| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,618,811 | C→T | 13.9% | V321V (GTG→GTA) | eutI ← | predicted phosphotransacetylase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,618,811 | 0 | C | T | 13.9% | 92.9 / 7.3 | 36 | V321V (GTG→GTA) | eutI | predicted phosphotransacetylase subunit |
| Reads supporting (aligned to +/- strand): ref base C (23/8); new base T (2/3); total (25/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
TCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCA > Exported/1618741‑1618875 | tCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGc < 1:99862/71‑1 (MQ=255)tCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGc < 1:96452/71‑1 (MQ=255) aGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGcaca < 1:2794596/71‑1 (MQ=255) gAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAg > 1:3540928/1‑71 (MQ=255) gAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAg > 1:3341895/1‑71 (MQ=255) gAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGATTCACTTCTGTCTGACGCGGCACAg > 1:1745285/1‑71 (MQ=255) aCGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCt < 1:1277090/71‑1 (MQ=255) aCGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCt > 1:1410149/1‑71 (MQ=255) ggACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGc > 1:1542663/1‑70 (MQ=255) ggACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGc > 1:481677/1‑70 (MQ=255) gACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGcca > 1:2381895/1‑71 (MQ=255) ccGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGccac < 1:422230/70‑1 (MQ=255) tcattcaACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAgcg > 1:131795/1‑71 (MQ=255) tcattcaACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAgcg > 1:658352/1‑71 (MQ=255) cattcaACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAgcgc > 1:3598908/1‑71 (MQ=255) cattcaACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAgcgc > 1:2411693/1‑71 (MQ=255) caACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAg < 1:1779496/70‑1 (MQ=255) aGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGAt < 1:1239350/71‑1 (MQ=255) gtgtTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGa > 1:1095866/1‑69 (MQ=255) gtgtTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGAt > 1:2241581/1‑70 (MQ=255) tttGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAgcg < 1:1596122/57‑1 (MQ=255) tGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGTACAg > 1:2579409/1‑43 (MQ=255) tGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGTACAGCTGCCACCAGCGCCAGCTCGATAAttt < 1:827263/70‑1 (MQ=255) tAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTcc < 1:569244/70‑1 (MQ=255) aaCTGCTTTCGCGGTTCACTTCTGTCTGACGCGGTACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTg < 1:1273392/70‑1 (MQ=255) tGCTTTCGCGGTTCACTTCTGTCTGACGCGGTACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACg > 1:1244791/1‑71 (MQ=255) cTTTCGCGGTTCACTTCTGTCTGACGCGGTACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACg < 1:3628869/69‑1 (MQ=255) cTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGc > 1:3651093/1‑70 (MQ=255) cTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGc < 1:2449734/70‑1 (MQ=255) tCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTAc > 1:1715798/1‑70 (MQ=255) tCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTAc > 1:3012235/1‑70 (MQ=255) gcgGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTa > 1:1606364/1‑67 (MQ=255) cTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCa > 1:668304/1‑54 (MQ=255) cTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCa > 1:3228485/1‑54 (MQ=255) ctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgaga > 1:1663737/1‑67 (MQ=255) ctgtctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgaga > 1:743374/1‑67 (MQ=255) tctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCg > 1:69598/1‑71 (MQ=255) ctgACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCt > 1:3242996/1‑44 (MQ=255) aCGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCa < 1:533936/71‑1 (MQ=255) | TCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCA > Exported/1618741‑1618875 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |