| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,744,552 | G→A | 65.9% | V483I (GTC→ATC) | nadB → | quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,744,552 | 0 | G | A | 65.9% | 46.4 / 38.4 | 44 | V483I (GTC→ATC) | nadB | quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
| Reads supporting (aligned to +/- strand): ref base G (7/8); new base A (21/8); total (28/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAAT > Exported/1744482‑1744619 | ccTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCcgcg > 1:1431314/1‑71 (MQ=255) tGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc > 1:3113030/1‑71 (MQ=255) ggAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtct > 1:2844447/1‑71 (MQ=255) ggAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtct > 1:384045/1‑71 (MQ=255) aaCGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGtctc > 1:2879757/1‑70 (MQ=255) aaCGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGtctc > 1:2102392/1‑70 (MQ=255) gcgcCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCa < 1:1037959/68‑1 (MQ=255) gcggcgGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGc > 1:1182794/1‑71 (MQ=255) gcggcgGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGc > 1:3671317/1‑71 (MQ=255) cggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTgg < 1:3255943/70‑1 (MQ=255) ggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg < 1:2610782/71‑1 (MQ=255) ggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg < 1:1379496/71‑1 (MQ=255) gATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCt < 1:3041324/66‑1 (MQ=255) gATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCt < 1:557483/66‑1 (MQ=255) aTAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGGTGGAGCt > 1:764018/1‑71 (MQ=255) aTAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCt < 1:716536/71‑1 (MQ=255) ccATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCAGCGTCTCAAATAATTTGCTGGAGCTGCg > 1:1862300/1‑70 (MQ=255) aTGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc > 1:1627214/1‑45 (MQ=255) aTGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc > 1:1827157/1‑45 (MQ=255) aTGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc > 1:531361/1‑45 (MQ=255) aTGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc > 1:2171138/1‑45 (MQ=255) tGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTg > 1:3297185/1‑56 (MQ=255) tGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTg > 1:1308502/1‑56 (MQ=255) tGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTg > 1:905483/1‑56 (MQ=255) ccAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTgg > 1:2422103/1‑71 (MQ=255) aacagGAAATATACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTa > 1:2592818/1‑71 (MQ=255) aacagGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTa > 1:2588448/1‑71 (MQ=255) aacaaGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTa > 1:273373/1‑71 (MQ=255) caaGAAATAGACGAATATTACGCCCATTTCCGCATCTCAaataa > 1:748045/1‑44 (MQ=255) caaGAAATAGACGAATATTACGCCCATTTCCGCATCTCAaataa > 1:394012/1‑44 (MQ=255) aaaTAGACGAATATTACGCCCATTTCCACATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGtt < 1:1076744/71‑1 (MQ=255) aaTAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTTCGTAATCTGGTACAGGtt < 1:3422933/70‑1 (MQ=255) aTAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTg > 1:2980900/1‑70 (MQ=255) aTAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGc > 1:327896/1‑71 (MQ=255) gACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGa < 1:804734/71‑1 (MQ=255) aaTATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTg < 1:1043925/71‑1 (MQ=255) ttACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGAtt < 1:1983171/70‑1 (MQ=255) ttACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATATGGTACAGGTTGCCGAGTTGATTg > 1:108580/1‑71 (MQ=255) cGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGtt < 1:1080390/70‑1 (MQ=255) gCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCg < 1:1815615/71‑1 (MQ=255) aTTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt < 1:3629290/71‑1 (MQ=255) aTTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt < 1:704719/71‑1 (MQ=255) aTTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt > 1:2590553/1‑71 (MQ=255) tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGc > 1:1450366/1‑70 (MQ=255) ccGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCaa > 1:1394544/1‑71 (MQ=255) cgcaTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAat < 1:1687737/71‑1 (MQ=255) cgcaTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAat < 1:2191954/71‑1 (MQ=255) | CCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAAT > Exported/1744482‑1744619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |