Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,751,256 G→A 52.8% intergenic (‑42/‑165) yfiF ← / → trxC predicted methyltransferase/thioredoxin 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,751,2560GA52.8% 0.9 / 48.4 36intergenic (‑42/‑165)yfiF/trxCpredicted methyltransferase/thioredoxin 2
Reads supporting (aligned to +/- strand):  ref base G (8/9);  new base A (12/7);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGA  >  Exported/1751192‑1751324
                                                                |                                                                    
ttACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTcc                                                                >  1:731719/1‑71 (MQ=255)
 tACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCcg                                                               >  1:395485/1‑71 (MQ=255)
         atttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGtt                                                        <  1:1656647/70‑1 (MQ=255)
          tttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCg                                                      <  1:2380880/71‑1 (MQ=255)
                 gTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGAt                                               >  1:94813/1‑71 (MQ=255)
                    cATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGAttt                                             <  1:2858807/70‑1 (MQ=255)
                        cTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAc                                        >  1:229364/1‑71 (MQ=255)
                           ttCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTAcc                                       >  1:680782/1‑69 (MQ=255)
                               ccaccaGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGtt                                                        >  1:2720768/1‑48 (MQ=255)
                               ccaccaGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTc                                                       >  1:191303/1‑49 (MQ=255)
                               ccaccaGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTc                                                       >  1:1513142/1‑49 (MQ=255)
                                        cTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTAGTACCAAAGCCTGCGACTAt                        <  1:147126/71‑1 (MQ=255)
                                         tGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt                        >  1:2309129/1‑70 (MQ=255)
                                             cgAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATAc                   >  1:3642418/1‑71 (MQ=255)
                                              gAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGc                                  >  1:2565944/1‑55 (MQ=255)
                                              gAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc                              <  1:1732435/59‑1 (MQ=255)
                                              gAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc                              <  1:828747/59‑1 (MQ=255)
                                               aaGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCt                 <  1:2740485/71‑1 (MQ=255)
                                               aaGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCt                 <  1:3288003/71‑1 (MQ=255)
                                                aGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTa                >  1:1124383/1‑71 (MQ=255)
                                                 gcgcgcAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCt                 <  1:3028190/69‑1 (MQ=255)
                                                     gcAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt                     <  1:788859/61‑1 (MQ=255)
                                                     gcAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt                     <  1:760777/61‑1 (MQ=255)
                                                      cAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGaa           >  1:1418955/1‑70 (MQ=255)
                                                      cAGATTACCCAAAGTCCGCGCGGTTCGACATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGaa           >  1:84193/1‑70 (MQ=255)
                                                        gATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGCCTATCATACCTATTGAATa         >  1:2977405/1‑70 (MQ=255)
                                                         aTTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAc                                        <  1:2943929/38‑1 (MQ=255)
                                                         aTTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAc                                        <  1:3367538/38‑1 (MQ=255)
                                                         aTTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAc                                        <  1:2306262/38‑1 (MQ=255)
                                                         aTTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAc                                        <  1:3687355/38‑1 (MQ=255)
                                                         aTTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaa        >  1:2861636/1‑70 (MQ=255)
                                                          ttACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAACGCCTGCGACTATCATACCTATTGAATaaaa      >  1:1220815/1‑71 (MQ=255)
                                                          ttACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa      >  1:3657688/1‑71 (MQ=255)
                                                          ttACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa      >  1:3548457/1‑71 (MQ=255)
                                                           tACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa      >  1:2054966/1‑70 (MQ=255)
                                                              ccGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGa  <  1:2248947/71‑1 (MQ=255)
                                                                |                                                                    
TTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGA  >  Exported/1751192‑1751324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: