| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,824,288 | G→A | 54.5% | D452D (GAC→GAT) | hypF ← | carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,824,288 | 0 | G | A | 54.5% | 20.8 / 120.2 | 88 | D452D (GAC→GAT) | hypF | carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
| Reads supporting (aligned to +/- strand): ref base G (27/13); new base A (18/30); total (45/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
AGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCG > Exported/1824223‑1824348 | aGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCTCTGGCTGGAGACATAGCCCGGATGCGCATCATg < 1:939830/71‑1 (MQ=255)aGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATg < 1:1306062/71‑1 (MQ=255)aGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATg < 1:1352859/71‑1 (MQ=255) ttGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGca > 1:2544455/1‑65 (MQ=255) tGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTa > 1:1222878/1‑71 (MQ=255) tGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTa > 1:1649974/1‑71 (MQ=255) gCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTa < 1:3291666/70‑1 (MQ=255) tCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCTTGCACAAc < 1:654133/64‑1 (MQ=255) ggCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCgg > 1:2481099/1‑71 (MQ=255) cAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCgggg > 1:743865/1‑71 (MQ=255) cAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCACGGATGCGCATCATGCACAACGTAt > 1:2724064/1‑64 (MQ=255) gATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGGACAACGTATTGCGGGGTa < 1:2595544/71‑1 (MQ=255) aTTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGt < 1:3083935/69‑1 (MQ=255) cATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTAt < 1:2112227/58‑1 (MQ=255) aTTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATc < 1:2357117/71‑1 (MQ=255) aTTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGAGGGGTAAAATc < 1:526034/71‑1 (MQ=255) tttCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCa < 1:297810/44‑1 (MQ=255) tttCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTg > 1:1024323/1‑58 (MQ=255) tttCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTg > 1:764486/1‑58 (MQ=255) tttCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTg > 1:1952831/1‑58 (MQ=255) tttCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCATCATCCACAACGTATTg > 1:1194438/1‑58 (MQ=255) tCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCg > 1:3145744/1‑69 (MQ=255) aCGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCg > 1:509301/1‑67 (MQ=255) aCGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCg > 1:851598/1‑67 (MQ=255) aCGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTa < 1:2763131/69‑1 (MQ=255) cgcCCACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCg < 1:909990/64‑1 (MQ=255) gcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGtt > 1:343642/1‑71 (MQ=255) cccACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt < 1:2408197/71‑1 (MQ=255) ccACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGtttt > 1:2838259/1‑71 (MQ=255) ccACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGtttt < 1:939539/71‑1 (MQ=255) ccACTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGtttt < 1:2916342/71‑1 (MQ=255) aCTGGCTGGAGACATAGCCCGTATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTg > 1:3296548/1‑70 (MQ=255) aCTGGCTGGAGACATAGCCCGGATGCGCGTCATGc < 1:538659/35‑1 (MQ=255) aCTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTa > 1:2348441/1‑43 (MQ=255) aCTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTg > 1:54973/1‑70 (MQ=255) aCTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGtttt > 1:3624743/1‑69 (MQ=255) aCTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGtttt > 1:1004317/1‑69 (MQ=255) aCTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTg > 1:3031115/1‑70 (MQ=255) aCTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTg > 1:550794/1‑70 (MQ=255) aCTGGCTGGAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTg > 1:2161356/1‑70 (MQ=255) ctggctggAGACATAGCCCGGATGCGCATCATGCACAACg < 1:2861382/40‑1 (MQ=255) ctggctggAGACATAGCCCGGATGCGCATCATGCACAACg < 1:605682/40‑1 (MQ=255) tggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGtttt < 1:407830/67‑1 (MQ=255) ggctggAGACATAGCCCGGATGCGCGTCATGCACAAc > 1:2917736/1‑37 (MQ=255) ggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGc > 1:30468/1‑44 (MQ=255) ggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGtttt < 1:3653981/66‑1 (MQ=255) ggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt > 1:1713364/1‑70 (MQ=255) ggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt > 1:3130176/1‑70 (MQ=255) ggctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt > 1:837470/1‑70 (MQ=255) gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGc > 1:2813750/1‑67 (MQ=255) gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGc > 1:2396969/1‑67 (MQ=255) gctggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGc > 1:3629819/1‑67 (MQ=255) tggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt > 1:647456/1‑62 (MQ=255) tggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt > 1:1240828/1‑62 (MQ=255) tggAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGttt > 1:144015/1‑62 (MQ=255) tggAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGt < 1:2339049/54‑1 (MQ=255) tggAGACATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGt < 1:3284054/54‑1 (MQ=255) gagaCATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCAt < 1:2630556/65‑1 (MQ=255) gaCATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGt > 1:3599406/1‑70 (MQ=255) gaCATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGt > 1:1570354/1‑70 (MQ=255) cATAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAAc < 1:58240/71‑1 (MQ=255) aTAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACg < 1:3472775/71‑1 (MQ=255) aTAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACg < 1:3477221/71‑1 (MQ=255) aTAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACg < 1:266633/71‑1 (MQ=255) aTAGCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACg < 1:2544741/71‑1 (MQ=255) tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt < 1:2860058/50‑1 (MQ=255) tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt < 1:1175628/50‑1 (MQ=255) tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt < 1:2102799/50‑1 (MQ=255) tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt < 1:2436431/50‑1 (MQ=255) tAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt < 1:3567778/50‑1 (MQ=255) aGCCCGGAAGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAt > 1:1862285/1‑49 (MQ=255) gCCCGGATGGGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCtt > 1:1401666/1‑71 (MQ=255) gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCg > 1:1097726/1‑43 (MQ=255) gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCg > 1:305699/1‑43 (MQ=255) gCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCg > 1:236269/1‑43 (MQ=255) gCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCtt < 1:3083377/71‑1 (MQ=255) gCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCtt < 1:2940334/71‑1 (MQ=255) gCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCtt < 1:1854990/71‑1 (MQ=255) gCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCtt > 1:2361926/1‑71 (MQ=255) gCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCtt > 1:194431/1‑71 (MQ=255) gCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCt < 1:1944942/70‑1 (MQ=255) gCCCGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATAGTAGATGTTTTGCATCa > 1:3520061/1‑59 (MQ=39) cccGGATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTc < 1:1115757/71‑1 (MQ=255) gATGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAAc < 1:3291801/62‑1 (MQ=255) aTGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTcg > 1:3310277/1‑67 (MQ=255) tGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGt < 1:3119100/57‑1 (MQ=255) tGCGCATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGt < 1:326810/57‑1 (MQ=255) gcgcATCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGt < 1:2394053/56‑1 (MQ=255) gcgTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTgt < 1:1605857/45‑2 (MQ=255) gcgTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGc < 1:3605173/45‑1 (MQ=255) gcgTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGc < 1:2048358/45‑1 (MQ=255) | AGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCG > Exported/1824223‑1824348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |