Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 1,739,943 | T→C | 46.4% | intergenic (‑133/+65) | lepA ← / ← rseC | GTP binding membrane protein/RseC protein involved in reduction of the SoxR iron‑sulfur cluster |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 1,739,943 | 0 | T | C | 46.4% | 8.9 / 97.1 | 69 | intergenic (‑133/+65) | lepA/rseC | GTP binding membrane protein/RseC protein involved in reduction of the SoxR iron‑sulfur cluster |
Reads supporting (aligned to +/- strand): ref base T (20/17); new base C (19/13); total (39/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.08e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 |
ACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCA > Exported/1739877‑1740010 | aCGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATg > 1:2456299/1‑70 (MQ=255) gTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGAt > 1:2043834/1‑71 (MQ=255) gTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGAt > 1:2506525/1‑71 (MQ=255) cTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATtg > 1:682010/1‑71 (MQ=255) tATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATtg < 1:2166457/68‑1 (MQ=255) tATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATtg < 1:2650341/68‑1 (MQ=255) aaTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATc > 1:1347626/1‑71 (MQ=255) aTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTAt < 1:2355933/69‑1 (MQ=255) tAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTg > 1:2271136/1‑71 (MQ=255) tAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTg > 1:1214748/1‑71 (MQ=255) tAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTg > 1:2620524/1‑71 (MQ=255) gAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATtgtg > 1:1700992/1‑62 (MQ=255) aaaCGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGaa < 1:2417980/71‑1 (MQ=255) aaCGCCGCATTTTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGaaa < 1:2077811/71‑1 (MQ=255) cgcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGaaa < 1:159873/66‑1 (MQ=255) cATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTc > 1:1643407/1‑71 (MQ=255) cATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTc > 1:2246855/1‑71 (MQ=255) aTTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCg > 1:499903/1‑71 (MQ=255) ttCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCg > 1:843347/1‑70 (MQ=255) ctacactacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAt > 1:608872/1‑71 (MQ=255) tacactacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTg > 1:1729922/1‑55 (MQ=255) tacactacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAtt > 1:3699777/1‑71 (MQ=255) acactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGaa > 1:2032766/1‑57 (MQ=255) acactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGa > 1:473451/1‑56 (MQ=255) cactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCAttt < 1:2844408/70‑1 (MQ=255) actacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGt > 1:2019675/1‑71 (MQ=255) actacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGt > 1:179161/1‑71 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGa < 1:22111/50‑1 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGa < 1:1326974/50‑1 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGa < 1:126447/50‑1 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGa < 1:3635540/50‑1 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGa < 1:269499/50‑1 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGCATgg < 1:3606183/35‑1 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGtt < 1:603277/68‑1 (MQ=255) caacaTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGtt < 1:891198/68‑1 (MQ=255) caTTGAGGCGAGGAAATGTTCATACCGCATGTATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCaa < 1:742220/71‑1 (MQ=255) ttGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCaaaa < 1:1168039/71‑1 (MQ=255) ttGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCaaaa < 1:1023576/71‑1 (MQ=255) aGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGc < 1:3710701/71‑1 (MQ=255) aGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGc < 1:1482007/71‑1 (MQ=255) cGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCaaaa < 1:1147108/65‑1 (MQ=255) gAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGcaaca < 1:344042/71‑1 (MQ=255) ggAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGcaaca > 1:3299943/1‑69 (MQ=255) ggAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGcaaca > 1:3464841/1‑69 (MQ=255) aaTGTTCATACCGTATGGATTGTGGTATCTGGAAACg > 1:1199585/1‑37 (MQ=255) aaTGTTCATACCGTATGGATTGTGGTATCTGGAAACg > 1:2652066/1‑37 (MQ=255) aTGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACTAAGCCa < 1:5717/71‑1 (MQ=255) aTGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGGCa < 1:393112/71‑1 (MQ=255) aTGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCa < 1:3488925/71‑1 (MQ=255) aTGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCa < 1:3316232/71‑1 (MQ=255) ttCATACCGTATGGATTGTGGTATCTGGAAACGTCCt > 1:3459918/1‑37 (MQ=255) ttCATACCGTATGGATTGTGGTATCTGGAAACGTCCt > 1:751434/1‑37 (MQ=255) ttCATACCGTATGGATTGTGGTATCTGGAAACGTCCt > 1:2257632/1‑37 (MQ=255) ttCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGt < 1:344783/70‑1 (MQ=255) ttCATACCGCATGGATTGTGGTATCTGGAAACGTCCTc > 1:64051/1‑38 (MQ=255) ttCATACCGCATGGATTGTGGTATCTGGAAACGTCCTc > 1:2470072/1‑38 (MQ=255) ttCATACCGCATGGATTGTGGTATCTGGAAACGTCCTc > 1:1820967/1‑38 (MQ=255) ttCATACCGCATGGATTGTGGTATCTGGAAACGTCCTc > 1:2062744/1‑38 (MQ=255) ttCATACCGCATGGATTGTGGTATCTGGAAACGTCCTc > 1:2099467/1‑38 (MQ=255) tCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGcc > 1:3104464/1‑66 (MQ=255) tCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGcc > 1:3503075/1‑66 (MQ=255) cATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACaaa > 1:1566077/1‑62 (MQ=255) cATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACaaa > 1:1440833/1‑62 (MQ=255) cATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACaaa > 1:869006/1‑62 (MQ=255) aTACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGaa < 1:2146995/70‑1 (MQ=255) ccGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATc < 1:3442792/70‑1 (MQ=255) ccGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCTACAAAGCCAGTGAAATCa > 1:3415784/1‑71 (MQ=255) ccGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCa > 1:2134293/1‑71 (MQ=255) ccGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCa < 1:161776/71‑1 (MQ=255) | ACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCA > Exported/1739877‑1740010 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |