Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,933,240 C→T 12.5% A401A (GCG→GCA lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,933,2400CT12.5% 128.4 / 8.6 48A401A (GCG→GCAlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (17/25);  new base T (2/4);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAC  >  Exported/1933171‑1933308
                                                                     |                                                                    
aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc                                                                      <  1:1211217/70‑1 (MQ=255)
  cGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc                                                                    >  1:797113/1‑70 (MQ=255)
    aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg                                                                 >  1:1621589/1‑71 (MQ=255)
    aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg                                                                 <  1:3030350/71‑1 (MQ=255)
    aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg                                                                 <  1:3266250/71‑1 (MQ=255)
     tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc                                                                    >  1:3372925/1‑67 (MQ=255)
     tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg                                                                 <  1:2259486/70‑1 (MQ=255)
     tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc                                                                <  1:413429/71‑1 (MQ=255)
       tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg                                                               <  1:638612/70‑1 (MQ=255)
        cACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc                                                                   >  1:1015024/1‑65 (MQ=255)
          cGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGggga                                                           >  1:2921249/1‑71 (MQ=255)
          cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga                                                           <  1:700539/71‑1 (MQ=255)
           gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga                                                           <  1:3321789/70‑1 (MQ=255)
           gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaa                                                          <  1:2394561/71‑1 (MQ=255)
            accaccAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaaa                                                         >  1:1221087/1‑71 (MQ=255)
            accaccAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaa                                                          <  1:1241886/70‑1 (MQ=255)
              caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAATCt                                                       <  1:273048/71‑1 (MQ=255)
                 caATGAAGCACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt                                                    >  1:2549195/1‑71 (MQ=255)
                       agaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt                                                      <  1:2271152/63‑1 (MQ=255)
                        gaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc                                                                      >  1:1784565/1‑46 (MQ=255)
                        gaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc                                                                      >  1:2138673/1‑46 (MQ=255)
                        gaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc                                                                      >  1:1741593/1‑46 (MQ=255)
                          aCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata                                            <  1:525533/70‑1 (MQ=255)
                             cAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATAtt                                          >  1:1097369/1‑69 (MQ=255)
                               aaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc                                                        <  1:471292/53‑1 (MQ=255)
                               aaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc                                                        <  1:466910/53‑1 (MQ=255)
                                aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt                                                      >  1:2519784/1‑54 (MQ=255)
                                aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt                                                      >  1:1379386/1‑54 (MQ=255)
                                  cGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAAt                                   <  1:3339145/71‑1 (MQ=255)
                                    gTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGa                                 <  1:704248/71‑1 (MQ=255)
                                     tCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCa                                        <  1:2701564/63‑1 (MQ=255)
                                           aTTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTaa                                    <  1:73438/61‑1 (MQ=255)
                                           aTTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGttg                           >  1:554603/1‑68 (MQ=255)
                                              tCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCt                        >  1:3422507/1‑70 (MQ=255)
                                               ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg                       <  1:5978/70‑1 (MQ=255)
                                               ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg                       <  1:1139038/70‑1 (MQ=255)
                                               ccGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTg                       <  1:1115944/70‑1 (MQ=255)
                                                cGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGaa                     >  1:2494362/1‑71 (MQ=255)
                                                cGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGa                      <  1:862675/70‑1 (MQ=255)
                                                  ggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt                    >  1:3521032/1‑70 (MQ=255)
                                                   gTTATCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa                  <  1:301384/71‑1 (MQ=255)
                                                   gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg                                                >  1:927109/1‑41 (MQ=255)
                                                    ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa                                     <  1:451700/51‑1 (MQ=255)
                                                    ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGa                      <  1:3208918/66‑1 (MQ=255)
                                                       aCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt               <  1:1666789/70‑1 (MQ=255)
                                                         gtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgtg            >  1:741130/1‑71 (MQ=255)
                                                          tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa                                     <  1:2809988/45‑1 (MQ=255)
                                                          tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc           >  1:3219514/1‑71 (MQ=255)
                                                          tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc           >  1:2874648/1‑71 (MQ=255)
                                                          tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTCAATCAGATGTGc           >  1:2118866/1‑71 (MQ=255)
                                                               acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt         <  1:2891770/68‑1 (MQ=255)
                                                               acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt         <  1:1248812/68‑1 (MQ=255)
                                                               acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTg      >  1:1541410/1‑71 (MQ=255)
                                                                cgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCt                        >  1:2138303/1‑52 (MQ=255)
                                                                   cgcgCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc  >  1:1590119/1‑71 (MQ=255)
                                                                    gcgcCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGc     <  1:1110939/67‑1 (MQ=255)
                                                                     cgcCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTg                       <  1:1172822/48‑1 (MQ=255)
                                                                     |                                                                    
ACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAC  >  Exported/1933171‑1933308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: