| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 218,049 | C→T | 9.1% | N12N (AAC→AAT) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 218,049 | 0 | C | T | 9.1% | 165.1 / 6.3 | 55 | N12N (AAC→AAT) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (33/17); new base T (2/3); total (35/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01 | |||||||||||
GAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCA > Exported/217983‑218116 | gaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgca < 1:302336/70‑1 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGcagcag < 1:1231078/71‑1 (MQ=255) gCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAat < 1:880816/71‑1 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACg > 1:2291139/1‑61 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:533800/1‑70 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:530657/1‑70 (MQ=255) gTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTc < 1:1043071/67‑1 (MQ=255) gTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAgg > 1:2221498/1‑70 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGa > 1:2323309/1‑71 (MQ=255) tCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatta < 1:2190656/61‑1 (MQ=255) cTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcagca > 1:2149157/1‑54 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:2858918/1‑71 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:447723/1‑71 (MQ=255) tGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATa > 1:1771386/1‑71 (MQ=255) tAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag < 1:114624/42‑1 (MQ=255) tAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACAtt > 1:361684/1‑71 (MQ=255) aaatgaatgAAGACGACTACAAAATAAGAAGAGGAAAc > 1:1901886/2‑38 (MQ=255) aaatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGaa < 1:3374404/46‑1 (MQ=255) aatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAc > 1:2683627/1‑62 (MQ=255) aatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTg > 1:304912/1‑71 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTg > 1:418109/1‑70 (MQ=255) atgaatgaAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGc > 1:870195/1‑71 (MQ=255) aGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAt < 1:2903562/60‑1 (MQ=255) gacgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTAttt < 1:2059739/42‑1 (MQ=255) gacgacTACAAAATAAGAAGAGGAAACGCAGCAGAATTAttt < 1:1918273/42‑1 (MQ=255) cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAatta < 1:809062/37‑1 (MQ=37) gacTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATAttt > 1:1931122/1‑70 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt > 1:2775026/1‑71 (MQ=255) gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtttt > 1:2770476/1‑71 (MQ=255) acTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTa < 1:1174980/63‑1 (MQ=255) ctgcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa > 1:3232581/4‑71 (MQ=255) cTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTg < 1:99112/70‑1 (MQ=255) tACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAat > 1:1919138/1‑63 (MQ=255) aCAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:988763/1‑70 (MQ=255) aCAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc > 1:564082/1‑70 (MQ=255) aaaTAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:2034124/1‑35 (MQ=255) aaaTAAGAAGAGGAAACGCAGCAGAATTATTTTCa > 1:3544056/1‑35 (MQ=255) aaTAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt > 1:2258372/1‑70 (MQ=255) aaTAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt > 1:1186757/1‑70 (MQ=255) aaTAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATg < 1:2824569/71‑1 (MQ=255) tAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgag < 1:3443837/71‑1 (MQ=255) agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTaa < 1:1975509/53‑1 (MQ=255) aagaGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:277144/71‑1 (MQ=255) agagGAAACGCAGCAGAATTATTTTCAGGGATACGTCACATTGCTATTAATATTTTGACGAATGAGAAgg < 1:3413778/70‑1 (MQ=255) ggAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAtt > 1:2187636/1‑71 (MQ=255) ggAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAt > 1:2769298/1‑70 (MQ=255) gAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAtt > 1:1591808/1‑70 (MQ=255) aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTg > 1:3228906/1‑38 (MQ=255) aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTg > 1:3127976/1‑38 (MQ=255) aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAt < 1:688660/68‑1 (MQ=255) aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCa < 1:803445/71‑1 (MQ=255) aaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGaa > 1:3737457/1‑62 (MQ=255) aaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc > 1:345098/1‑69 (MQ=255) aaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc > 1:2253703/1‑69 (MQ=255) aaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCa > 1:1010457/1‑70 (MQ=255) aCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTAtt > 1:1661006/1‑67 (MQ=255) | GAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCA > Exported/217983‑218116 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |