| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,970,934 | C→T | 8.0% | E658K (GAG→AAG) | speA ← | biosynthetic arginine decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,970,934 | 0 | C | T | 8.0% | 233.0 / 8.3 | 75 | E658K (GAG→AAG) | speA | biosynthetic arginine decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base C (26/43); new base T (3/3); total (29/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.52e-01 | |||||||||||
TGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTT > Exported/1970867‑1970999 | tGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCAt < 1:3716046/70‑1 (MQ=255)cgccacaATTTATTGTGACGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATc > 1:3671718/4‑71 (MQ=255) ccacaATTTATTGTGACGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCt > 1:200418/2‑70 (MQ=255) acaATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc > 1:1947603/1‑70 (MQ=255) acaATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc > 1:3052194/1‑70 (MQ=255) tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGa > 1:2152782/1‑70 (MQ=255) tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGAt < 1:2589048/71‑1 (MQ=255) tAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGAt < 1:3031234/71‑1 (MQ=255) ttGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGATGAGt < 1:3711332/71‑1 (MQ=39) ttGTGACGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATaa < 1:425405/69‑1 (MQ=255) tgtgGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGta > 1:1799519/1‑71 (MQ=255) gCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGATGAGtaagt < 1:106720/70‑4 (MQ=37) gCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGATGAGtaagt < 1:2106553/70‑4 (MQ=37) gCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAAc < 1:124423/71‑1 (MQ=255) cgtaTTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAAcc < 1:3028940/68‑1 (MQ=255) cGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTc < 1:2995851/50‑1 (MQ=255) cGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTc < 1:1940383/50‑1 (MQ=255) cGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAAcc < 1:3024710/71‑1 (MQ=255) cGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAAcc < 1:1237338/71‑1 (MQ=255) ggATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCg < 1:1411514/71‑1 (MQ=255) gATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCg > 1:2502941/1‑70 (MQ=255) gATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCg > 1:1134602/1‑70 (MQ=255) cGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCt > 1:195207/1‑70 (MQ=255) cGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTg > 1:1680106/1‑71 (MQ=255) cGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTg > 1:427020/1‑71 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCa < 1:2713515/39‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCa < 1:3221184/39‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCa < 1:2717827/39‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCa < 1:3256440/39‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCa < 1:3509468/39‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCa < 1:3202868/39‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCa < 1:3600916/39‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCa < 1:1617674/39‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCa < 1:1666465/39‑1 (MQ=255) gCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTc < 1:247472/44‑1 (MQ=255) ctgaaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGcc > 1:2993152/4‑71 (MQ=255) cTAAATTAAGCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGAt > 1:2180128/1‑48 (MQ=38) cTAAATTAAGCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGAt > 1:932372/1‑48 (MQ=38) cTAAATTAAGCGGATTCAAGTAACACAGGACTTACTTATCTTGAAGAt > 1:2475598/1‑48 (MQ=38) cTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGcc > 1:977673/1‑71 (MQ=255) cTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGcc > 1:1932885/1‑71 (MQ=255) cTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGcc > 1:2080262/1‑71 (MQ=255) tAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAg < 1:1605696/50‑1 (MQ=255) aaaTTAAGCGGATTCAAGTAACACAGGACTTACTc > 1:1970180/1‑35 (MQ=255) aaaTTAAGCGGATTCAAGTAACACAGGACTTACTCa > 1:1958656/1‑36 (MQ=255) aaaTTAAGCGGATTCAAGTAACACAGGACTTACTCa > 1:3105445/1‑36 (MQ=255) aaaTTAAGCGGATTCAAGTAACACAGGACTTACTCa > 1:3012636/1‑36 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCt < 1:2813879/69‑1 (MQ=255) aaTTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCt < 1:3136359/69‑1 (MQ=255) tAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAc > 1:179689/1‑71 (MQ=255) aaGCGGATTCAAGTAACACAAGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAAc < 1:3215923/70‑1 (MQ=255) cGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCtt > 1:836573/1‑71 (MQ=255) ggATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc < 1:3374487/71‑1 (MQ=255) ggATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc < 1:3426534/71‑1 (MQ=255) ggATTCAAGTAACACAGGACTTACTCATCTACAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTc < 1:3131050/71‑1 (MQ=255) cAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCt > 1:126548/1‑52 (MQ=255) aaGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTTGAActc < 1:480183/62‑1 (MQ=255) aaGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActc < 1:258844/62‑1 (MQ=255) aGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAActc < 1:2566275/61‑1 (MQ=255) aGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAAc < 1:1924486/71‑1 (MQ=255) gTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACt > 1:835745/1‑71 (MQ=255) acacAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgtt < 1:1073160/71‑1 (MQ=255) acacAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgtt > 1:63040/1‑71 (MQ=255) cacaGGACTTACTCATCTTCAAGATAAGTATAACCGTACa < 1:2051402/40‑1 (MQ=255) cacaGGACTTACTCATCTTCAAGATAAGTATAACCGTACa < 1:2024216/40‑1 (MQ=255) cacaGGACTTACTCATCTTCAAGATAAGTATAACCGTACa < 1:1393105/40‑1 (MQ=255) acaGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGaa < 1:1292070/52‑1 (MQ=255) acaGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgttgt < 1:2133196/71‑1 (MQ=255) acaGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgttgt < 1:1482143/71‑1 (MQ=255) acaGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACtgttgt < 1:2409955/71‑1 (MQ=255) caGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTgttgtt < 1:411744/71‑1 (MQ=255) aGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTgttgtt > 1:2504112/1‑70 (MQ=255) ggACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGttgttg > 1:62342/1‑70 (MQ=255) aCTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCtt < 1:828026/52‑1 (MQ=255) aCTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCtt < 1:872425/52‑1 (MQ=255) aCTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAg < 1:2913609/71‑1 (MQ=255) cTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGt > 1:151927/1‑71 (MQ=255) aCTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGtt < 1:1456861/69‑1 (MQ=255) | TGTCACAATAAATTGTGGCGGATTATCACCGCTAAATTAAGCGGATTCAAGTAACACAGGACTTACTCATCTTCAAGATAAGTATAACCGTACAAACCTGCCTCGAACTCTTCAAGGAACTGTTGTTGCAGTT > Exported/1970867‑1970999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |