Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 2,044,054 | T→C | 11.3% | F160L (TTT→CTT) | yqiK → | conserved hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 2,044,054 | 0 | T | C | 11.3% | 192.0 / 14.6 | 71 | F160L (TTT→CTT) | yqiK | conserved hypothetical protein |
Reads supporting (aligned to +/- strand): ref base T (25/38); new base C (4/4); total (29/42) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 |
ATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTT > Exported/2043984‑2044123 | aTTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACt < 1:1617747/71‑1 (MQ=255) ttGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACttt > 1:2579939/1‑71 (MQ=255) ttGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACttt > 1:1581517/1‑71 (MQ=255) tGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGGACttt < 1:1329441/70‑1 (MQ=255) tGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACttt < 1:1867160/70‑1 (MQ=255) tGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACCTtg > 1:1006117/1‑71 (MQ=255) gTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACttt < 1:1696046/69‑1 (MQ=255) cGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTtgtg < 1:890959/70‑1 (MQ=255) gATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGc < 1:261097/70‑1 (MQ=255) gATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGc < 1:282074/70‑1 (MQ=255) tGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCCCGAGAACTTTGTGCAgg < 1:1675906/71‑1 (MQ=255) cccTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACCTTGTGCAggg < 1:1366267/70‑1 (MQ=255) ccTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAggggg < 1:2199432/71‑1 (MQ=255) ccTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAggggg < 1:95923/71‑1 (MQ=255) cTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGt > 1:3378091/1‑71 (MQ=255) tCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACtt < 1:2089792/58‑1 (MQ=255) ccGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTg > 1:1318030/1‑70 (MQ=255) ccGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTg > 1:1683386/1‑70 (MQ=255) cGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGc < 1:1907937/70‑1 (MQ=255) gTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCaa < 1:1316489/71‑1 (MQ=255) cAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCaaaa < 1:2975809/70‑1 (MQ=255) cAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCaaaa < 1:1591926/70‑1 (MQ=255) cAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCaaaa > 1:249216/1‑70 (MQ=255) cAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAAt < 1:1493090/71‑1 (MQ=255) aaCAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTtgt > 1:3162958/1‑54 (MQ=255) aaCAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATa < 1:1198806/71‑1 (MQ=255) aaCAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATa < 1:2399282/71‑1 (MQ=255) aaCAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACCTTGTGCAGGGGGTGCAAAATa < 1:609665/71‑1 (MQ=255) cAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACCTTGTGCAGGGGGTGCAAAATACa < 1:2980909/71‑1 (MQ=255) aGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACa < 1:2729998/70‑1 (MQ=255) aGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAg > 1:1485813/1‑71 (MQ=255) gcgcAAATGACCATGCATGAGTTACAGGATACCCGTGAGAACTTTGTGCAGGGGGTGCAAAATACAGTgg > 1:397038/1‑70 (MQ=255) gcgcAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGAGCAGGGGGTGCAAAATACAGTgg > 1:925993/1‑70 (MQ=255) gcAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCag < 1:1054671/71‑1 (MQ=255) aaaTGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCag > 1:3455314/1‑69 (MQ=255) aaaTGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCa > 1:2544417/1‑68 (MQ=255) tGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGAc < 1:721248/71‑1 (MQ=255) aCCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt < 1:248349/71‑1 (MQ=255) aCCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt < 1:712057/71‑1 (MQ=255) aCCATGCATGAGTTACAGGATACCCGCGAGAACCTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt > 1:531811/1‑71 (MQ=255) ccATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt < 1:517182/70‑1 (MQ=255) ccATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTg < 1:674810/71‑1 (MQ=255) catgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt < 1:3083227/69‑1 (MQ=255) tgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt > 1:247636/1‑67 (MQ=255) tgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCt > 1:1539664/1‑67 (MQ=255) tgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGt > 1:277451/1‑69 (MQ=255) tgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTc > 1:2750436/1‑70 (MQ=255) tgcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTc > 1:3219855/1‑70 (MQ=255) gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTg < 1:3729938/67‑1 (MQ=255) gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTg < 1:2079627/67‑1 (MQ=255) gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCg > 1:2816739/1‑70 (MQ=255) gcatgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCg > 1:2849381/1‑70 (MQ=255) catgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGa > 1:502125/1‑70 (MQ=255) catgAGTTACAGGATACCCGCGAGAACCTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGa < 1:1626122/70‑1 (MQ=255) atgAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGTGTGCAAAATACAGTGGCAGAAGACCTGTCGa < 1:1686787/69‑1 (MQ=255) tgAGTTACAGGATACACGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGaaaa < 1:2358479/71‑1 (MQ=255) aGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAAc > 1:2666297/1‑71 (MQ=255) gTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCg < 1:661398/64‑1 (MQ=255) gTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACg < 1:3012710/71‑1 (MQ=255) gTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACg < 1:254175/71‑1 (MQ=255) aCAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCagaa > 1:1495644/1‑52 (MQ=255) aCAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTc > 1:1683095/1‑71 (MQ=255) cAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTc < 1:1382028/70‑1 (MQ=255) cAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTc < 1:500912/70‑1 (MQ=255) cAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCt > 1:962559/1‑71 (MQ=255) cAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCt > 1:347175/1‑71 (MQ=255) tACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGt > 1:3545185/1‑53 (MQ=255) tACCCGCGAGAACCTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAAc > 1:787809/1‑71 (MQ=255) cccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACt < 1:693731/70‑1 (MQ=255) cccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTg < 1:1365715/71‑1 (MQ=255) cccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTg < 1:1347495/71‑1 (MQ=255) cccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTg < 1:1321089/71‑1 (MQ=255) ccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGaa < 1:276465/67‑1 (MQ=255) gcgAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACt > 1:1981511/1‑67 (MQ=255) aaCCTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGt > 1:1223944/1‑71 (MQ=255) cTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGttt < 1:3602203/71‑1 (MQ=255) cTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGttt < 1:2548385/71‑1 (MQ=255) cTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGttt < 1:2413990/71‑1 (MQ=255) tttGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGga < 1:639701/62‑1 (MQ=255) tttGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGga < 1:1461890/62‑1 (MQ=255) | ATTTGTCGATGCCCTCCGTGCAACAGCTGCGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTT > Exported/2043984‑2044123 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |