Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,614,492 T→C 13.1% intergenic (‑117/+100) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,614,4920TC13.1% 100.6 / 7.5 38intergenic (‑117/+100)eutH/eutGpredicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base T (20/13);  new base C (3/2);  total (23/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

AACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTG  >  Exported/1614422‑1614560
                                                                      |                                                                    
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt                                                                      >  1:1102033/1‑71 (MQ=38)
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt                                                                      >  1:2314229/1‑71 (MQ=38)
      ttATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACACGCGTCGCATCCGGCATCTGCAcc                                                                >  1:2941310/1‑71 (MQ=2)
       tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcg                                                               >  1:2568436/1‑71 (MQ=255)
       tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcg                                                               >  1:2218967/1‑71 (MQ=255)
        aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAcc                                                                <  1:729170/69‑1 (MQ=255)
        aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAcc                                                                <  1:3357276/69‑1 (MQ=255)
        aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc                                                              >  1:2090673/1‑71 (MQ=255)
                         aaTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcgg                                             >  1:3576995/1‑71 (MQ=255)
                          aTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggc                                            >  1:1471962/1‑71 (MQ=255)
                          aTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggc                                            >  1:2761249/1‑71 (MQ=255)
                             ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGt                                          <  1:124834/70‑1 (MQ=255)
                               gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc                                                     <  1:995829/57‑1 (MQ=255)
                               gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc                                                     <  1:2260157/57‑1 (MQ=255)
                               gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc                                                     <  1:1570692/57‑1 (MQ=255)
                               gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGaa                                       >  1:14397/1‑71 (MQ=255)
                               gTAGGCTGGATAAGACGCGGCAAGAGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGaa                                       >  1:2664176/1‑71 (MQ=255)
                                tAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc                                      >  1:3574566/1‑71 (MQ=255)
                                  ggCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACg                                     <  1:3117542/70‑1 (MQ=255)
                                  ggCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACg                                     <  1:198407/70‑1 (MQ=255)
                                     cggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCtt                                 <  1:2218929/70‑1 (MQ=17)
                                       gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCtt                                 <  1:283805/69‑1 (MQ=255)
                                        aTAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc                              >  1:1888818/1‑71 (MQ=255)
                                         tAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATcc                             <  1:156536/71‑1 (MQ=255)
                                              cgcgGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc                         >  1:517813/1‑70 (MQ=255)
                                               gcgACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTa                       >  1:1915980/1‑71 (MQ=18)
                                                   cAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtg                   <  1:374967/71‑1 (MQ=255)
                                                    aaGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgt                  >  1:993088/1‑71 (MQ=255)
                                                    aaGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgt                  >  1:314177/1‑71 (MQ=255)
                                                        gTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCaa              >  1:1553563/1‑71 (MQ=255)
                                                           gCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATcc           >  1:2602075/1‑71 (MQ=255)
                                                           gCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCCCCCTACATGTGCAATcc           <  1:2664854/71‑1 (MQ=255)
                                                             aTCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCg         <  1:2355952/71‑1 (MQ=255)
                                                                 ggCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCaa              >  1:2919257/1‑62 (MQ=255)
                                                                 ggCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg     <  1:3194126/71‑1 (MQ=255)
                                                                 ggCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAg      >  1:2225496/1‑70 (MQ=255)
                                                                  gCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc    >  1:3698537/1‑71 (MQ=255)
                                                                   cAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtg                   >  1:2392681/1‑55 (MQ=255)
                                                                   cAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtg                   >  1:2949101/1‑55 (MQ=255)
                                                                     aTTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTAACCGCCCTACATGTGCAATCCCGTAGGCTg  >  1:2017781/1‑70 (MQ=255)
                                                                      |                                                                    
AACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTG  >  Exported/1614422‑1614560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: