Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,225,925	T→C	14.8%	L77P (CTT→CCT)	yebV →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,225,925	0	T	C	14.8%	137.5 / 14.3	54	L77P (CTT→CCT)	yebV	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/31);  new base C (6/2);  total (21/33)
Fisher's exact test for biased strand distribution p-value = 4.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTAAATGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTAAAATC  >  Exported/1225855‑1225994                                                                       |                                                                      cTAAATGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCt                                                                       >  1:3474011/1‑71 (MQ=255)  tAAATGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCtt                                                                      >  1:3040286/1‑71 (MQ=255)    aaTGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTg                                                                     <  1:874046/70‑1 (MQ=255)    aaTGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTg                                                                     <  1:3338052/70‑1 (MQ=255)    aaTGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGc                                                                    >  1:2024411/1‑71 (MQ=255)     aTGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGcc                                                                   <  1:2295809/71‑1 (MQ=255)        gCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTg                                                                 <  1:181/70‑1 (MQ=255)          gAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATc                                                              >  1:2715092/1‑71 (MQ=255)             cacTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCaa                                                           <  1:3418759/71‑1 (MQ=255)             cacTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCaa                                                           <  1:2283314/71‑1 (MQ=255)              acTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                          <  1:61175/71‑1 (MQ=255)                tctGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCaaa                                                          >  1:1480829/1‑69 (MQ=255)                  tGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAg                                                       >  1:2872002/1‑70 (MQ=255)                    tcatttACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAg                                                       <  1:1334424/65‑1 (MQ=255)                    tCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAg                                                       <  1:110900/68‑1 (MQ=255)                    tCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAg                                                       <  1:1502907/68‑1 (MQ=255)                    tCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGaa                                                     <  1:2373028/70‑1 (MQ=255)                    tCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGa                                                      <  1:1418959/69‑1 (MQ=255)                    tCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGGCTTGCCTGATCCAAAAAGa                                                      <  1:2875825/69‑1 (MQ=255)                      cTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAAccc                                                  <  1:609532/71‑1 (MQ=255)                         tACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGaa                                                     >  1:1110278/1‑65 (MQ=255)                         tACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCg                                                 <  1:1142297/69‑1 (MQ=255)                         tACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCg                                                 <  1:2881532/69‑1 (MQ=255)                         tACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCg                                                 <  1:1833419/69‑1 (MQ=255)                         tACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCGTc                                               >  1:1731088/1‑71 (MQ=255)                          aCCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTc                                               <  1:3577533/70‑1 (MQ=255)                          aCCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTc                                               <  1:2341315/70‑1 (MQ=255)                           ccGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCg                                                 <  1:2581567/67‑1 (MQ=255)                              tACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGc                                                                    >  1:2966936/1‑45 (MQ=255)                               aCCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATg                                         >  1:1673920/1‑71 (MQ=255)                                cccGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATgg                                        <  1:515164/71‑1 (MQ=255)                                  cGTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCGTCGGCATGGCg                                      >  1:3375109/1‑71 (MQ=255)                                   gTTACGATCAACAATCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCGTCGGCATGGCgg                                     >  1:576046/1‑71 (MQ=255)                                    ttACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGt                                                <  1:1583163/59‑1 (MQ=255)                                      aCGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt                                   >  1:1023781/1‑70 (MQ=255)                                           caacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt                                   <  1:1108341/65‑1 (MQ=255)                                           caacaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGt                                   <  1:1962182/65‑1 (MQ=255)                                           caacaaTCTGATGCCTGGATTATGCGTCCTGCCTGATCCAAAAAGAACCCGTCGGCa                                           >  1:2154582/1‑57 (MQ=255)                                             acaaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTa                                 >  1:3316218/1‑65 (MQ=255)                                              caaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCa                                           >  1:1156683/1‑54 (MQ=255)                                              caaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTg                             >  1:294201/1‑68 (MQ=255)                                               aaTCTGATGCCTGGATTATGCGTCTTGCCTGATcc                                                             <  1:1353067/35‑1 (MQ=255)                                               aaTCTGATGCCTGGATTATGCGTCTTGCCTGATcc                                                             <  1:2909116/35‑1 (MQ=255)                                               aaTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCt                         <  1:1558804/71‑1 (MQ=255)                                                aTCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTg                        <  1:2548508/71‑1 (MQ=255)                                                          tGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTtccc              >  1:2933162/1‑71 (MQ=255)                                                          tGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTtccc              >  1:1153934/1‑71 (MQ=255)                                                            gATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGTATGGCGGGTTATTTGTCCt                         >  1:3270053/1‑58 (MQ=255)                                                             aTTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCt                         >  1:1622048/1‑57 (MQ=255)                                                                 tGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGt        >  1:3458514/1‑70 (MQ=255)                                                                 tGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGt        <  1:2510233/70‑1 (MQ=255)                                                                   cGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTaa      <  1:2146781/70‑1 (MQ=255)                                                                   cGTCTTGCCTGATCCAAAAAGAACCCATCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTaaa     <  1:3339591/71‑1 (MQ=255)                                                                    gTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTa                    <  1:1014100/55‑1 (MQ=255)                                                                    gTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTa                    <  1:769590/55‑1 (MQ=255)                                                                      cTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTAAAAtc  >  1:1812337/1‑71 (MQ=255)                                                                       ttGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTa                    >  1:83440/1‑52 (MQ=255)                                                                       ttGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTa                    >  1:1183737/1‑52 (MQ=255)                                                                       |                                                                      CTAAATGGCGAACACTCTGTCCTTTACCGTACCCGTTACGATCAACAATCTGATGCCTGGATTATGCGTCTTGCCTGATCCAAAAAGAACCCGTCGGCATGGCGGGTTATTTGTCCTGGTTATTCCCCCGTTGTAAAATC  >  Exported/1225855‑1225994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑