BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,878,756	G→A	12.9%	G131G (GGC→GGT)	pyrG ←	CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,878,756	0	G	A	12.9%	387.2 / 37.9	139	G131G (GGC→GGT)	pyrG	CTP synthetase
Reads supporting (aligned to +/- strand): ref base G (64/57); new base A (8/10); total (72/67)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

AGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGG  >  Exported/1878688‑1878825

aGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGcc                                                                     <  1:3403543/71‑1 (MQ=255)

 ggAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCa                                                                    >  1:3717783/1‑71 (MQ=255)

 ggAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCa                                                                    >  1:2820899/1‑71 (MQ=255)

  gAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCg                                                                       <  1:3319548/67‑1 (MQ=255)

      ggCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTc                                                               <  1:742972/71‑1 (MQ=255)

      ggCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCACCACCTTc                                                               <  1:2202295/71‑1 (MQ=255)

       gCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTcc                                                              <  1:2247489/71‑1 (MQ=255)

        cAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCa                                                             <  1:1156958/71‑1 (MQ=255)

         aaGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAg                                                            >  1:1598479/1‑71 (MQ=255)

             aTTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg                                                        >  1:3224204/1‑71 (MQ=255)

              ttCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCACCACCTTCCAGCAcgc                                                       >  1:2842673/1‑71 (MQ=255)

                cGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg                                                        >  1:524513/1‑68 (MQ=255)

                cGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg                                                        >  1:1284684/1‑68 (MQ=255)

                cGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACgcgc                                                     >  1:889997/1‑71 (MQ=255)

                cGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACgcgc                                                     >  1:2454345/1‑71 (MQ=255)

                 gATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTcc                                                              >  1:2606400/1‑61 (MQ=255)

                 gATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCa                                                             >  1:1810207/1‑62 (MQ=255)

                 gATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGct                                                    <  1:2984934/71‑1 (MQ=255)

                 gATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGct                                                    <  1:2996079/71‑1 (MQ=255)

                 gATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGct                                                    <  1:2586852/71‑1 (MQ=255)

                   tatCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGctct                                                  <  1:2286428/71‑1 (MQ=255)

                    atCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCACCACCTTCCa                                                             >  1:1724298/1‑59 (MQ=255)

                    atCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCACCACCTTCCAGGACGCGCTCtt                                                 >  1:1845950/1‑71 (MQ=255)

                     tCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCtt                                                 <  1:1303637/70‑1 (MQ=255)

                       aCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGa                                              <  1:3108018/71‑1 (MQ=255)

                         cTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGAtt                                            >  1:1356057/1‑71 (MQ=255)

                             tGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGt                                        >  1:257329/1‑71 (MQ=255)

                               tACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCtt                                                 >  1:3531978/1‑60 (MQ=255)

                               tACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCtt                                                 >  1:2295224/1‑60 (MQ=255)

                               tACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGt                                        >  1:2978481/1‑69 (MQ=255)

                               tACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGt                                        >  1:1051603/1‑69 (MQ=255)

                               tACCGCCGATTTCTACCAGTACTACGTCATGACCTTCACCACCTTCCAGCACGCGCTCTTTGATTGCGt                                        <  1:2884755/69‑1 (MQ=255)

                               tACCGCCGATTTCTACCAGTACTACGTCATGACCTTCACCACCTTCCAGCACGCGCTCTTTGATTGCGTTa                                      <  1:1022688/71‑1 (MQ=255)

                                aCCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAg                                     >  1:1156094/1‑71 (MQ=255)

                                 ccgccgATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGt                                    <  1:1933996/71‑1 (MQ=255)

                                 ccgccgATTTCTACCAGTACTACGTCATGACCTTCACCACCTTcc                                                              <  1:2620154/45‑1 (MQ=255)

                                 ccgccgATTTCTACCAGTACTACGTCATGACCTTCACCACCTTcc                                                              <  1:2289322/45‑1 (MQ=255)

                                      gATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg                                                        <  1:1584636/46‑1 (MQ=255)

                                      gATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtgt                               >  1:3022094/1‑71 (MQ=255)

                                      gATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtgt                               >  1:297492/1‑71 (MQ=255)

                                      gATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtgt                               >  1:3607428/1‑71 (MQ=255)

                                      gATGTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtgt                               >  1:1337238/1‑71 (MQ=255)

                                       aTTTCTACCAGTACTACGTCATGACCTTCGCCACCTTcc                                                              <  1:3377787/39‑1 (MQ=255)

                                       aTTTCTACCAGTACTACGTCATGACCTTCGCCACCTTcc                                                              <  1:2312094/39‑1 (MQ=255)

                                       aTTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtgtg                              >  1:1066216/1‑71 (MQ=255)

                                       aTTTCTACCAGTACTACGACATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtgtg                              >  1:1257207/1‑71 (MQ=255)

                                        tttCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTg                                   <  1:1448068/65‑1 (MQ=255)

                                        tttCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGc                             <  1:3014935/71‑1 (MQ=255)

                                        tttCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGc                             <  1:3615555/71‑1 (MQ=255)

                                         ttCTACCAGTACTACGTCATGACCTTCGCCACCtt                                                                >  1:1274881/1‑35 (MQ=255)

                                         ttCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAg                                                            >  1:2872925/1‑39 (MQ=255)

                                         ttCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg                                                        <  1:1534425/43‑1 (MQ=255)

                                         ttCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGAtt                                            >  1:2791566/1‑55 (MQ=255)

                                         ttCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCg                            >  1:1999708/1‑71 (MQ=255)

                                          tCTACCAGTACTACGTCATGACCTTCGCCACCTTCAAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCg                            >  1:3463701/1‑70 (MQ=255)

                                            tACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACgcgc                                                     <  1:83873/43‑1 (MQ=255)

                                            tACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACgcgc                                                     <  1:231161/43‑1 (MQ=255)

                                            tACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGtt                                       <  1:2113138/57‑1 (MQ=255)

                                            tACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGtt                                       <  1:3078583/57‑1 (MQ=255)

                                            tACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGtt                                       <  1:2367645/57‑1 (MQ=255)

                                             aCCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGAGGaa                         >  1:982425/1‑70 (MQ=255)

                                              ccAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAg                                     >  1:3327554/1‑57 (MQ=255)

                                              ccAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGaa                         <  1:369850/69‑1 (MQ=255)

                                              ccAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGa                          >  1:785497/1‑68 (MQ=255)

                                              ccAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAAt                        >  1:1565245/1‑70 (MQ=255)

                                              ccAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAAt                        >  1:781181/1‑70 (MQ=255)

                                              ccAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATa                       <  1:3616443/71‑1 (MQ=255)

                                              ccAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATa                       <  1:1749440/71‑1 (MQ=255)

                                                 gTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAAcc                    >  1:1384000/1‑71 (MQ=255)

                                                 gTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAAcc                    >  1:3513565/1‑71 (MQ=255)

                                                   actacGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtgt                               >  1:1099097/1‑58 (MQ=255)

                                                   actacGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTg                  >  1:2144485/1‑71 (MQ=255)

                                                     tacGTCATGACCTTCGCCACCTTCCAGCACGCGCTCttt                                                <  1:563221/39‑1 (MQ=255)

                                                     tacGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATa                       <  1:902740/64‑1 (MQ=255)

                                                     tacGTCATGACCTTCACCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCa                >  1:584855/1‑71 (MQ=255)

                                                        gTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGGTAGTGATGTGCGGAATAACCTGCACgg             <  1:67355/71‑1 (MQ=255)

                                                          cATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCg                                         >  1:3499635/1‑41 (MQ=255)

                                                          cATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGt                                        >  1:1003302/1‑42 (MQ=255)

                                                            tGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTgc         >  1:2988328/1‑71 (MQ=255)

                                                            tGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTgc         >  1:447603/1‑71 (MQ=255)

                                                                cTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGt            <  1:3464135/64‑1 (MQ=255)

                                                                cTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCg     <  1:2902666/71‑1 (MQ=255)

                                                                cTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCg     <  1:2194579/71‑1 (MQ=255)

                                                                cTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTCGCGCCg     <  1:2046/71‑1 (MQ=255)

                                                                 ttCACCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGcc      >  1:552983/1‑69 (MQ=255)

                                                                  tCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGt                                    >  1:1377847/1‑38 (MQ=255)

                                                                  tCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGt                                    >  1:391526/1‑38 (MQ=255)

                                                                  tCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGt                                    >  1:646973/1‑38 (MQ=255)

                                                                  tCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGa                                  >  1:3151107/1‑40 (MQ=255)

                                                                  tCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTg          >  1:2174685/1‑64 (MQ=255)

                                                                  tCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTg          >  1:1290856/1‑64 (MQ=255)

                                                                  tCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTg          >  1:1850809/1‑64 (MQ=255)

                                                                  tCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTg          >  1:3542706/1‑64 (MQ=255)

                                                                  tCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAg   >  1:1723157/1‑71 (MQ=255)

                                                                   cGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGAtg                                >  1:2239299/1‑41 (MQ=255)

                                                                   cGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGAGATGTGCGGAATAACCTGCACGGTTGCGCCGa    <  1:2572612/69‑1 (MQ=255)

                                                                    gCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGa    <  1:1965402/68‑1 (MQ=255)

                                                                    gCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGa    <  1:1686605/68‑1 (MQ=255)

                                                                    gCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGa    <  1:1642787/68‑1 (MQ=255)

                                                                    gCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAgg  <  1:3160385/70‑1 (MQ=255)

AGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGG  >  Exported/1878688‑1878825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`