| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,738,791 | G→A | 59.6% | C340C (TGC→TGT) | lepA ← | GTP binding membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,738,791 | 0 | G | A | 59.6% | 25.2 / 54.6 | 47 | C340C (TGC→TGT) | lepA | GTP binding membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (4/15); new base A (5/23); total (9/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTC > Exported/1738722‑1738854 | cagatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCg < 1:1386624/70‑1 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAg < 1:987585/71‑1 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAg < 1:2879695/71‑1 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAg < 1:3100228/71‑1 (MQ=255) atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGc > 1:751354/1‑71 (MQ=255) atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGc > 1:254531/1‑71 (MQ=255) cagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGa < 1:3172500/71‑1 (MQ=255) agatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGaa < 1:2164931/71‑1 (MQ=255) gatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGaaa < 1:698903/71‑1 (MQ=255) atcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAAc < 1:3167543/71‑1 (MQ=255) tcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAAcc < 1:3031611/71‑1 (MQ=255) gTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCaa < 1:1033688/71‑1 (MQ=255) aCGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGccc < 1:3184090/70‑1 (MQ=255) gTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAgc < 1:1777260/71‑1 (MQ=255) ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCa < 1:724521/61‑1 (MQ=255) ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCa < 1:696135/61‑1 (MQ=255) ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCa < 1:3262663/61‑1 (MQ=255) ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCa < 1:1431088/61‑1 (MQ=255) ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCa < 1:859341/61‑1 (MQ=255) cAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAgcgcgc < 1:1589788/71‑1 (MQ=255) aGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAgcgcg < 1:2080742/69‑1 (MQ=255) aGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCt < 1:2999730/71‑1 (MQ=255) aCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCTGCAGCGGAAACCAAAGCCCAGCGCGCTg > 1:154783/1‑70 (MQ=255) gTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGTAAACCAAAGcc < 1:2902098/58‑1 (MQ=255) gTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGcc < 1:2781404/58‑1 (MQ=255) gTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAg > 1:3173840/1‑71 (MQ=255) cTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTGGAGCttt < 1:3308757/71‑1 (MQ=255) cTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTGGAGCtt < 1:3022965/70‑1 (MQ=255) gatgatCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTcc < 1:2827335/70‑1 (MQ=255) gatgatCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTc < 1:3031562/69‑1 (MQ=255) gatgatCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg < 1:7762/71‑1 (MQ=255) gatgatCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg > 1:2228454/1‑71 (MQ=255) atgatCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCgg < 1:2859492/71‑1 (MQ=255) atCTCCATGTGCAGCATGCCGAGGAAGCCACAGCGGAAAcc < 1:654564/41‑1 (MQ=37) atCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAAcc < 1:2781730/41‑1 (MQ=255) atCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAAcc < 1:3513699/41‑1 (MQ=255) ccATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAg > 1:1390548/1‑46 (MQ=255) cATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGc < 1:3561832/64‑1 (MQ=255) aTGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGaa < 1:1803710/71‑1 (MQ=255) tgtgCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGaa > 1:1448838/1‑70 (MQ=255) tgCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa < 1:2582610/70‑1 (MQ=255) tgCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCAAGCGCGCTGGAGCTTTCCGGCTCATAGAACa < 1:2953186/70‑1 (MQ=255) cagcagGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTg < 1:1991650/71‑1 (MQ=255) ggCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCa < 1:2069787/71‑1 (MQ=255) ggCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCa < 1:3151291/71‑1 (MQ=255) cGAGGCAGCCACAGCGGAACCCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc > 1:1910420/1‑67 (MQ=255) ggAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTc < 1:2331631/71‑1 (MQ=255) aaGCCACAGCGGAAACCAAAGCCCAGCGCGCTGGa > 1:1378041/1‑35 (MQ=255) | CAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTC > Exported/1738722‑1738854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |