| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,012,045 | T→C | 56.3% | M63V (ATG→GTG) | yqhA ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,012,045 | 0 | T | C | 56.3% | 47.2 / 132.9 | 103 | M63V (ATG→GTG) | yqhA | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (29/16); new base C (34/24); total (63/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGT > Exported/2011977‑2012113 | cAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCacat > 1:474096/1‑71 (MQ=255) aGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATc > 1:1761622/1‑71 (MQ=255) gCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATcc < 1:3360304/71‑1 (MQ=255) gCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATc > 1:2305987/1‑70 (MQ=255) gtgAGACGAAATTCTCATAACCGGAAAACATCACCATCACGAGCAAACCGCCAACCAGCGTCacat > 1:1109528/1‑66 (MQ=255) gtgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCacat > 1:2687646/1‑66 (MQ=255) agaCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCTCCAACCAGCGTCACATCCACCAGc < 1:35122/71‑1 (MQ=255) aCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATccac > 1:2963635/1‑65 (MQ=255) cGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGTGTCATATCCACCAGCGa > 1:24499/1‑70 (MQ=255) cGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:714883/1‑70 (MQ=255) aaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:2644990/1‑67 (MQ=255) aaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAg > 1:3132352/1‑70 (MQ=255) aaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGc < 1:1487839/71‑1 (MQ=255) aaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGc < 1:2284995/71‑1 (MQ=255) aaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAg > 1:2632832/1‑70 (MQ=255) aaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAg > 1:3069429/1‑70 (MQ=255) aaTTCTCATAACCGGAAAACATCAACATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAg > 1:1632547/1‑70 (MQ=255) tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCaa > 1:338581/1‑70 (MQ=255) aTAACCTGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAacac > 1:3550920/1‑69 (MQ=255) aTAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAacac > 1:1546338/1‑69 (MQ=255) aTAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAaca < 1:2017603/68‑1 (MQ=255) aTAACCGGAAAACAACACCATCACCAGCAAACCGCCAACCAGCGTCACATCAACCAGCGACAGCAacac > 1:1047441/1‑69 (MQ=255) tAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAg > 1:1931848/1‑71 (MQ=255) ccGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATccacc < 1:892050/51‑1 (MQ=255) ccGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATccacc < 1:491871/51‑1 (MQ=255) ccGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATccacc < 1:869635/51‑1 (MQ=255) ccGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCa < 1:2117197/61‑1 (MQ=255) cGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGc < 1:2008297/53‑1 (MQ=255) cGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGc < 1:3736376/53‑1 (MQ=255) cGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATc < 1:255667/71‑1 (MQ=255) gAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCa > 1:2077479/1‑70 (MQ=255) aaaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCaaa > 1:2727326/1‑71 (MQ=255) aaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCa < 1:3129566/55‑1 (MQ=255) aaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCa < 1:431192/55‑1 (MQ=255) atcaccatcaccAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGa < 1:56889/71‑1 (MQ=255) tcaccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAaca < 1:1441746/54‑1 (MQ=255) tcaccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAt > 1:434896/1‑71 (MQ=255) tcaccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGAt > 1:901629/1‑71 (MQ=255) tcaccatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGAt < 1:175563/71‑1 (MQ=255) tcaccatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGAt > 1:3407050/1‑71 (MQ=255) tcaccatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGAt > 1:930451/1‑71 (MQ=255) tcaccatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGAt > 1:2704879/1‑71 (MQ=255) caccatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt > 1:279198/1‑71 (MQ=255) accatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAaca > 1:3536616/1‑52 (MQ=255) accatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTc < 1:3490914/71‑1 (MQ=255) accatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTc > 1:572757/1‑71 (MQ=255) accatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTc < 1:2578688/71‑1 (MQ=255) ccatcaccaGCAAACCGCCAAACAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTcc > 1:865926/1‑71 (MQ=255) tcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGa > 1:1495438/1‑40 (MQ=255) tcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGc > 1:2522039/1‑70 (MQ=255) tcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGcc < 1:2735687/71‑1 (MQ=255) caccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCg > 1:946690/1‑68 (MQ=255) caccaGCAAACCGCCAACCAGCGTCACATCCACCAGc > 1:72012/1‑37 (MQ=255) caccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACa > 1:2546129/1‑41 (MQ=255) caccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGAtt < 1:3669210/65‑1 (MQ=255) accaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCAt > 1:31416/1‑71 (MQ=255) ccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc > 1:3172179/1‑71 (MQ=255) caGCAAACCGCCACCCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc > 1:3147865/1‑70 (MQ=255) caGCAAACCGCCAACCAGTGTCATATCCACCAGCGACAGCAACACGAGGATCaa < 1:3639822/54‑1 (MQ=255) caGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCaa < 1:2740972/42‑1 (MQ=255) caGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCaa < 1:1817445/42‑1 (MQ=255) caGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCaa < 1:2078523/42‑1 (MQ=255) caGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCaa < 1:2241317/42‑1 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGTTCAAATCTGATTCCGCCATc > 1:2924981/1‑69 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTc > 1:1976657/1‑62 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTc > 1:3327127/1‑62 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTc > 1:1890048/1‑62 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTc > 1:768264/1‑62 (MQ=255) aGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTc > 1:271086/1‑62 (MQ=255) aGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc > 1:3569197/1‑69 (MQ=255) aGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCga < 1:1045887/71‑1 (MQ=255) aGCAAACCGCCAACCAGCGACATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCga < 1:1172324/71‑1 (MQ=255) gCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAaca > 1:1169567/1‑42 (MQ=255) gCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt > 1:2156609/1‑54 (MQ=255) gCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc > 1:2995938/1‑68 (MQ=255) gCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc > 1:2797760/1‑68 (MQ=255) gCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc > 1:647921/1‑68 (MQ=255) gCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag < 1:880488/71‑1 (MQ=255) gCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag < 1:1310043/71‑1 (MQ=255) cAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag > 1:3170839/1‑70 (MQ=255) aaaCCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgaga < 1:1716702/70‑1 (MQ=255) aaaCCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCg > 1:3015649/1‑67 (MQ=255) aCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag > 1:1751704/1‑67 (MQ=255) ccGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCCAGGAGat > 1:3010257/1‑71 (MQ=255) cGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata < 1:2877262/71‑1 (MQ=255) cGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGat > 1:593873/1‑70 (MQ=255) ccAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt > 1:1933245/1‑46 (MQ=255) ccAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATAtt > 1:3504524/1‑71 (MQ=255) cAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATAGAGAAGATATTc < 1:950078/71‑1 (MQ=255) ccAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGttcttccag > 1:1020770/1‑68 (MQ=255) ccAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCgg > 1:2467866/1‑70 (MQ=255) gCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAg > 1:2334965/1‑70 (MQ=255) gCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaa > 1:1139147/1‑58 (MQ=255) gCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAg > 1:138587/1‑70 (MQ=255) gCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGt < 1:696375/71‑1 (MQ=255) gCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGt < 1:2583950/71‑1 (MQ=255) cGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGt < 1:538505/70‑1 (MQ=255) cGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTa > 1:449280/1‑71 (MQ=255) gTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTAc > 1:624905/1‑71 (MQ=255) atatCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGt > 1:79624/1‑70 (MQ=255) | CAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGT > Exported/2011977‑2012113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |