| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,417,054 | A→G | 14.1% | Y416C (TAT→TGT) | yejM → | predicted hydrolase, inner membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,417,054 | 0 | A | G | 14.1% | 165.5 / 18.0 | 64 | Y416C (TAT→TGT) | yejM | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base A (37/18); new base G (4/5); total (41/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GTTCTCGTGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGATTCTGGCAAA > Exported/1416986‑1417124 | gTTCTCGTGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAata > 1:1195119/1‑69 (MQ=255) ctcGTGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGcc > 1:2548237/1‑71 (MQ=255) cGTGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCgg > 1:678184/1‑71 (MQ=255) gTGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCggg > 1:3658127/1‑71 (MQ=255) tGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAatat < 1:2277805/63‑1 (MQ=255) tGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAatat < 1:2441275/63‑1 (MQ=255) tGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAatat < 1:1620695/63‑1 (MQ=255) gggTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGcc < 1:2394460/66‑1 (MQ=255) gggTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGcc < 1:1569953/66‑1 (MQ=255) ggTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGggcg < 1:2780878/70‑1 (MQ=255) gTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGggcggc < 1:3126101/71‑1 (MQ=255) gTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGggcg > 1:398475/1‑69 (MQ=255) tttctttcAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATGTAGCCGGGCggca < 1:2223785/71‑1 (MQ=255) tctttcAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATGTAGCCGGGCggcagg > 1:256091/1‑71 (MQ=255) tctttcAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggcagg > 1:3475184/1‑71 (MQ=255) cAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAAt < 1:3541877/70‑1 (MQ=255) cAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAAt > 1:1640135/1‑70 (MQ=255) aaTGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGt > 1:3446790/1‑71 (MQ=255) aaTGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGt > 1:1003008/1‑71 (MQ=255) aTGTTAGTACCATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTc < 1:3419839/71‑1 (MQ=255) aTGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTc < 1:3552544/71‑1 (MQ=255) tGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATGTAGCCGGGCGGCAGGCAATGTCg < 1:1627305/71‑1 (MQ=255) tGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTc > 1:1129485/1‑70 (MQ=255) gTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGAt < 1:3148883/71‑1 (MQ=255) tACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATg < 1:1981291/71‑1 (MQ=255) aCATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGAc > 1:2856818/1‑68 (MQ=255) aTTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGAc > 1:341784/1‑66 (MQ=255) aTTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGAc > 1:318113/1‑66 (MQ=255) tGACGACAGCAATCAGCCGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATc > 1:2447754/1‑70 (MQ=255) tGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATc > 1:1604536/1‑70 (MQ=255) gacgacAGCAATCAGCAGGCATTTGCACGGAAATGTAGc > 1:456257/1‑39 (MQ=255) gacgacAGCAATCAGCAGGCATTTGCACGGAAATGTAGc > 1:3120405/1‑39 (MQ=255) gacgacAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggcaggc > 1:2387287/1‑51 (MQ=255) gacgacAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggcaggc > 1:2580858/1‑51 (MQ=255) gacgacAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggcaggc > 1:3227209/1‑51 (MQ=255) gacgacAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggcaggc > 1:1931701/1‑51 (MQ=255) gacgacAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggcaggc > 1:2949321/1‑51 (MQ=255) cgacAGCAATCAGCAGGCATTTGCACGGAAATGTAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAAcc < 1:3123868/71‑1 (MQ=255) gacAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATg < 1:2895456/58‑1 (MQ=255) gacAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATg < 1:2911668/58‑1 (MQ=255) gacAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACcg < 1:776546/71‑1 (MQ=255) cAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGAc > 1:640518/1‑58 (MQ=255) cAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACcgcg < 1:3639550/71‑1 (MQ=255) cAGCAATCAGCAGGCAGTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACcgcg > 1:302120/1‑71 (MQ=255) cAATCAGCAGGCATTTGCACGGAAATGTAGCCGGGCGGCAGGCAATGTCGATGACCAGATc > 1:222869/1‑61 (MQ=255) cAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGc > 1:753547/1‑71 (MQ=255) cAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGc > 1:2561748/1‑71 (MQ=255) tCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGa > 1:1461176/1‑48 (MQ=255) tCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGt < 1:2323854/66‑1 (MQ=255) tCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCt > 1:401017/1‑69 (MQ=255) gcagGCATTTGCACGGAAATGTAGCCGGGCGGCAGGCAATGTCGATGACCAg < 1:2219347/52‑1 (MQ=255) cATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTg < 1:1968236/71‑1 (MQ=255) aTTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGc > 1:1461387/1‑71 (MQ=255) ttGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGt > 1:3105810/1‑71 (MQ=255) ttGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGt > 1:3687935/1‑71 (MQ=255) tGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGt < 1:1558312/70‑1 (MQ=255) cACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCa > 1:3523959/1‑40 (MQ=255) cACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCa > 1:394529/1‑40 (MQ=255) aCGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGAtt > 1:1543679/1‑71 (MQ=255) aCGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGAtt > 1:30939/1‑71 (MQ=255) aCGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGAtt > 1:194177/1‑71 (MQ=255) aCGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGAtt > 1:3567832/1‑71 (MQ=255) aCGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGAtt > 1:1605780/1‑71 (MQ=255) aCGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGAtt > 1:3336303/1‑71 (MQ=255) cGGAAATGTAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGATTc < 1:3526236/71‑1 (MQ=255) cGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGATTc < 1:403774/71‑1 (MQ=255) gAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGc > 1:1766080/1‑50 (MQ=255) gAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGATTCTg < 1:2801892/71‑1 (MQ=255) atatAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTc < 1:2283453/49‑1 (MQ=255) atatAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTc < 1:1859439/49‑1 (MQ=255) tataGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGATTCTGGCaa < 1:2790223/71‑1 (MQ=255) tataGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGATTCTGGCa > 1:500098/1‑70 (MQ=255) ataGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGATTCTGGCaaa > 1:2911039/1‑71 (MQ=255) | GTTCTCGTGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCACTGCGTGATTCTGGCAAA > Exported/1416986‑1417124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |