCgatgg                           <  1:1775100/71‑1 (MQ=255)
                                             ggATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCgatgg                           <  1:1225832/70‑1 (MQ=255)
                                               aTACCACGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGt                                     >  1:1855295/1‑58 (MQ=255)
                                                 aCCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCa                      >  1:2717337/1‑71 (MQ=255)
                                                  ccccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCAt                     >  1:2089003/1‑71 (MQ=255)
                                                  ccccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCAt                     >  1:696282/1‑71 (MQ=255)
                                                   cccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCAtt                    <  1:1530287/71‑1 (MQ=255)
                                                   cccGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCAtt                    <  1:2266527/71‑1 (MQ=255)
                                                     cGGTCAGGACATTGTCATGAATATCACTGCTACTg                                                      <  1:1949263/35‑1 (MQ=255)
                                                      ggTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgc                 >  1:3625119/1‑71 (MQ=255)
                                                      ggTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgc                 >  1:3620630/1‑71 (MQ=255)
                                                       gTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTgg                                      <  1:209172/49‑1 (MQ=255)
                                                        tCATGACAATGTCCTGAATATCACTGCTTCTGTTcttctt                                              <  1:2896973/40‑1 (MQ=21)
                                                        tCATGACAATGTCCTGAATATCACTGCTACTGTTcttctt                                              <  1:1522551/40‑1 (MQ=25)
                                                        tCATGACAATGTCCTGAATATCACTGCTACTGTTcttctt                                              <  1:3008382/40‑1 (MQ=25)
                                                        tCAGGACATTGTCATGAATATCACTGCTACTGttcttc                                                <  1:2887124/38‑1 (MQ=255)
                                                        tCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgctg               <  1:1016760/71‑1 (MQ=255)
                                                        tCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgctg               <  1:1228835/71‑1 (MQ=255)
                                                        tCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgctg               <  1:300759/71‑1 (MQ=255)
                                                        tCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgctg               <  1:1361025/71‑1 (MQ=255)
                                                        tCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgctg               <  1:1547098/71‑1 (MQ=255)
                                                         cAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTa                                    >  1:2954304/1‑49 (MQ=255)
                                                         cAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTa                                    >  1:1698920/1‑49 (MQ=255)
                                                          atgACAATGTCCTGAATATCACTGCTACTGTTCTTCTTGc                                            >  1:974135/3‑40 (MQ=37)
                                                          atgACAATGTCCTGAATATCACTGCTACTGTTCTTCTTGc                                            >  1:135230/3‑40 (MQ=37)
                                                          atgACAATGTCCTGAATATCACTGCTACTGTTCTTCTTGc                                            >  1:1533120/3‑40 (MQ=37)
                                                          atgACAATGTCCTGAATATCACTGCTACTGTTCTTCTTGc                                            >  1:1964630/3‑40 (MQ=37)
                                                          aGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTtgctgc              <  1:850048/70‑1 (MQ=255)
                                                          aGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCa             >  1:3467839/1‑71 (MQ=255)
                                                            gACAATGTCCTGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCa                      <  1:3064541/60‑1 (MQ=255)
                                                            gACAATGTCCTGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCa                      <  1:1484716/60‑1 (MQ=255)
                                                             aCATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCa          <  1:1119945/71‑1 (MQ=255)
                                                              cATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCaa         >  1:2651490/1‑71 (MQ=255)
                                                              cATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCaa         >  1:3287748/1‑71 (MQ=255)
                                                                ttGTCATGAATATCACTGCTACTGTTCTTCTTGCGttt                                        <  1:1977963/38‑1 (MQ=255)
                                                                ttGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGc                       <  1:2691765/55‑1 (MQ=255)
                                                                ttGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATc       <  1:2489315/71‑1 (MQ=255)
                                                                 tGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATc       <  1:1581985/70‑1 (MQ=255)
                                                                  gTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTa                                    >  1:559155/1‑40 (MQ=255)
                                                                   tCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCgat                             >  1:1467559/1‑46 (MQ=255)
                                                                   tCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCgat                             >  1:875383/1‑46 (MQ=255)
                                                                     aTGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCGTCAATCGGTa   >  1:1577210/1‑70 (MQ=255)
                                                                     aTGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTaa  <  1:1848745/71‑1 (MQ=255)
                                                                     aTGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTaa  <  1:7926/71‑1 (MQ=255)
                                                                     aTGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTaa  <  1:858148/71‑1 (MQ=255)
                                                                     aTGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTa   >  1:2579555/1‑70 (MQ=255)
                                                                     aTGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTa   >  1:2850230/1‑70 (MQ=255)
                                                                     |                                                                      
GTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAA  >  Exported/1209535‑1209674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: