Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,933,228 G→A 10.3% D405D (GAC→GAT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,933,2280GA10.3% 168.4 / 8.6 58D405D (GAC→GATlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (20/32);  new base A (2/4);  total (22/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTG  >  Exported/1933159‑1933298
                                                                     |                                                                      
gCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcg                                                                        <  1:2922125/70‑1 (MQ=255)
     aGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTa                                                                  <  1:2763318/71‑1 (MQ=255)
     aGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTa                                                                  <  1:1966564/71‑1 (MQ=255)
      gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtcgt                                                                    <  1:917838/68‑1 (MQ=255)
        cGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTa                                                                  <  1:2802648/68‑1 (MQ=255)
        cGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTa                                                                  <  1:3720848/68‑1 (MQ=255)
        cGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTa                                                                  <  1:2411426/68‑1 (MQ=255)
        cGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTa                                                                  <  1:596875/68‑1 (MQ=255)
         gTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacgac                                                              <  1:7944/71‑1 (MQ=255)
         gTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacga                                                               >  1:1156118/1‑70 (MQ=255)
         gTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacga                                                               >  1:2877869/1‑70 (MQ=255)
            aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc                                                            <  1:1211217/70‑1 (MQ=255)
              cGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc                                                          >  1:797113/1‑70 (MQ=255)
                aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacgac                                                              >  1:63579/1‑64 (MQ=255)
                aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg                                                       >  1:1621589/1‑71 (MQ=255)
                aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg                                                       <  1:3030350/71‑1 (MQ=255)
                aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg                                                       <  1:3266250/71‑1 (MQ=255)
                 tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc                                                          >  1:3372925/1‑67 (MQ=255)
                 tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg                                                       <  1:2259486/70‑1 (MQ=255)
                 tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc                                                      <  1:413429/71‑1 (MQ=255)
                   tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg                                                     <  1:638612/70‑1 (MQ=255)
                    cACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc                                                         >  1:1015024/1‑65 (MQ=255)
                      cGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGggga                                                 >  1:2921249/1‑71 (MQ=255)
                      cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga                                                 <  1:700539/71‑1 (MQ=255)
                       gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga                                                 <  1:3321789/70‑1 (MQ=255)
                       gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaa                                                <  1:2394561/71‑1 (MQ=255)
                        accaccAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaaa                                               >  1:1221087/1‑71 (MQ=255)
                        accaccAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaa                                                <  1:1241886/70‑1 (MQ=255)
                          caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAATCt                                             <  1:273048/71‑1 (MQ=255)
                           accaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacg                                                                <  1:2782776/51‑1 (MQ=255)
                             caATGAAGCACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt                                          >  1:2549195/1‑71 (MQ=255)
                                tGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacgac                                                              <  1:2036788/48‑1 (MQ=255)
                                   agaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt                                            <  1:2271152/63‑1 (MQ=255)
                                    gaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc                                                            >  1:2138673/1‑46 (MQ=255)
                                    gaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc                                                            >  1:1784565/1‑46 (MQ=255)
                                    gaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc                                                            >  1:1741593/1‑46 (MQ=255)
                                      aCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata                                  <  1:525533/70‑1 (MQ=255)
                                         cAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATAtt                                >  1:1097369/1‑69 (MQ=255)
                                          aaaGCGGTCTGTGATTTCCGGGTTAACGTCGTTac                                                                 >  1:1437935/1‑35 (MQ=255)
                                           aaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc                                              <  1:466910/53‑1 (MQ=255)
                                           aaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc                                              <  1:471292/53‑1 (MQ=255)
                                            aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt                                            >  1:1379386/1‑54 (MQ=255)
                                            aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt                                            >  1:2519784/1‑54 (MQ=255)
                                              cGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAAt                         <  1:3339145/71‑1 (MQ=255)
                                                gTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGa                       <  1:704248/71‑1 (MQ=255)
                                                 tCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCa                              <  1:2701564/63‑1 (MQ=255)
                                                       aTTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTaa                          <  1:73438/61‑1 (MQ=255)
                                                       aTTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGttg                 >  1:554603/1‑68 (MQ=255)
                                                          tCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCt              >  1:3422507/1‑70 (MQ=255)
                                                           ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg             <  1:5978/70‑1 (MQ=255)
                                                           ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg             <  1:1115944/70‑1 (MQ=255)
                                                           ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg             <  1:1139038/70‑1 (MQ=255)
                                                            cGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGaa           >  1:2494362/1‑71 (MQ=255)
                                                            cGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGa            <  1:862675/70‑1 (MQ=255)
                                                              ggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt          >  1:3521032/1‑70 (MQ=255)
                                                               gTTATCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa        <  1:301384/71‑1 (MQ=255)
                                                               gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg                                      >  1:927109/1‑41 (MQ=255)
                                                                ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa                           <  1:451700/51‑1 (MQ=255)
                                                                ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGa            <  1:3208918/66‑1 (MQ=255)
                                                                   aCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt     <  1:1666789/70‑1 (MQ=255)
                                                                     gtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgtg  >  1:741130/1‑71 (MQ=255)
                                                                     |                                                                      
GCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTG  >  Exported/1933159‑1933298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: