Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,044,054 T→C 11.3% F160L (TTT→CTT)  yqiK → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,044,0540TC11.3% 192.0 / 14.6 71F160L (TTT→CTT) yqiKconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/38);  new base C (4/4);  total (29/42)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

ATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTT  >  Exported/2043984‑2044123
                                                                      |                                                                     
aTTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACt                                                                       <  1:1617747/71‑1 (MQ=255)
  ttGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACttt                                                                     >  1:2579939/1‑71 (MQ=255)
  ttGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACttt                                                                     >  1:1581517/1‑71 (MQ=255)
   tGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGGACttt                                                                     <  1:1329441/70‑1 (MQ=255)
   tGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACttt                                                                     <  1:1867160/70‑1 (MQ=255)
   tGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACCTtg                                                                    >  1:1006117/1‑71 (MQ=255)
    gTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACttt                                                                     <  1:1696046/69‑1 (MQ=255)
      cGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTtgtg                                                                  <  1:890959/70‑1 (MQ=255)
       gATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGc                                                                 <  1:261097/70‑1 (MQ=255)
       gATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGc                                                                 <  1:282074/70‑1 (MQ=255)
         tGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCCCGAGAACTTTGTGCAgg                                                              <  1:1675906/71‑1 (MQ=255)
           cccTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACCTTGTGCAggg                                                             <  1:1366267/70‑1 (MQ=255)
            ccTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAggggg                                                           <  1:2199432/71‑1 (MQ=255)
            ccTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAggggg                                                           <  1:95923/71‑1 (MQ=255)
             cTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGt                                                          >  1:3378091/1‑71 (MQ=255)
              tCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACtt                                                                      <  1:2089792/58‑1 (MQ=255)
               ccGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTg                                                         >  1:1318030/1‑70 (MQ=255)
               ccGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTg                                                         >  1:1683386/1‑70 (MQ=255)
                cGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGc                                                        <  1:1907937/70‑1 (MQ=255)
                 gTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCaa                                                      <  1:1316489/71‑1 (MQ=255)
                    cAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCaaaa                                                    <  1:2975809/70‑1 (MQ=255)
                    cAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCaaaa                                                    <  1:1591926/70‑1 (MQ=255)
                    cAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCaaaa                                                    >  1:249216/1‑70 (MQ=255)
                    cAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAAt                                                   <  1:1493090/71‑1 (MQ=255)
                     aaCAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTtgt                                                                   >  1:3162958/1‑54 (MQ=255)
                     aaCAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATa                                                  <  1:1198806/71‑1 (MQ=255)
                     aaCAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATa                                                  <  1:2399282/71‑1 (MQ=255)
                     aaCAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACCTTGTGCAGGGGGTGCAAAATa                                                  <  1:609665/71‑1 (MQ=255)
                       cAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACCTTGTGCAGGGGGTGCAAAATACa                                                <  1:2980909/71‑1 (MQ=255)
                        aGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACa                                                <  1:2729998/70‑1 (MQ=255)
                        aGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAg                                               >  1:1485813/1‑71 (MQ=255)
                            gcgcAAATGACCATGCATGAGTTACAGGATACCCGTGAGAACTTTGTGCAGGGGGTGCAAAATACAGTgg                                            >  1:397038/1‑70 (MQ=255)
                            gcgcAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGAGCAGGGGGTGCAAAATACAGTgg                                            >  1:925993/1‑70 (MQ=255)
                              gcAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCag                                         <  1:1054671/71‑1 (MQ=255)
                                aaaTGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCag                                         >  1:3455314/1‑69 (MQ=255)
                                aaaTGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCa                                          >  1:2544417/1‑68 (MQ=255)
                                   tGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGAc                                    <  1:721248/71‑1 (MQ=255)
                                     aCCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt                                  <  1:248349/71‑1 (MQ=255)
                                     aCCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt                                  <  1:712057/71‑1 (MQ=255)
                                     aCCATGCATGAGTTACAGGATACCCGCGAGAACCTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt                                  >  1:531811/1‑71 (MQ=255)
                                      ccATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt                                  <  1:517182/70‑1 (MQ=255)
                                      ccATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTg                                 <  1:674810/71‑1 (MQ=255)
                                       catgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt                                  <  1:3083227/69‑1 (MQ=255)
                                         tgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt                                  >  1:247636/1‑67 (MQ=255)
                                         tgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt                                  >  1:1539664/1‑67 (MQ=255)
                                         tgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGt                                >  1:277451/1‑69 (MQ=255)
                                         tgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTc                               >  1:2750436/1‑70 (MQ=255)
                                         tgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTc                               >  1:3219855/1‑70 (MQ=255)
                                          gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTg                                 <  1:3729938/67‑1 (MQ=255)
                                          gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTg                                 <  1:2079627/67‑1 (MQ=255)
                                          gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCg                              >  1:2816739/1‑70 (MQ=255)
                                          gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCg                              >  1:2849381/1‑70 (MQ=255)
                                           catgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGa                             >  1:502125/1‑70 (MQ=255)
                                           catgAGTTACAGGATACCCGCGAGAACCTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGa                             <  1:1626122/70‑1 (MQ=255)
                                            atgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGTGTGCAAAATACAGTGGCAGAAGACCTGTCGa                             <  1:1686787/69‑1 (MQ=255)
                                             tgAGTTACAGGATACACGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGaaaa                          <  1:2358479/71‑1 (MQ=255)
                                               aGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAAc                        >  1:2666297/1‑71 (MQ=255)
                                                gTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCg                              <  1:661398/64‑1 (MQ=255)
                                                gTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACg                       <  1:3012710/71‑1 (MQ=255)
                                                gTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACg                       <  1:254175/71‑1 (MQ=255)
                                                   aCAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCagaa                                       >  1:1495644/1‑52 (MQ=255)
                                                   aCAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTc                    >  1:1683095/1‑71 (MQ=255)
                                                    cAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTc                    <  1:1382028/70‑1 (MQ=255)
                                                    cAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTc                    <  1:500912/70‑1 (MQ=255)
                                                    cAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCt                   >  1:962559/1‑71 (MQ=255)
                                                    cAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCt                   >  1:347175/1‑71 (MQ=255)
                                                         tACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGt                                >  1:3545185/1‑53 (MQ=255)
                                                         tACCCGCGAGAACCTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAAc              >  1:787809/1‑71 (MQ=255)
                                                           cccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACt             <  1:693731/70‑1 (MQ=255)
                                                           cccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTg            <  1:1365715/71‑1 (MQ=255)
                                                           cccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTg            <  1:1347495/71‑1 (MQ=255)
                                                           cccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTg            <  1:1321089/71‑1 (MQ=255)
                                                            ccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGaa               <  1:276465/67‑1 (MQ=255)
                                                              gcgAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACt             >  1:1981511/1‑67 (MQ=255)
                                                                   aaCCTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGt    >  1:1223944/1‑71 (MQ=255)
                                                                     cTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGttt  <  1:3602203/71‑1 (MQ=255)
                                                                     cTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGttt  <  1:2548385/71‑1 (MQ=255)
                                                                     cTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGttt  <  1:2413990/71‑1 (MQ=255)
                                                                      tttGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGga          <  1:639701/62‑1 (MQ=255)
                                                                      tttGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGga          <  1:1461890/62‑1 (MQ=255)
                                                                      |                                                                     
ATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTT  >  Exported/2043984‑2044123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: