| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 214,925 | A→C | 23.3% | D12A (GAT→GCT) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 214,925 | 0 | A | C | 23.3% | 141.0 / 76.8 | 103 | D12A (GAT→GCT) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base A (41/38); new base C (20/4); total (61/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.31e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGC > Exported/214855‑214994 | aaCTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGa < 1:2004147/71‑1 (MQ=255) tAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATAcc < 1:938783/70‑1 (MQ=255) tAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCg < 1:1699492/71‑1 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaa < 1:3179198/71‑1 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa > 1:1965490/1‑70 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa > 1:1452248/1‑70 (MQ=255) aaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaaa < 1:247816/71‑1 (MQ=255) aCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCTTTCTCGATACCGAAaccac < 1:3053629/71‑1 (MQ=255) aCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAaccac < 1:2347314/71‑1 (MQ=255) aCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAaccac < 1:2356384/71‑1 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGAt < 1:1703884/58‑1 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGAt < 1:3265758/58‑1 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTCCAATTACACGCCAGATCGTTCTCGAt < 1:1918436/58‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:1492087/70‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAACGCAACCACCGGTATg < 1:2265579/69‑1 (MQ=255) cATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaaa > 1:3178782/1‑57 (MQ=255) cATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaaa > 1:1316785/1‑57 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:3643067/1‑68 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAg < 1:2304466/70‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAATCCACCGGTATGAACCAGAt < 1:1893411/71‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc < 1:1566453/66‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc < 1:1657997/66‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc < 1:3603300/66‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc < 1:2602108/66‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAt < 1:2741474/71‑1 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt > 1:3219612/1‑71 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt > 1:1053498/1‑71 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt > 1:2765639/1‑71 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt > 1:2986291/1‑71 (MQ=255) gACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg > 1:669023/1‑71 (MQ=255) gACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTg > 1:242984/1‑71 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAcc < 1:1912206/64‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAcc < 1:1520379/64‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTgg < 1:752512/71‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTg < 1:387167/70‑1 (MQ=255) ccGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGt < 1:748434/71‑1 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTg > 1:2771606/1‑71 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTg > 1:3552569/1‑71 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTg > 1:3037875/1‑71 (MQ=255) gCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTgc > 1:1008439/1‑71 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg > 1:1818930/1‑71 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg > 1:763039/1‑71 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:756799/1‑46 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa > 1:3646227/1‑71 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa > 1:1803512/1‑71 (MQ=255) tGAGCACTGCAGTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc > 1:1932010/1‑71 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc > 1:1336669/1‑71 (MQ=255) gAGCACTGCAATTACTCGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc < 1:557781/55‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:1608107/71‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGATACCGaa < 1:3378038/36‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa > 1:78671/1‑65 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa > 1:1773687/1‑65 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa > 1:2878612/1‑65 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:529815/1‑69 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:1322714/1‑69 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa < 1:1033219/71‑1 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:2561682/1‑71 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:34432/1‑71 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:198503/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa > 1:683337/1‑61 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa > 1:589599/1‑61 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:895833/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg < 1:1315122/70‑1 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:2937592/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:3081658/1‑71 (MQ=255) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa < 1:1282010/49‑1 (MQ=255) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa < 1:2083339/49‑1 (MQ=255) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCGATATGAACCAGa < 1:3312644/49‑1 (MQ=255) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa < 1:2823579/48‑1 (MQ=255) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca > 1:2303042/1‑70 (MQ=255) tACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:221355/1‑70 (MQ=255) tcacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:473888/2‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa > 1:716795/1‑62 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa > 1:669825/1‑62 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa > 1:2896770/1‑62 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:3681138/1‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:1181712/1‑69 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa > 1:249080/1‑46 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt > 1:1089970/1‑48 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt > 1:1264266/1‑48 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAt > 1:2818475/1‑47 (MQ=255) cacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTgcgc > 1:912607/1‑54 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:56872/1‑60 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:1626344/1‑60 (MQ=255) ccAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa < 1:2543530/71‑1 (MQ=255) cAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa > 1:1145781/1‑57 (MQ=255) cAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa > 1:361240/1‑57 (MQ=255) cAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt > 1:526735/1‑71 (MQ=255) cAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt < 1:752982/71‑1 (MQ=255) cAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt > 1:2161866/1‑71 (MQ=255) gATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa < 1:3181540/38‑1 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:2911838/51‑1 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:2767360/51‑1 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:2606070/51‑1 (MQ=255) gTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGa > 1:1238653/1‑50 (MQ=255) ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgag < 1:1573132/68‑1 (MQ=255) tctcGATACCGAAACCACCGGTATGAACCAGATTGGTgcg > 1:3036124/1‑40 (MQ=255) gATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg < 1:184357/68‑1 (MQ=255) gATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGc > 1:1006451/1‑71 (MQ=255) aTACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg < 1:1394921/67‑1 (MQ=255) | AACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGC > Exported/214855‑214994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |