Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,927,871 T→C 13.9% K450R (AAA→AGA)  araE ← arabinose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,927,8710TC13.9% 246.4 / 27.7 95K450R (AAA→AGA) araEarabinose transporter
Reads supporting (aligned to +/- strand):  ref base T (39/42);  new base C (8/5);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACG  >  Exported/1927801‑1927940
                                                                      |                                                                     
tCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtttt                                                                       <  1:2520997/71‑1 (MQ=255)
        cGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTcc                                                               <  1:588279/71‑1 (MQ=255)
          atatTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCgg                                                             <  1:1805512/71‑1 (MQ=255)
           tatTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCgg                                                             >  1:547438/1‑70 (MQ=255)
              ttCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAAt                                                          >  1:2827329/1‑70 (MQ=255)
                 tcAACTTCTCGCCTGCCTTCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTATGGTTTCCGGAATGAGc                                                      >  1:2933423/1‑71 (MQ=255)
                   aaCTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGa                                                            >  1:3606371/1‑63 (MQ=255)
                   aaCTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGc                                                      <  1:3062141/69‑1 (MQ=255)
                   aaCTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGc                                                      >  1:426737/1‑69 (MQ=255)
                     cTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGa                                                  <  1:452167/71‑1 (MQ=255)
                       tctcGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAAAGAGCCAGa                                                  >  1:77919/1‑69 (MQ=255)
                        ctcGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGaaa                                                <  1:3167901/70‑1 (MQ=255)
                        ctcGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTCTGGTTTCCGGAATGAGCCAGaa                                                 <  1:1263220/69‑1 (MQ=255)
                        ctcGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTCTGGTTTCCGGAATGAGCCAGaa                                                 <  1:1905991/69‑1 (MQ=255)
                         tcGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAg                                               >  1:1121639/1‑70 (MQ=255)
                         tcGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAg                                               >  1:1098087/1‑70 (MQ=255)
                          cGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTCTGGTTTCCGGAATGAGCCAGaa                                                 <  1:928204/67‑1 (MQ=255)
                            ccTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAAt                                           <  1:2484800/71‑1 (MQ=255)
                            ccTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAAt                                           <  1:1616532/71‑1 (MQ=255)
                            ccTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTCTGGTTTCCGGAATGAGCCAGAAAGTAAt                                           >  1:2088160/1‑71 (MQ=255)
                            ccTGCCATCAGTTTGCGTTCGATATGTTACAGCGTGACATTTCTGGTTTCCGGAATGAGCCAGAAAGTAAt                                           >  1:1394892/1‑71 (MQ=255)
                              tGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGa                                                  <  1:425375/62‑1 (MQ=255)
                              tGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGa                                                  <  1:2109820/62‑1 (MQ=255)
                                ccATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGcc                                        >  1:3705202/1‑70 (MQ=255)
                                 cATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtttt                                                                       >  1:2224650/1‑38 (MQ=255)
                                 cATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                           <  1:1975497/50‑1 (MQ=255)
                                 cATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                           <  1:601939/50‑1 (MQ=255)
                                  aTCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCca                                      >  1:1033025/1‑70 (MQ=255)
                                   tCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATg                                                         >  1:2571206/1‑50 (MQ=255)
                                   tCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGa                                                  <  1:977805/57‑1 (MQ=255)
                                   tCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGa                                                  <  1:403349/57‑1 (MQ=255)
                                   tCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGa                                                  <  1:535757/57‑1 (MQ=255)
                                       tttGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCgg                                                             <  1:3162257/42‑1 (MQ=255)
                                       tttGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTaa                                            >  1:2976791/1‑59 (MQ=255)
                                       tttGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTaa                                            >  1:1805709/1‑59 (MQ=255)
                                       tttGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACg                                <  1:962127/71‑1 (MQ=255)
                                        ttGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCgg                                                             <  1:978630/41‑1 (MQ=255)
                                         tGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                           >  1:3674905/1‑42 (MQ=255)
                                         tGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                           >  1:3007429/1‑42 (MQ=255)
                                         tGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                           >  1:1083493/1‑42 (MQ=255)
                                         tGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                                           >  1:3619952/1‑42 (MQ=255)
                                         tGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGcc                                                     >  1:1079104/1‑48 (MQ=255)
                                         tGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGc                               <  1:2145800/70‑1 (MQ=255)
                                            gTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGa                                                        <  1:1339683/42‑1 (MQ=255)
                                            gTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATg                           <  1:1603902/71‑1 (MQ=255)
                                             ttCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGt                                              <  1:1396682/51‑1 (MQ=255)
                                             ttCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGt                          >  1:591082/1‑71 (MQ=255)
                                              tCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGc                                                      >  1:1205308/1‑42 (MQ=255)
                                              tCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGc                                                      >  1:281755/1‑42 (MQ=255)
                                              tCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGc                                                      >  1:309039/1‑42 (MQ=255)
                                              tCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGtt                         >  1:3410629/1‑71 (MQ=255)
                                               agATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGtt                         >  1:3624714/2‑70 (MQ=255)
                                                gATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGa                                                  <  1:318013/44‑1 (MQ=255)
                                                 atatGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAg                      >  1:317069/1‑71 (MQ=255)
                                                    tGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCa                       <  1:1327799/67‑1 (MQ=255)
                                                    tGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAgcg                    >  1:2115921/1‑70 (MQ=255)
                                                      ttCCAGCGTGACATTTCTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAg                 <  1:946137/71‑1 (MQ=255)
                                                       tCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGaaa                                                >  1:396912/1‑39 (MQ=255)
                                                       tCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGaaa                                                >  1:3009586/1‑39 (MQ=255)
                                                       tCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGaaa                                                >  1:3019310/1‑39 (MQ=255)
                                                       tCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAgt                >  1:2741682/1‑71 (MQ=255)
                                                        ccAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAAt                                           <  1:2855685/43‑1 (MQ=255)
                                                        ccAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAAt                                           <  1:172065/43‑1 (MQ=255)
                                                        ccAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAAt                                           <  1:2416594/43‑1 (MQ=255)
                                                         cAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAgtgt              <  1:3171711/71‑1 (MQ=255)
                                                          aGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGc                                         >  1:790134/1‑43 (MQ=255)
                                                          aGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGc                                         >  1:3075325/1‑43 (MQ=255)
                                                          aGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCcaca                                    <  1:3654641/48‑1 (MQ=255)
                                                          aGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCcaca                                    <  1:3249104/48‑1 (MQ=255)
                                                          aGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCcaca                                    <  1:429224/48‑1 (MQ=255)
                                                           gCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAAc                                 >  1:3065281/1‑50 (MQ=255)
                                                           gCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAAc                                 >  1:1764082/1‑50 (MQ=255)
                                                           gCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAgtg               >  1:634904/1‑68 (MQ=255)
                                                           gCGTGACATTTCTGGTTTCCGGAATGAGCCAGAAAGTAATGCCcaca                                    <  1:1772655/47‑1 (MQ=255)
                                                            cGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTat                                            <  1:145966/38‑2 (MQ=255)
                                                            cGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTaga           <  1:519590/71‑1 (MQ=255)
                                                            cGTGACATTTCTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTa             >  1:2828361/1‑69 (MQ=255)
                                                              tGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGc         >  1:3268221/1‑71 (MQ=255)
                                                               taCATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGtt                         <  1:3623675/53‑1 (MQ=255)
                                                                aCATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGc         >  1:469391/1‑69 (MQ=255)
                                                                 cttttttGGTTTCCGGAATGAGCCAGAAAGTAATGCCcaca                                    <  1:3534879/39‑1 (MQ=255)
                                                                 cATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCa                       >  1:3213880/1‑54 (MQ=255)
                                                                 cATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAg      <  1:1748350/71‑1 (MQ=255)
                                                                 cATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAg      <  1:949660/71‑1 (MQ=255)
                                                                 cATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAg      <  1:3337334/71‑1 (MQ=255)
                                                                 cATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAg      <  1:3587524/71‑1 (MQ=255)
                                                                  aTTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAg      >  1:456927/1‑70 (MQ=255)
                                                                  aTTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGa     <  1:2144165/71‑1 (MQ=255)
                                                                  aTTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGa     <  1:1239989/71‑1 (MQ=255)
                                                                  aTTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGa     <  1:2361263/71‑1 (MQ=255)
                                                                  aTTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGa     <  1:16975/71‑1 (MQ=255)
                                                                  aTTTCTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAAc                                 >  1:3704957/1‑43 (MQ=255)
                                                                  aTTTCTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAg      >  1:3032463/1‑70 (MQ=255)
                                                                  aTTTCTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAg      >  1:3362903/1‑70 (MQ=255)
                                                                  aTTTCTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAg      >  1:530385/1‑70 (MQ=255)
                                                                  aTTTCTGGTTTCCGGAATGAGCCAGAAAGTAATGCCAACAAACGCAATGTTCAGCGCAGTGTAGAGCCAg      >  1:493546/1‑70 (MQ=255)
                                                                     tttGGTTTCCGGAATGAGCCAGAAAGTAATGCCcaca                                    >  1:3442509/1‑37 (MQ=255)
                                                                     tttGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAAc   <  1:184335/70‑1 (MQ=255)
                                                                     tttGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAAc   <  1:371002/70‑1 (MQ=255)
                                                                     tttGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACg  >  1:1768797/1‑71 (MQ=255)
                                                                      |                                                                     
TCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTAGAGCCAGAACG  >  Exported/1927801‑1927940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: