Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,362,418 A→G 5.9% intergenic (‑39/‑93) mrp ← / → metG antiporter inner membrane protein/methionyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,362,4180AG 5.9% 299.6 / 7.8 101intergenic (‑39/‑93)mrp/metGantiporter inner membrane protein/methionyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (46/49);  new base G (3/3);  total (49/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 107 total aligned reads displayed.

GTGATTTGGCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATCTAACCCCTTTTC  >  Exported/1362352‑1362487
                                                                  |                                                                     
gTGATTTGGCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGttt                                                                   <  1:1308471/71‑1 (MQ=255)
gTGATTTGGCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGt                                                                     <  1:1494749/69‑1 (MQ=255)
 tGATTTGGCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTa                                                                  >  1:989718/1‑71 (MQ=255)
   aTTTGGCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTAc                                                                 <  1:2292798/70‑1 (MQ=255)
    tttGGCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACTATCAGCTGATGGTTTATCt                                                               <  1:1240427/71‑1 (MQ=38)
      tGGCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTg                                                              >  1:680829/1‑70 (MQ=255)
       ggCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTga                                                             <  1:536263/70‑1 (MQ=255)
       ggCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTg                                                              >  1:1114221/1‑69 (MQ=255)
        gCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTTCCTgaa                                                            <  1:3724011/70‑1 (MQ=255)
        gCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTgaag                                                           <  1:2957832/71‑1 (MQ=255)
             ggATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAat                                                        <  1:1315448/69‑1 (MQ=255)
              gtttGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACGATCAGCTGATAGTTTACCTgaaga                                                          <  1:20327/64‑1 (MQ=255)
                 tGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAAtata                                                     >  1:934543/1‑68 (MQ=255)
                    tcgttcATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAg                                               >  1:2534982/1‑71 (MQ=255)
                       ttcATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGtac                                            >  1:1734717/1‑71 (MQ=255)
                       ttcATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGtac                                            >  1:2733916/1‑71 (MQ=255)
                       ttcATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGtac                                            <  1:2311476/71‑1 (MQ=255)
                         cATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGtact                                           >  1:2300195/1‑70 (MQ=255)
                         cATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGtact                                           >  1:2456184/1‑70 (MQ=255)
                           tCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCt                                                               <  1:2584561/48‑1 (MQ=255)
                            cccGATTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATagag                                                  >  1:505879/1‑60 (MQ=255)
                            cccGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATagag                                                  >  1:25862/1‑60 (MQ=255)
                            cccGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATagag                                                  >  1:3231713/1‑60 (MQ=255)
                            cccGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGtacttact                                       >  1:3212023/1‑71 (MQ=255)
                             ccGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGtacttact                                       <  1:2424689/70‑1 (MQ=255)
                             ccGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGtacttact                                       <  1:2769040/70‑1 (MQ=255)
                              cGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTActtactta                                     <  1:325019/71‑1 (MQ=255)
                              cGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTActtactta                                     <  1:2106392/71‑1 (MQ=255)
                                 cTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTAcc                                                                <  1:1233304/41‑1 (MQ=255)
                                 cTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTAcc                                                                <  1:1123310/41‑1 (MQ=255)
                                 cTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTaa                                    <  1:2939267/69‑1 (MQ=255)
                                     ttttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAtt                                <  1:227624/69‑1 (MQ=255)
                                     ttttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAtt                                <  1:435255/69‑1 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTgaagaa                                                         >  1:1134338/1‑42 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAtttt                              <  1:3017868/69‑1 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAtttt                              <  1:1674054/69‑1 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAt                                 <  1:438311/66‑1 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTcc                            >  1:3151622/1‑71 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTcc                            >  1:3205798/1‑71 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTcc                            >  1:3421195/1‑71 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTcc                            >  1:1529709/1‑71 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTc                             >  1:3530445/1‑70 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTc                             >  1:1046480/1‑70 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTc                             >  1:158982/1‑70 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTc                             <  1:1446998/70‑1 (MQ=255)
                                       ttttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTc                             >  1:2215406/1‑70 (MQ=255)
                                        tttGTATAGATAAACCATCAGCTGATAGTTTACCTgaa                                                            >  1:261210/1‑38 (MQ=255)
                                         ttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAttt                               >  1:3165101/1‑66 (MQ=255)
                                         ttGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAttt                               >  1:733850/1‑66 (MQ=255)
                                           gTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCAt                         <  1:2510063/70‑1 (MQ=255)
                                           gTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCAt                         <  1:3123049/70‑1 (MQ=255)
                                           gTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCAt                         <  1:668596/70‑1 (MQ=255)
                                           gTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCAt                         <  1:2164859/70‑1 (MQ=255)
                                           gTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCAt                         <  1:844195/70‑1 (MQ=255)
                                           gTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCAt                         <  1:767919/70‑1 (MQ=255)
                                            tataGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTActtactta                                     <  1:3274028/57‑1 (MQ=255)
                                            tataGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTActtactta                                     <  1:697024/57‑1 (MQ=255)
                                              taGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTgg                     <  1:1436616/71‑1 (MQ=255)
                                               aGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTccga                          >  1:424787/1‑63 (MQ=255)
                                               aGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTccc                           >  1:647512/1‑64 (MQ=255)
                                               aGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTgg                     >  1:3554033/1‑70 (MQ=255)
                                                gATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGaa                                                >  1:268172/1‑42 (MQ=255)
                                                 gtAAACTATCAGCTGATGGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCAtt                        <  1:3396541/64‑1 (MQ=38)
                                                 gtAAACTATCAGCTGATGGTTTACCTGAAGAAAATAGAGAAGTACTTACTTAACATTTTCCCAtt                        <  1:886247/64‑1 (MQ=37)
                                                  tAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTAc                  >  1:2283152/1‑70 (MQ=255)
                                                  tAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACt                 <  1:879229/71‑1 (MQ=255)
                                                   aaaCCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTa                >  1:189570/1‑71 (MQ=255)
                                                   aaaCCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTa                >  1:3590808/1‑71 (MQ=255)
                                                   aaaCCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTAATTAACATTTTc                             >  1:1159784/1‑58 (MQ=255)
                                                    aaCCATCAGCTGATAGTTTACCTGAAGAATATAGAGaa                                                >  1:3687740/1‑38 (MQ=255)
                                                    aaCCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTa                <  1:1229285/70‑1 (MQ=255)
                                                    aaCCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTa                <  1:2238198/70‑1 (MQ=255)
                                                    aaCCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTAt               >  1:1554592/1‑71 (MQ=255)
                                                     aCCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATc              >  1:1254277/1‑71 (MQ=255)
                                                     aCCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATc              <  1:3476155/71‑1 (MQ=255)
                                                      ctaTCAGCTGATGGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATc              >  1:2234617/3‑70 (MQ=255)
                                                      ctaTCAGCTGATGGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATc              >  1:1122395/3‑70 (MQ=255)
                                                      ctaTCAGCTGATGGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATc              >  1:3114279/3‑70 (MQ=255)
                                                      ccATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATc              >  1:3170391/1‑70 (MQ=255)
                                                      ccATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTCAACATTTTCCCATTTGGTACTATc              >  1:869977/1‑70 (MQ=255)
                                                       cATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATCTa            <  1:81874/71‑1 (MQ=255)
                                                           aGCTGATAGTTTACCTGTAGAATATAGAGAAGTACTTACTTAAGATTTTCCCATTTGGTACTATCTAAcc         <  1:3729958/70‑1 (MQ=255)
                                                           aGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAtt                                <  1:2737051/47‑1 (MQ=255)
                                                            gCTGATAGTTTACCTGAAGAATATAGATAAGTACTTACTTAACATTTTCCCAtt                        <  1:1484148/54‑1 (MQ=255)
                                                            gCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAtt                                <  1:3222488/46‑1 (MQ=255)
                                                             cTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAtttt                              >  1:2267784/1‑47 (MQ=255)
                                                             cTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAtttt                              >  1:3029730/1‑47 (MQ=255)
                                                             cTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAtttt                              >  1:2795740/1‑47 (MQ=255)
                                                                aTAGTTTACCTGAAGAATATAGAGAAGTActtactta                                     <  1:1871173/37‑1 (MQ=255)
                                                                aTAGTTTACCTGAAGAATATAGAGAAGTActtactta                                     <  1:1669465/37‑1 (MQ=255)
                                                                aTAGTTTACCTGAAGAATATAGAGAAGTActtactta                                     <  1:1654897/37‑1 (MQ=255)
                                                                aTAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATCTAACCCCtttt   <  1:2708827/71‑1 (MQ=255)
                                                                 tAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATCTAACCCCtttt   >  1:3031558/1‑70 (MQ=255)
                                                                 tAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATCTAACCCCTTTTc  <  1:2098131/71‑1 (MQ=255)
                                                                  aGTTTACCTGAAGAATATAGAGAAGTACTTACTTAAc                                   >  1:2417861/1‑37 (MQ=255)
                                                                  aGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAt                                 <  1:2899753/39‑1 (MQ=255)
                                                                  aGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAt                                 <  1:1426880/39‑1 (MQ=255)
                                                                  aGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACAt                                 <  1:3219870/39‑1 (MQ=255)
                                                                  aGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCAt                         <  1:788619/47‑1 (MQ=255)
                                                                  aGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCAt                         <  1:3690137/47‑1 (MQ=255)
                                                                  |                                                                     
GTGATTTGGCCTGGGATTGTTCGTTCATCCCGACTCCTTTTTTGTATAGATAAACCATCAGCTGATAGTTTACCTGAAGAATATAGAGAAGTACTTACTTAACATTTTCCCATTTGGTACTATCTAACCCCTTTTC  >  Exported/1362352‑1362487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: