| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,558,459 | C→G | 11.1% | L167F (TTG→TTC) | emrK ← | EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,558,459 | 0 | C | G | 11.1% | 175.5 / 11.9 | 63 | L167F (TTG→TTC) | emrK | EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component |
| Reads supporting (aligned to +/- strand): ref base C (31/25); new base G (2/5); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCC > Exported/1558390‑1558524 | tgGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTc < 1:1576657/70‑1 (MQ=255) gCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCaa < 1:2557678/70‑1 (MQ=255) tGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGc > 1:1696945/1‑71 (MQ=255) gACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGcc > 1:2298832/1‑71 (MQ=255) aCGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCg > 1:2184358/1‑71 (MQ=255) ggTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTGAATGCCGCt < 1:3484873/71‑1 (MQ=255) ggTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTGAATGCCGCt < 1:1693657/71‑1 (MQ=255) ggTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCaa < 1:410078/64‑1 (MQ=255) ggTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCt < 1:3067635/71‑1 (MQ=255) gTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCtt < 1:2272922/71‑1 (MQ=255) gTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCtt < 1:2125725/71‑1 (MQ=255) tttAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTGAATGCCGCt < 1:2372768/69‑1 (MQ=255) tttAATGGCGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCttt < 1:3105348/71‑1 (MQ=255) aaTGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTc < 1:2587212/57‑1 (MQ=255) aaTGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTg > 1:1806221/1‑69 (MQ=255) aaTGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGc > 1:3426908/1‑70 (MQ=255) aaTGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGc < 1:855210/70‑1 (MQ=255) aTGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCt < 1:2555295/70‑1 (MQ=255) aTGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTa > 1:3957/1‑71 (MQ=255) tGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTAc < 1:746524/71‑1 (MQ=255) gtTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACttatt > 1:3193629/1‑70 (MQ=255) gtTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACttatt > 1:2128047/1‑70 (MQ=255) ttCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACttatta < 1:981952/70‑1 (MQ=255) ttCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACttatta < 1:868970/70‑1 (MQ=255) aTTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCg < 1:183500/52‑1 (MQ=255) aTTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCg < 1:704629/52‑1 (MQ=255) ttACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCa > 1:852964/1‑45 (MQ=255) ttACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCa > 1:2298234/1‑45 (MQ=255) ttACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTa < 1:847411/71‑1 (MQ=255) ttACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTa < 1:3221911/71‑1 (MQ=255) aCTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATc > 1:911705/1‑71 (MQ=255) tAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCtt > 1:2542462/1‑71 (MQ=255) gCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCaa > 1:821412/1‑38 (MQ=255) gCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTgg > 1:1953539/1‑70 (MQ=255) tttATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCtt < 1:3539623/65‑1 (MQ=255) ttATTCGCTTTATAAGCCTGGATAGCGGCATTCaa < 1:2053904/35‑1 (MQ=255) ttATTCGCTTTATAAGCCTGGATAGCGGCATTCaa < 1:1075979/35‑1 (MQ=255) tATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGc > 1:2479374/1‑71 (MQ=255) aTTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTa > 1:333308/1‑67 (MQ=255) ttCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACttatt < 1:190179/51‑1 (MQ=255) gCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCa < 1:460049/70‑1 (MQ=255) gCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAg > 1:789015/1‑71 (MQ=255) ttATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTAt > 1:2126084/1‑52 (MQ=255) tataAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGtt > 1:1125227/1‑71 (MQ=255) aaGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTcc > 1:1621259/1‑62 (MQ=255) ccTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTa > 1:1883668/1‑53 (MQ=255) cTGGATAGCGGCATTCAATGCCGCTTTGCTACttatt > 1:1164856/1‑37 (MQ=255) cTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCtttt > 1:2502317/1‑70 (MQ=255) cTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCtttt < 1:2382057/70‑1 (MQ=255) ggATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGa > 1:519582/1‑70 (MQ=255) gATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGaaa > 1:2373961/1‑71 (MQ=255) gATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGaa < 1:1227254/70‑1 (MQ=255) gATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGaa < 1:404497/70‑1 (MQ=255) gATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGaa < 1:848674/70‑1 (MQ=255) aTAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGaaa > 1:1520340/1‑70 (MQ=255) aTAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGaaa > 1:2254248/1‑70 (MQ=255) aTAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGaa > 1:3396480/1‑69 (MQ=255) tAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATa < 1:3604188/71‑1 (MQ=255) aaGCGGCATTGAATGCCGCTTTGCTACTTATTAACGTATc < 1:819442/39‑1 (MQ=37) aaGCGGCATTGAATGCCGCTTTGCTACTTATTAACGTATc < 1:894284/39‑1 (MQ=37) aGCGGCATTGAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATaa > 1:503942/1‑71 (MQ=255) gCGGCATTGAATGCCGCTTTGCTACTTATTAACGTATCtt > 1:3423886/1‑40 (MQ=255) gCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAAcccc > 1:2523100/1‑71 (MQ=255) gCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAAcc > 1:723309/1‑69 (MQ=255) gCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAAcc > 1:85075/1‑69 (MQ=255) cAATGCCGCTTTGCTACTTATTAACGTATCTTTgg > 1:2795683/1‑35 (MQ=255) cAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTa > 1:767130/1‑37 (MQ=255) cAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTa > 1:472153/1‑37 (MQ=255) cAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTa > 1:1300975/1‑37 (MQ=255) cAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTc < 1:1821272/42‑1 (MQ=255) | TGGCTGACGGTTTAATGGTGTGTTCATTACTAAAGCTTTATTCGCTTTATAAGCCTGGATAGCGGCATTCAATGCCGCTTTGCTACTTATTAACGTATCTTTGGTATGCTCCAGCGTTTCTTTTGAAATAACCCC > Exported/1558390‑1558524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |