| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 218,030 | A→G | 8.7% | Y6C (TAC→TGC) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 218,030 | 0 | A | G | 8.7% | 125.5 / 3.3 | 46 | Y6C (TAC→TGC) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (25/17); new base G (2/2); total (27/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAC > Exported/217960‑218099 | aTCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTa > 1:383285/1‑71 (MQ=255)aTCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTa > 1:3480905/1‑71 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGTACGTGGTAATGAATGAAGACGACTACaaaa > 1:2242711/1‑70 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:1292249/1‑70 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa < 1:3385195/70‑1 (MQ=255) aaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa < 1:2723146/70‑1 (MQ=255) cACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAt > 1:2429495/1‑68 (MQ=255) aCTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATaaga < 1:1578328/71‑1 (MQ=255) cTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGCCGACTACAAAATaag > 1:349178/1‑69 (MQ=255) tGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTa > 1:544938/1‑59 (MQ=255) tGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTa > 1:2053138/1‑59 (MQ=255) gCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAagaaga < 1:3267037/70‑1 (MQ=255) gCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAagag > 1:3335552/1‑71 (MQ=255) cACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAagag > 1:1323149/1‑70 (MQ=255) cACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAagag > 1:2942886/1‑70 (MQ=255) cACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAgg < 1:25306/71‑1 (MQ=255) tGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTa > 1:2129699/1‑52 (MQ=255) gaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGaa > 1:2931290/1‑65 (MQ=255) gaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgca < 1:302336/70‑1 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:1231078/71‑1 (MQ=255) gCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat < 1:880816/71‑1 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACg > 1:2291139/1‑61 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:533800/1‑70 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:530657/1‑70 (MQ=255) gTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTc < 1:1043071/67‑1 (MQ=255) gTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg > 1:2221498/1‑70 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:2323309/1‑71 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatta < 1:2190656/61‑1 (MQ=255) cTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcagca > 1:2149157/1‑54 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:1771386/1‑71 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:2858918/1‑71 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:447723/1‑71 (MQ=255) ggACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGa < 1:2026663/43‑1 (MQ=255) tAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag < 1:114624/42‑1 (MQ=255) tAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAtt > 1:361684/1‑71 (MQ=255) aaatgaatgAAGACGACTACAAAATAAGAAGAGGAAAc > 1:1901886/2‑38 (MQ=255) aaatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGaa < 1:3374404/46‑1 (MQ=255) aatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAc > 1:2683627/1‑62 (MQ=255) aatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTg > 1:304912/1‑71 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTg > 1:418109/1‑70 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:870195/1‑71 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAt < 1:2903562/60‑1 (MQ=255) gacgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTAttt < 1:1918273/42‑1 (MQ=255) gacgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTAttt < 1:2059739/42‑1 (MQ=255) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAatta < 1:809062/37‑1 (MQ=37) gacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATAttt > 1:1931122/1‑70 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt > 1:2770476/1‑71 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt > 1:2775026/1‑71 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTa < 1:1174980/63‑1 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTg < 1:99112/70‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAat > 1:1919138/1‑63 (MQ=255) aCAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:564082/1‑70 (MQ=255) aCAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:988763/1‑70 (MQ=255) | ATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAC > Exported/217960‑218099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |