| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,302,608 | T→C | 57.1% | K158R (AAG→AGG) | dacD ← | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,302,608 | 0 | T | C | 57.1% | 59.2 / 151.3 | 119 | K158R (AAG→AGG) | dacD | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base T (27/24); new base C (27/41); total (54/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
CCGAGCTATGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAG > Exported/1302540‑1302674 | cGAGCTATGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCctct < 1:2182264/71‑1 (MQ=255) cGAGCTATGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCctct < 1:1557524/71‑1 (MQ=255) aGCTATGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCg > 1:617730/1‑71 (MQ=255) gCTATGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCg < 1:2189956/70‑1 (MQ=255) aTGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCa > 1:825375/1‑70 (MQ=255) aTGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCa > 1:56948/1‑70 (MQ=255) ggCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGc > 1:1520830/1‑64 (MQ=255) ggCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGc > 1:3471469/1‑64 (MQ=255) ggCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGc > 1:2938910/1‑64 (MQ=255) ggCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCAt > 1:2259044/1‑66 (MQ=255) gCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAg < 1:2697169/67‑1 (MQ=255) gCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAg < 1:2436242/67‑1 (MQ=255) gCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAg < 1:3334635/67‑1 (MQ=255) gCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAg < 1:1909612/67‑1 (MQ=255) gCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAg < 1:2204596/67‑1 (MQ=255) gCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAg < 1:2353440/67‑1 (MQ=255) cAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGc > 1:615637/1‑61 (MQ=255) cAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAgtt > 1:2598460/1‑67 (MQ=255) cATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCAtt < 1:1372523/71‑1 (MQ=255) cATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCAtt < 1:3423641/71‑1 (MQ=255) tCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGTGATGCAGCTTCTCGGCATAg < 1:941405/57‑1 (MQ=255) tCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAg < 1:2869200/57‑1 (MQ=255) ccAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTc > 1:2305511/1‑70 (MQ=255) cAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTca < 1:3322182/70‑1 (MQ=255) cAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCtctc > 1:1046427/1‑48 (MQ=255) aGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTAGGCATAGTTGTTCATCAtt > 1:3154702/1‑66 (MQ=255) aGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTcaa > 1:2177228/1‑70 (MQ=255) aGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTca > 1:25286/1‑69 (MQ=255) gACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTcaaca < 1:1781591/71‑1 (MQ=255) gACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTcaac > 1:3452317/1‑70 (MQ=255) gACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTcaac > 1:2889328/1‑70 (MQ=255) aCCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTcaacaa < 1:2553424/71‑1 (MQ=255) aCCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTcaacaa < 1:2842602/71‑1 (MQ=255) aCCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTcaacaa < 1:729636/71‑1 (MQ=255) aTGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCgg < 1:3519511/44‑1 (MQ=255) cACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGc < 1:3505518/70‑1 (MQ=255) cACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGc < 1:1811226/70‑1 (MQ=255) cACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGc < 1:1046343/70‑1 (MQ=255) cACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGc < 1:2569473/70‑1 (MQ=255) cACTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGc < 1:247691/70‑1 (MQ=255) cTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGt < 1:3633801/71‑1 (MQ=255) cTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGt < 1:2798185/71‑1 (MQ=255) cTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGt < 1:1175391/71‑1 (MQ=255) cTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGt < 1:1339990/71‑1 (MQ=255) cTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGt < 1:2483759/71‑1 (MQ=255) cTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGt < 1:3046361/71‑1 (MQ=255) cTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGt < 1:1777534/71‑1 (MQ=255) cTGTTTCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGt < 1:3554347/71‑1 (MQ=255) tGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGtt > 1:91098/1‑71 (MQ=255) tttCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGc < 1:3334875/71‑1 (MQ=255) tttCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGc > 1:3713419/1‑71 (MQ=255) tttCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGc > 1:2811512/1‑71 (MQ=255) ttCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAg > 1:1420998/1‑40 (MQ=255) ttCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAg > 1:3350712/1‑40 (MQ=255) tCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTcaaca < 1:2350537/57‑1 (MQ=255) tCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTcaaca < 1:1326320/57‑1 (MQ=255) tCAAAATGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTcaaca > 1:1152912/1‑57 (MQ=255) aaaaTGCGTATCCTTGAGATGCAGCCTCTCGGCATAgttgtt > 1:339093/1‑42 (MQ=255) aaaaTGCGTATCCTTGAGATGCAGCCTCTCGGCATAgttgtt > 1:1924425/1‑42 (MQ=255) aaaaTGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCAc > 1:2533232/1‑71 (MQ=255) aaaTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGccc < 1:2681693/68‑1 (MQ=255) aaTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCg < 1:2583769/71‑1 (MQ=255) aaTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCg < 1:427153/71‑1 (MQ=255) aaTGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTtcatc < 1:3543510/44‑1 (MQ=255) aaTGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTtcatc < 1:2671851/44‑1 (MQ=255) aaTGCGTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCg < 1:1858577/71‑1 (MQ=255) aTGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCAc > 1:1650457/1‑68 (MQ=255) aTGCGTATCCTTGAGATGCAGCCTCTCGGCATAgttgtt > 1:3208250/1‑39 (MQ=255) aTGCGTATCCTTGAGATGCAGCCTCTCGGCATAgttgtt > 1:1392856/1‑39 (MQ=255) aTGCGTATCCTTGAGATGCAGCCTCTCGGCATAgttgtt > 1:576713/1‑39 (MQ=255) aTGCGTATCCTTGAGATGCAGCCTCTCGGCATAgttgtt > 1:2740245/1‑39 (MQ=255) aTGAGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCa > 1:2023985/1‑67 (MQ=255) gTATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAat < 1:1063571/71‑1 (MQ=255) tATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTcaacaa < 1:99223/48‑1 (MQ=255) tATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTcaacaa < 1:2296465/48‑1 (MQ=255) tATCCTTGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTcaacaa < 1:2524372/48‑1 (MQ=255) tCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTcaac > 1:1840466/1‑44 (MQ=255) tCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAAc > 1:89559/1‑48 (MQ=255) tCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACt > 1:1569856/1‑49 (MQ=255) cTTGTGATGCAGCTTCTCGGCATAGTTGTTCATCATTTcaacaa > 1:2842967/1‑44 (MQ=255) cTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGt < 1:2290947/71‑1 (MQ=255) ttGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAAc < 1:3215493/45‑1 (MQ=255) tGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGt > 1:3335406/1‑69 (MQ=255) tGAGATGCAGCTTCTCGGCATAGATGTTCATCATTTCAACAAAc > 1:3657164/1‑44 (MQ=255) tGAGATGCAGCCTCTCGGCATAGTTGTTCATCATTTc < 1:1630411/37‑1 (MQ=255) agaTGCAGCCTCTCGGCATAGTTGTTCATCATTTcaaca < 1:3122721/39‑1 (MQ=255) agaTGCAGCCTCTCGGCATAGTTGTTCATCATTTcaaca < 1:1187555/39‑1 (MQ=255) agaTGCAGCCTCTCGGCATAGTTGTTCATCATTTcaaca < 1:20692/39‑1 (MQ=255) agaTGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAg > 1:2922133/1‑70 (MQ=255) agaTGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGc < 1:686534/71‑1 (MQ=255) aTGCAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCa < 1:1501618/71‑1 (MQ=255) tGCAGCCTCTCGGCATAGTTGTTCATCATTTcaaca < 1:1673899/36‑1 (MQ=255) tGCAGCCTCTCGGCATAGTTGTTCATCATTTcaaca < 1:754717/36‑1 (MQ=255) tGCAGCCTCTCGGCATAGTTGTTCATCATTTcaaca < 1:2681552/36‑1 (MQ=255) cAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAAc > 1:436875/1‑37 (MQ=255) cAGCCTCTCGGCATAGTTGTTCATCATTTCAACAAAc > 1:1130017/1‑37 (MQ=255) aGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAata > 1:3244777/1‑57 (MQ=255) cTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGccc < 1:2255937/45‑1 (MQ=255) cTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGccc < 1:2290717/45‑1 (MQ=255) ttCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCagag < 1:554221/67‑1 (MQ=255) | CCGAGCTATGCTGGCCAGGTGCATCCAGACCATGCACTGTTTCAAAATGCGTATCCTTGAGATGCAGCTTCTCGGCATAGTTGTTCATCATTTCAACAAACTGCCGTTGCCCACCGGCAATATAGTCAGCCAGAG > Exported/1302540‑1302674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |