Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,635,547 T→C 6.8% F562S (TTC→TCC)  acrD → aminoglycoside/multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,635,5470TC 6.8% 208.1 / 5.9 73F562S (TTC→TCC) acrDaminoglycoside/multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base T (28/40);  new base C (2/3);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

CGCTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGCAACAACA  >  Exported/1635477‑1635616
                                                                      |                                                                     
cgcTGGATTGTGATTTATGTCCTGATGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGtt                                                                       >  1:3239769/1‑71 (MQ=255)
  cTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCt                                                                     <  1:2101764/71‑1 (MQ=255)
  cTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCt                                                                     <  1:2322136/71‑1 (MQ=255)
    ggATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTa                                                                   >  1:2514400/1‑71 (MQ=255)
     gATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCtt                                                                    <  1:2842051/69‑1 (MQ=255)
     gATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTAc                                                                  >  1:2212615/1‑71 (MQ=255)
       ttGTGATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCg                                                                >  1:389044/1‑71 (MQ=255)
        tgtgATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGc                                                               >  1:794624/1‑71 (MQ=255)
          tgATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCt                                                              <  1:3618919/70‑1 (MQ=255)
          tgATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCt                                                              <  1:1256389/70‑1 (MQ=255)
            aTTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTgg                                                            >  1:2101191/1‑70 (MQ=255)
            aTTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTgg                                                            <  1:2305190/70‑1 (MQ=255)
            aTTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTCCTTACCGCTgg                                                            <  1:495607/70‑1 (MQ=255)
            aTTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTCCTTACCGCTgg                                                            >  1:145581/1‑70 (MQ=255)
              ttATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTgg                                                            >  1:1060525/1‑68 (MQ=255)
               tATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAg                                                         <  1:2088845/70‑1 (MQ=255)
               tATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAg                                                         <  1:3045598/70‑1 (MQ=255)
                 tGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGAcc                                                      <  1:2304648/71‑1 (MQ=255)
                    ccTGCTGCTTGGCGGCATGGTTTTCCTGTTCCTGCGTTTGCCGACGTCGTTca                                                                     <  1:2815572/53‑2 (MQ=255)
                    ccTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTg                                                   >  1:921298/1‑71 (MQ=255)
                     ctgctgCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCt                                                                     <  1:3203881/52‑1 (MQ=255)
                     ctgctgCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCt                                                                     <  1:1237009/52‑1 (MQ=255)
                     ctgctgCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTg                                                   <  1:1424105/70‑1 (MQ=255)
                       gctgctTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCa                                                >  1:897199/1‑71 (MQ=255)
                       gctgctTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCa                                                >  1:486347/1‑71 (MQ=255)
                       gctgctTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCa                                                >  1:1481770/1‑71 (MQ=255)
                        ctgctTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCa                                                >  1:71200/1‑70 (MQ=255)
                           ctTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGt                                             >  1:1001307/1‑70 (MQ=255)
                           ctTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGt                                             <  1:2271869/70‑1 (MQ=255)
                             tGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGt                                             >  1:758452/1‑68 (MQ=255)
                             tGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGt                                             >  1:3234597/1‑68 (MQ=255)
                             tGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGt                                             >  1:223865/1‑68 (MQ=255)
                             tGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGt                                             >  1:1427913/1‑68 (MQ=255)
                             tGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTta                                          >  1:2461331/1‑71 (MQ=255)
                              ggcggcATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTCCTTACCGCTGGAAGACCGTTGCATGTTta                                          <  1:1775719/70‑1 (MQ=255)
                                  gcATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGaa                                                          >  1:1104380/1‑50 (MQ=255)
                                     tGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCg                                  >  1:1513063/1‑71 (MQ=255)
                                      ggTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCa                                                <  1:2722807/56‑1 (MQ=255)
                                      ggTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGAATg                                              <  1:1341595/58‑1 (MQ=255)
                                           tcctgttcctgCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAg                            >  1:2713002/1‑71 (MQ=255)
                                            cctgttcctgCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAg                            <  1:2626598/70‑1 (MQ=255)
                                            cctgttcctgCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAg                            <  1:2926580/70‑1 (MQ=255)
                                            cctgttcctgCGTTTGCCGACGTCGTCCTTACCGCTGGAAGACCGTGGCATATTTACTACCTCGGTACAGt                           >  1:2569791/1‑71 (MQ=255)
                                              tgttcctgCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATg                                              <  1:1514891/50‑1 (MQ=255)
                                                   ctgCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAg                    <  1:241978/71‑1 (MQ=255)
                                                   ctgCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAg                    <  1:536975/71‑1 (MQ=255)
                                                   ctgCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAg                    <  1:1813276/71‑1 (MQ=255)
                                                      cGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTtact                                        >  1:497920/1‑48 (MQ=255)
                                                      cGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACATTTGCCCAGCgg                 <  1:108751/71‑1 (MQ=255)
                                                      cGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGc                   <  1:1581787/69‑1 (MQ=255)
                                                      cGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCgg                 <  1:2373970/71‑1 (MQ=255)
                                                      cGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCg                  <  1:1787880/70‑1 (MQ=255)
                                                        tttGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGtt               <  1:2431557/71‑1 (MQ=255)
                                                          tGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTc              >  1:3698473/1‑70 (MQ=255)
                                                           gCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCa             <  1:1816286/70‑1 (MQ=255)
                                                           gCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCa             <  1:2751570/70‑1 (MQ=255)
                                                           gCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCa             <  1:1307859/70‑1 (MQ=255)
                                                           gCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCa             <  1:3341099/70‑1 (MQ=255)
                                                           gCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCa             <  1:1757428/70‑1 (MQ=255)
                                                           gCCGACGTCGATCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCa             <  1:2621894/70‑1 (MQ=255)
                                                            ccGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAAc           <  1:1977627/71‑1 (MQ=255)
                                                              gACGTCGTCCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGc         <  1:2238379/71‑1 (MQ=255)
                                                               aCGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAg                            <  1:2545690/51‑1 (MQ=255)
                                                               aCGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACg          >  1:163082/1‑69 (MQ=255)
                                                               aCGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGca        <  1:1384491/71‑1 (MQ=255)
                                                               aCGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGca        <  1:2442908/71‑1 (MQ=255)
                                                               aCGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGca        <  1:2684413/71‑1 (MQ=255)
                                                               aCGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGca        <  1:1333458/71‑1 (MQ=255)
                                                               aCGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGc         >  1:860816/1‑70 (MQ=255)
                                                               aCGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGc         >  1:1150027/1‑70 (MQ=255)
                                                               aCGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGc         >  1:2765316/1‑70 (MQ=255)
                                                                 gtcgtTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTg                         >  1:277997/1‑52 (MQ=255)
                                                                 gtcgtTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTc              >  1:3199329/1‑63 (MQ=255)
                                                                 gtcgtTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGcaac      <  1:3313826/71‑1 (MQ=255)
                                                                     ttCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGCAacaaca  <  1:726621/71‑1 (MQ=255)
                                                                      tCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACg          >  1:2286377/1‑62 (MQ=255)
                                                                      tCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACTCAacaaca  >  1:3382900/1‑70 (MQ=255)
                                                                      tCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGcaacaa    >  1:3692980/1‑68 (MQ=255)
                                                                      tCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGcaacaa    >  1:3688790/1‑68 (MQ=255)
                                                                      |                                                                     
CGCTGGATTGTGATTTATGTCCTGCTGCTTGGCGGCATGGTGTTCCTGTTCCTGCGTTTGCCGACGTCGTTCTTACCGCTGGAAGACCGTGGCATGTTTACTACCTCGGTACAGTTGCCCAGCGGTTCAACGCAACAACA  >  Exported/1635477‑1635616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: