| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,266,778 | C→T | 9.4% | G210D (GGT→GAT) | yecA ← | conserved metal‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,266,778 | 0 | C | T | 9.4% | 224.3 / 9.8 | 74 | G210D (GGT→GAT) | yecA | conserved metal‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (36/31); new base T (3/4); total (39/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTG > Exported/1266708‑1266848 | gCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTAc > 1:1933068/1‑71 (MQ=255) aGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACTACTACCGc < 1:514058/71‑1 (MQ=255) gAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCa > 1:1911662/1‑71 (MQ=255) cTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAgg < 1:318452/71‑1 (MQ=255) cTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTCACCACTACCGCACGGGCAAgg < 1:1730198/71‑1 (MQ=255) tGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAgg < 1:3027059/70‑1 (MQ=255) tGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGa > 1:2012319/1‑71 (MQ=255) cacCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCg < 1:1686947/71‑1 (MQ=255) cacCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCg > 1:3274118/1‑71 (MQ=255) cccAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGt > 1:2400259/1‑70 (MQ=255) aaTTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACg > 1:3432368/1‑71 (MQ=255) tGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACTGGCAAGGATCGTTACGccc < 1:857535/70‑1 (MQ=255) ggCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGccc < 1:1259937/69‑1 (MQ=255) ccTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGt < 1:3066458/71‑1 (MQ=255) ccTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGt < 1:3712404/71‑1 (MQ=255) ccTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGt < 1:3200679/71‑1 (MQ=255) tctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTa > 1:1951474/1‑60 (MQ=255) tctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTa > 1:2244835/1‑60 (MQ=255) tctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTa > 1:2366566/1‑60 (MQ=255) tctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTa > 1:518507/1‑60 (MQ=255) tctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTa > 1:3266123/1‑60 (MQ=255) tctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGttt > 1:603475/1‑71 (MQ=255) tctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGttt > 1:2022214/1‑71 (MQ=255) tctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCACGGttt > 1:1293614/1‑71 (MQ=255) ctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAt > 1:1252007/1‑54 (MQ=255) ctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAt > 1:2999531/1‑54 (MQ=255) ctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAt > 1:2810043/1‑54 (MQ=255) ctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAt > 1:84585/1‑54 (MQ=255) ctTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGAt > 1:3175280/1‑54 (MQ=255) ttAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGt > 1:3324479/1‑56 (MQ=255) ttAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGt > 1:2538205/1‑56 (MQ=255) ttAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGt > 1:1200615/1‑56 (MQ=255) ttAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGttt < 1:3589241/69‑1 (MQ=255) ttAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGttt < 1:2010021/69‑1 (MQ=255) ttAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGttt < 1:1884082/69‑1 (MQ=255) ttAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtct > 1:1694940/1‑71 (MQ=255) aaTGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtctc < 1:317897/70‑1 (MQ=255) aaTGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtctc < 1:499986/70‑1 (MQ=255) aTGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtc < 1:2461561/67‑1 (MQ=255) aTGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTctct < 1:2238057/70‑1 (MQ=255) tGCAGGCAGCACTGCTTAAATTTCTTACCACTATCGCa < 1:3606391/38‑1 (MQ=255) tGCAGGCAGCACTGCTTAAATTTCTTACCACTATCGCa < 1:1385962/38‑1 (MQ=255) tGCAGGCAGCACTGCTTAAATTTCTTACCACTATCGCa < 1:2707910/38‑1 (MQ=255) gcaggcagCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCg < 1:16407/60‑1 (MQ=255) gcaggcagCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCg < 1:1970342/60‑1 (MQ=255) caggcagCACTGCTTAAATTTCTTACCACTATCGCa < 1:2764768/36‑1 (MQ=255) caggcagCACTGCTTAAATTTCTTACCACTATCGCACg > 1:688083/1‑38 (MQ=255) caggcagCACTGCTTAAATTTCTTACCACTATCGCACg > 1:2128669/1‑38 (MQ=255) caggcagCACTGCTTAAATTTCTTACCACTATCGCACg > 1:1076602/1‑38 (MQ=255) aggcagCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGt > 1:3136093/1‑60 (MQ=255) agcaCTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCtt > 1:3447936/1‑69 (MQ=255) caCTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGt > 1:1504867/1‑43 (MQ=255) caCTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGt > 1:757404/1‑43 (MQ=255) caCTGCTTAAATTTCTTACCACTACCGCACGGGAAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGAt > 1:2798755/1‑71 (MQ=255) aCTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATc < 1:3389719/71‑1 (MQ=255) cTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg > 1:891074/1‑71 (MQ=255) tGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGttt < 1:3725448/53‑1 (MQ=255) tGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCgg > 1:464066/1‑71 (MQ=255) cTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTtct > 1:220148/1‑53 (MQ=255) cTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCgg < 1:960045/69‑1 (MQ=255) cTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCgg < 1:639293/69‑1 (MQ=255) cTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGt < 1:2806333/70‑1 (MQ=255) aaTTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg < 1:3678801/71‑1 (MQ=255) aaTTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg < 1:1233860/71‑1 (MQ=255) aaTTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg < 1:1238307/71‑1 (MQ=255) aaTTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTg < 1:1253878/71‑1 (MQ=255) tCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGaa > 1:1314791/1‑69 (MQ=255) cTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg > 1:3712933/1‑59 (MQ=255) cTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg > 1:95232/1‑59 (MQ=255) cTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCg > 1:1840154/1‑59 (MQ=255) ttACCACTACCGCACGGGCAATGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATAGGTTGCTg < 1:3325209/65‑1 (MQ=255) aCCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAg > 1:1595991/1‑68 (MQ=255) cACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt < 1:1081662/71‑1 (MQ=255) cACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCtttt < 1:2001286/71‑1 (MQ=255) tACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc > 1:2694939/1‑71 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCTGTTGCTGAACAGCTTTTTcc < 1:2220623/69‑1 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc < 1:1651541/69‑1 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTcc < 1:2529648/69‑1 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTg > 1:2462450/1‑71 (MQ=255) ccGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTg > 1:373903/1‑71 (MQ=255) | GCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAGGCAGCACTGCTTAAATTTCTTACCACTACCGCACGGGCAAGGATCGTTACGCCCCGGTTTCTCTTCTGCTTTGATCGGTTGCTGAACAGCTTTTTCCTG > Exported/1266708‑1266848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |