Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,042,845 T→A 13.3% intergenic (‑193/‑76) glgS ← / → yqiJ predicted glycogen synthesis protein/predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,042,8450TA13.3% 119.1 / 9.2 45intergenic (‑193/‑76)glgS/yqiJpredicted glycogen synthesis protein/predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (19/20);  new base A (3/3);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGA  >  Exported/2042777‑2042913
                                                                    |                                                                    
tttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGttattatt                                                                     <  1:3680133/70‑1 (MQ=255)
 ttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                                   >  1:159412/1‑71 (MQ=255)
 ttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                                   >  1:378569/1‑71 (MQ=255)
 ttAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                                   >  1:2219502/1‑71 (MQ=255)
    gTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                                   >  1:2905675/1‑68 (MQ=255)
       aaaaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAaca                                                                 >  1:3594471/1‑67 (MQ=255)
       aaaaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAaca                                                                 >  1:2128573/1‑67 (MQ=255)
         aaaTAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATa                                                           <  1:3417513/71‑1 (MQ=255)
            tAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATaaa                                                         >  1:374216/1‑70 (MQ=255)
              aaaaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATg                                                       <  1:1044298/70‑1 (MQ=255)
               aaaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTa                                                     <  1:807150/71‑1 (MQ=255)
                aaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTaa                                                    <  1:3104638/71‑1 (MQ=255)
                aaaCCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTaa                                                    <  1:1768872/71‑1 (MQ=255)
                   ccATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCa                                                 <  1:877496/71‑1 (MQ=255)
                   ccATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCa                                                 <  1:201624/71‑1 (MQ=255)
                    cATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGAt                                                            <  1:1640929/59‑1 (MQ=255)
                      tatTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAgg                                              <  1:3465889/71‑1 (MQ=255)
                       atTAAAAGATTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATg                                                       >  1:1482031/1‑61 (MQ=255)
                       atTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAgg                                              >  1:1117647/1‑70 (MQ=255)
                        ttAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGa                                             <  1:3370330/70‑1 (MQ=255)
                         tAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGaaa                                           >  1:1551535/1‑71 (MQ=255)
                               aCTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTa                                     <  1:2843360/71‑1 (MQ=255)
                                   tctcTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACa                                   >  1:3294860/1‑69 (MQ=255)
                                     tctTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                                   >  1:2690107/1‑35 (MQ=255)
                                     tctTCCAGAACTTCTCTAACCTGTGTTATTATTaa                                                                   >  1:1843199/1‑35 (MQ=255)
                                       ttCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGAt                                                            <  1:1643102/40‑1 (MQ=255)
                                       ttCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGt                               <  1:1413788/69‑1 (MQ=255)
                                       ttCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGt                               <  1:1618637/69‑1 (MQ=255)
                                         ccAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAg                                                   >  1:540943/1‑47 (MQ=255)
                                           aGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAatat                         <  1:280158/71‑1 (MQ=255)
                                           aGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAatat                         <  1:429296/71‑1 (MQ=255)
                                              aCTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGc                      <  1:3688374/71‑1 (MQ=255)
                                                   tctAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGc                      <  1:1184487/66‑1 (MQ=255)
                                                     tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGt                    >  1:2797279/1‑66 (MQ=255)
                                                     tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACt                >  1:3545435/1‑70 (MQ=255)
                                                     tAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACt                >  1:662451/1‑70 (MQ=255)
                                                      aaCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTa               >  1:1093924/1‑70 (MQ=255)
                                                       aCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTaaa             >  1:829079/1‑71 (MQ=255)
                                                       aCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTaa              <  1:2529947/70‑1 (MQ=255)
                                                       aCCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTaa              <  1:2417443/70‑1 (MQ=255)
                                                           gtgtTAATAATAACACAGGTAAATGTAAGCAAGGa                                             <  1:749202/35‑1 (MQ=17)
                                                           gtgtTAATAATAACACAGGTAAATGTAAGCAAGGAAACTATTACAAAGTaaa                            <  1:2270071/52‑1 (MQ=37)
                                                            tgtTAATAATAACACAGGTAAATGTAAGCAAGGAAACTATTACAAAGTaaa                            <  1:2635767/51‑1 (MQ=37)
                                                             gtTAATAATAACACAGGTAAATGTAAGCAAGGaaa                                           >  1:3644511/1‑35 (MQ=18)
                                                             gtTAATAATAACACAGGTAAATGTAAGCAAGGaaa                                           >  1:1419580/1‑35 (MQ=18)
                                                             gtTAATAATAACACAGGTAAATGTAAGCAAGGaaa                                           >  1:661771/1‑35 (MQ=18)
                                                              ttattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAt      >  1:3708411/1‑71 (MQ=255)
                                                              ttattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAt      <  1:2041158/71‑1 (MQ=255)
                                                              ttattattaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACAt      <  1:1392834/71‑1 (MQ=255)
                                                                   attaACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGa  <  1:2304077/70‑1 (MQ=255)
                                                                    |                                                                    
TTTAGTGAAAAATAAAAAACCATATTAAAAGACTTTCTCTTCCAGAACTTCTCTAACCTGTGTTATTATTAACACAGATAAATGTAAGCAAGGAAACTATTACAAAGTAAATATTGCGTCACTAAATGGACATTGGA  >  Exported/2042777‑2042913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: