Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,559,532 T→A 8.3% V53D (GTC→GAC)  evgA → DNA‑binding response regulator in two‑component regulatory system with EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,559,5320TA 8.3% 244.3 / 11.7 84V53D (GTC→GAC) evgADNA‑binding response regulator in two‑component regulatory system with EvgS
Reads supporting (aligned to +/- strand):  ref base T (41/36);  new base A (2/5);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

AGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGG  >  Exported/1559463‑1559595
                                                                     |                                                               
agTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                <  1:1512434/71‑1 (MQ=255)
 gTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                <  1:1330525/70‑1 (MQ=255)
      cTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATc                                                          >  1:1277043/1‑71 (MQ=255)
         aaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                 >  1:1710515/1‑61 (MQ=255)
         aaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                 >  1:2824008/1‑61 (MQ=255)
         aaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATcccc                                                       >  1:680331/1‑71 (MQ=255)
         aaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATcccc                                                       >  1:1950004/1‑71 (MQ=255)
                aaGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                   >  1:1729418/1‑68 (MQ=255)
                aaGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGtt                                                 >  1:3042092/1‑70 (MQ=255)
                aaGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGtt                                                 >  1:881850/1‑70 (MQ=255)
                aaGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTa                                                <  1:2347087/71‑1 (MQ=255)
                 aGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTaa                                               <  1:3591593/71‑1 (MQ=255)
                  gTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                   >  1:702097/1‑66 (MQ=255)
                  gTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                   >  1:163325/1‑66 (MQ=255)
                    gCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACg                                                               <  1:708619/52‑1 (MQ=25)
                    gCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACg                                                               <  1:170705/52‑1 (MQ=25)
                     ccGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACgg                                            >  1:1041459/1‑70 (MQ=255)
                     ccGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACgg                                            >  1:371937/1‑70 (MQ=255)
                       gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                   >  1:3715945/1‑61 (MQ=255)
                       gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                   >  1:2235097/1‑61 (MQ=255)
                       gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                   >  1:1252556/1‑61 (MQ=255)
                       gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTAt                                         >  1:1606494/1‑71 (MQ=255)
                       gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTAt                                         >  1:2564365/1‑71 (MQ=255)
                       gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTAt                                         >  1:961732/1‑71 (MQ=255)
                       gTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTAt                                         >  1:2979531/1‑71 (MQ=255)
                        ttCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATc                                        >  1:2892192/1‑71 (MQ=255)
                         tCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATc                                        >  1:844206/1‑70 (MQ=255)
                          cAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATcc                                       <  1:1704301/70‑1 (MQ=255)
                          cAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATc                                        >  1:1320312/1‑69 (MQ=255)
                           aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCa                                      <  1:1554278/70‑1 (MQ=255)
                              gggTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                  >  1:667712/1‑71 (MQ=255)
                               ggTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtgt                                 <  1:162293/71‑1 (MQ=255)
                                   gAAACACTTAAGCCTGATATCTTCATCATTGATGTCGATATCCCCGGAGTTATCGGTATCCAg                                     <  1:3552389/63‑1 (MQ=255)
                                   gAAACACTTAAGCCTGATATCGTCATCATTGATGTCg                                                               <  1:2392396/37‑1 (MQ=255)
                                   gAAACACTTAAGCCTGATATCGTCATCATTGATGTCg                                                               <  1:2214908/37‑1 (MQ=255)
                                   gAAACACTTAAGCCTGATATCGTCATCATTGATGTCg                                                               <  1:3700327/37‑1 (MQ=255)
                                   gAAACACTTAAGCCTGATATCGTCATCATTGATGTCg                                                               <  1:1514140/37‑1 (MQ=255)
                                   gAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAg                              >  1:1080782/1‑70 (MQ=255)
                                   gAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGa                             <  1:127256/71‑1 (MQ=255)
                                    aaaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGa                             >  1:3616007/1‑70 (MQ=255)
                                    aaaCACTTAAGCCTGATATCGACATCAATGATGACGa                                                              <  1:1977136/37‑1 (MQ=17)
                                    aaaCACTTAAGCCTGATATCGACATCAATGATGACGa                                                              <  1:1840004/37‑1 (MQ=17)
                                    aaaCACTTAAGCCTGATATCGACATCAATGATGACGa                                                              <  1:2282818/37‑1 (MQ=17)
                                     aaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAAc                                              >  1:100926/1‑52 (MQ=255)
                                     aaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgt                                   >  1:1427289/1‑63 (MQ=255)
                                     aaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgt                                   >  1:2654504/1‑63 (MQ=255)
                                     aaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaaa                           <  1:3086605/71‑1 (MQ=255)
                                      acacTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGaaa                           >  1:1518301/1‑70 (MQ=255)
                                      acacTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAAc                          >  1:164638/1‑71 (MQ=255)
                                      acacTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAAc                          >  1:3008599/1‑71 (MQ=255)
                                      acacTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAAc                          >  1:3026372/1‑71 (MQ=255)
                                      acacTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAAc                          >  1:3031913/1‑71 (MQ=255)
                                      acacTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATACAGGTGTTAGaaa                           >  1:664502/1‑70 (MQ=255)
                                        acTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGc                        >  1:2565320/1‑71 (MQ=255)
                                         cTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCt                       <  1:3559450/71‑1 (MQ=255)
                                         cTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCt                       <  1:2272034/71‑1 (MQ=255)
                                          ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAg                              <  1:2967667/63‑1 (MQ=255)
                                           tAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                      >  1:2840873/1‑38 (MQ=255)
                                           tAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                      >  1:2836595/1‑38 (MQ=255)
                                            aaGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAAc                                              <  1:679824/45‑1 (MQ=255)
                                            aaGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAAc                                              <  1:3309463/45‑1 (MQ=255)
                                            aaGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAAc                                              <  1:3359962/45‑1 (MQ=255)
                                             aGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAgg                   <  1:985052/71‑1 (MQ=255)
                                             aGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAg                    >  1:764164/1‑70 (MQ=255)
                                                 tGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGaa                 <  1:811720/69‑1 (MQ=255)
                                                 tGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgc               >  1:2047059/1‑71 (MQ=255)
                                                 tGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgc               >  1:725405/1‑71 (MQ=255)
                                                 tGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgc               >  1:2402595/1‑71 (MQ=255)
                                                 tGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAg                >  1:2574669/1‑70 (MQ=255)
                                                  gATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCt                       <  1:2223734/62‑1 (MQ=255)
                                                      tCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCaa          <  1:2690771/71‑1 (MQ=255)
                                                       cgacatcaaTGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCaa          >  1:3282596/4‑70 (MQ=38)
                                                        gacatcaaTGATGACGATATCCCCGGAGTTAACGGTATCCAGgt                                   >  1:2778436/3‑44 (MQ=25)
                                                        gTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAat         >  1:2251080/1‑70 (MQ=255)
                                                         tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtgt                                 <  1:3573938/45‑1 (MQ=255)
                                                         tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtgt                                 <  1:2947640/45‑1 (MQ=255)
                                                         tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcg              <  1:2521156/64‑1 (MQ=255)
                                                         tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcg              <  1:3662683/64‑1 (MQ=255)
                                                         tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcg              <  1:1396283/64‑1 (MQ=255)
                                                         tcatcaTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAatat       <  1:2924574/71‑1 (MQ=255)
                                                           atcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAAtata      <  1:1364619/70‑1 (MQ=255)
                                                            tcatTGATGTCGATATCCCCGGAGTTAACGGTATc                                        <  1:419422/35‑1 (MQ=255)
                                                            tcatTGATGTCGATATCCCCGGAGTTAACGGTATc                                        <  1:714158/35‑1 (MQ=255)
                                                              atTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCgg  <  1:3391971/71‑1 (MQ=255)
                                                              atTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCg   <  1:2633890/70‑1 (MQ=255)
                                                                   tGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAata        <  1:3003953/60‑1 (MQ=255)
                                                                     |                                                               
AGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGG  >  Exported/1559463‑1559595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: