Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,042,842 T→A 13.3% intergenic (‑190/‑79) glgS ← / → yqiJ predicted glycogen synthesis protein/predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,042,8420TA13.3% 119.8 / 10.5 45intergenic (‑190/‑79)glgS/yqiJpredicted glycogen synthesis protein/predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (19/20);  new base A (3/3);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

ACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAT  >  Exported/2042773‑2042909
                                                                     |                                                                   
acTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttatt                                                                    <  1:2137477/71‑1 (MQ=255)
  tttttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttaataa                                                                  >  1:1339878/1‑70 (MQ=255)
  tttttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttaataa                                                                  >  1:2195738/1‑70 (MQ=255)
    tttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttattatt                                                                 <  1:3680133/70‑1 (MQ=255)
     ttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                               >  1:378569/1‑71 (MQ=255)
     ttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                               >  1:2219502/1‑71 (MQ=255)
     ttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                               >  1:159412/1‑71 (MQ=255)
        gTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                               >  1:2905675/1‑68 (MQ=255)
           aaaaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAaca                                                             >  1:2128573/1‑67 (MQ=255)
           aaaaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAaca                                                             >  1:3594471/1‑67 (MQ=255)
             aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATa                                                       <  1:3417513/71‑1 (MQ=255)
                tAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATaaa                                                     >  1:374216/1‑70 (MQ=255)
                  aaaaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATg                                                   <  1:1044298/70‑1 (MQ=255)
                   aaaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTa                                                 <  1:807150/71‑1 (MQ=255)
                    aaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTaa                                                <  1:3104638/71‑1 (MQ=255)
                    aaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTaa                                                <  1:1768872/71‑1 (MQ=255)
                       ccATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCa                                             <  1:877496/71‑1 (MQ=255)
                       ccATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCa                                             <  1:201624/71‑1 (MQ=255)
                        cATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGAt                                                        <  1:1640929/59‑1 (MQ=255)
                          tatTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttatta                                                                   >  1:516764/1‑46 (MQ=255)
                          tatTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAgg                                          <  1:3465889/71‑1 (MQ=255)
                           atTAAAAGATTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATg                                                   >  1:1482031/1‑61 (MQ=255)
                           atTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAgg                                          >  1:1117647/1‑70 (MQ=255)
                            ttAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGa                                         <  1:3370330/70‑1 (MQ=255)
                             tAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGaaa                                       >  1:1551535/1‑71 (MQ=255)
                                   aCTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTa                                 <  1:2843360/71‑1 (MQ=255)
                                       tctcTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACa                               >  1:3294860/1‑69 (MQ=255)
                                         tctTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                               >  1:2690107/1‑35 (MQ=255)
                                         tctTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                               >  1:1843199/1‑35 (MQ=255)
                                           ttCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGAt                                                        <  1:1643102/40‑1 (MQ=255)
                                           ttCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGt                           <  1:1413788/69‑1 (MQ=255)
                                           ttCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGt                           <  1:1618637/69‑1 (MQ=255)
                                             ccAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAg                                               >  1:540943/1‑47 (MQ=255)
                                               aGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAatat                     <  1:280158/71‑1 (MQ=255)
                                               aGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAatat                     <  1:429296/71‑1 (MQ=255)
                                                  aCTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGc                  <  1:3688374/71‑1 (MQ=255)
                                                       tctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGc                  <  1:1184487/66‑1 (MQ=255)
                                                         tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGt                >  1:2797279/1‑66 (MQ=255)
                                                         tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACt            >  1:3545435/1‑70 (MQ=255)
                                                         tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACt            >  1:662451/1‑70 (MQ=255)
                                                          aaCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTa           >  1:1093924/1‑70 (MQ=255)
                                                           aCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTaaa         >  1:829079/1‑71 (MQ=255)
                                                           aCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTaa          <  1:2417443/70‑1 (MQ=255)
                                                           aCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTaa          <  1:2529947/70‑1 (MQ=255)
                                                               gtgtTAATAATAACACAGGTAAATGTAAGCAAGGa                                         <  1:749202/35‑1 (MQ=17)
                                                               gtgtTAATAATAACACAGGTAAATGTAAGCAAGGAAACTATTACAAAGTaaa                        <  1:2270071/52‑1 (MQ=37)
                                                                tgtTAATAATAACACAGGTAAATGTAAGCAAGGAAACTATTACAAAGTaaa                        <  1:2635767/51‑1 (MQ=37)
                                                                 gtTAATAATAACACAGGTAAATGTAAGCAAGGaaa                                       >  1:3644511/1‑35 (MQ=18)
                                                                 gtTAATAATAACACAGGTAAATGTAAGCAAGGaaa                                       >  1:1419580/1‑35 (MQ=18)
                                                                 gtTAATAATAACACAGGTAAATGTAAGCAAGGaaa                                       >  1:661771/1‑35 (MQ=18)
                                                                  ttattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAt  <  1:2041158/71‑1 (MQ=255)
                                                                  ttattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAt  >  1:3708411/1‑71 (MQ=255)
                                                                  ttattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAt  <  1:1392834/71‑1 (MQ=255)
                                                                     |                                                                   
ACTTTTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAT  >  Exported/2042773‑2042909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: