| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,976,997 | G→A | 6.7% | L83L (TTG→TTA) | sprT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,976,997 | 0 | G | A | 6.7% | 241.0 / 5.5 | 75 | L83L (TTG→TTA) | sprT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (40/30); new base A (2/3); total (42/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
TCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGC > Exported/1976928‑1977067 | tCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGc > 1:341530/1‑71 (MQ=255) gCTGTTGGAAAACAGTGAAGCTTTTATTTAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATgg > 1:192099/1‑70 (MQ=255) cTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGa < 1:823497/70‑1 (MQ=255) cTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGa < 1:793841/70‑1 (MQ=255) tGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGaaa > 1:3529479/1‑71 (MQ=255) gTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGaaa > 1:628347/1‑70 (MQ=255) ttGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGt < 1:2402843/62‑1 (MQ=255) ggAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAtt > 1:1752393/1‑71 (MQ=255) ggAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAt < 1:961574/70‑1 (MQ=255) aaaaCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAtt < 1:3114092/69‑1 (MQ=255) aaCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGc > 1:2069925/1‑52 (MQ=255) aaCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGc > 1:1761578/1‑52 (MQ=255) aaCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGc > 1:530829/1‑52 (MQ=255) aaCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGc > 1:3550052/1‑52 (MQ=255) aaCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAAc > 1:2204580/1‑64 (MQ=255) aaCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCgg > 1:696517/1‑71 (MQ=255) aaCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCgg > 1:86155/1‑71 (MQ=255) aaCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCgg > 1:1183451/1‑71 (MQ=255) aCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGc > 1:2082764/1‑71 (MQ=255) aCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGc > 1:2705401/1‑71 (MQ=255) tGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCcgcg > 1:3550337/1‑71 (MQ=255) tGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACAGTTGCTGGTATGGAAACATTTCGGCcgcg > 1:2378894/1‑71 (MQ=255) aaGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCcgcg > 1:889215/1‑69 (MQ=255) aaGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTACTGGTATGGAAACATTTCGGCCGCGt < 1:2741821/70‑1 (MQ=255) aGCTTTTATTGTAGAAGTGGTACCGCACGAACTGGCTCATTTACTGGTAt < 1:1520907/50‑1 (MQ=255) gCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCcgc > 1:2048602/1‑66 (MQ=255) gCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgc < 1:1211778/71‑1 (MQ=255) cTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTgg < 1:453098/45‑1 (MQ=255) cTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTgg < 1:3306780/45‑1 (MQ=255) cTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgcg < 1:2797147/71‑1 (MQ=255) ttttATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgcgc > 1:830225/1‑71 (MQ=255) ttGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCcaca < 1:791382/70‑1 (MQ=255) tGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACAt < 1:493973/70‑1 (MQ=255) agaagTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGc > 1:1285206/1‑70 (MQ=255) agaagTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGc > 1:1044504/1‑70 (MQ=255) agaagTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGc > 1:3388384/1‑70 (MQ=255) agaagTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGc > 1:132761/1‑70 (MQ=255) agaagTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGc > 1:1527548/1‑70 (MQ=255) gaagTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCaa < 1:2927198/71‑1 (MQ=255) aagTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCaaa < 1:451684/71‑1 (MQ=255) gTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGt < 1:1013744/53‑1 (MQ=255) gTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGt < 1:996270/53‑1 (MQ=255) gTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAaga < 1:2579457/71‑1 (MQ=255) gTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAaga < 1:1509750/71‑1 (MQ=255) tGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgc < 1:1928924/55‑1 (MQ=255) gTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtg > 1:1722643/1‑71 (MQ=255) gTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagt < 1:2905113/70‑1 (MQ=255) tACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtgg < 1:2710432/71‑1 (MQ=255) tACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtgg < 1:2303808/71‑1 (MQ=255) tACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtgg < 1:829249/71‑1 (MQ=255) tACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtg > 1:953264/1‑70 (MQ=255) ccGCACGAACTGGCACATTTACTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaa < 1:709858/71‑1 (MQ=255) cGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaag < 1:2868789/71‑1 (MQ=255) cGCACGAACTGGCACATTTACTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaag > 1:1750774/1‑71 (MQ=255) gCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgcg > 1:1441273/1‑49 (MQ=255) gCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaagt > 1:270088/1‑71 (MQ=255) gCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaagt < 1:2135255/71‑1 (MQ=255) aCGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgcg > 1:2108471/1‑47 (MQ=255) aCGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaagtg > 1:99183/1‑70 (MQ=255) aCGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaagtg > 1:751459/1‑70 (MQ=255) cGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtg < 1:1433358/63‑1 (MQ=255) cGAACTGGCACATTTACTGGTATGGAAACATTTCg > 1:1420592/1‑35 (MQ=255) gAACTGGCACATTTGCTGGTATGTAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtg < 1:1392595/62‑1 (MQ=255) gAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGgat > 1:1136555/1‑71 (MQ=255) aaCTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtggaa < 1:2499333/64‑1 (MQ=255) aaCTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGgat > 1:3139263/1‑70 (MQ=255) aCTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGgatga < 1:2248028/71‑1 (MQ=255) cacaTTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGa > 1:2567263/1‑70 (MQ=255) acaTTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGagtgg > 1:3619149/1‑55 (MQ=255) tttGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACg > 1:1441333/1‑71 (MQ=255) tttGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACg < 1:1952809/71‑1 (MQ=255) tttGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACg < 1:1351010/71‑1 (MQ=255) ttGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGt > 1:1288551/1‑71 (MQ=255) tGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTg > 1:547075/1‑71 (MQ=255) tGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTg > 1:92536/1‑71 (MQ=255) gCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGc > 1:1499473/1‑71 (MQ=255) | TCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCAAAGAGTGGAAGTGGATGATGGAAAACGTGC > Exported/1976928‑1977067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |