Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,136,852 G→T 5.2% intergenic (‑75/+74) rpsO ← / ← truB 30S ribosomal subunit protein S15/tRNA pseudouridine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,136,8520GT 5.2% 328.1 / 2.5 96intergenic (‑75/+74)rpsO/truB30S ribosomal subunit protein S15/tRNA pseudouridine synthase
Reads supporting (aligned to +/- strand):  ref base G (57/34);  new base T (3/2);  total (60/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 101 total aligned reads displayed.

CTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTA  >  Exported/2136785‑2136922
                                                                   |                                                                      
cTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAgtgt                                                                     >  1:1607663/1‑71 (MQ=255)
 tCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAgtgt                                                                     >  1:213391/1‑70 (MQ=255)
  ccAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGtt                                                                    >  1:1106301/1‑70 (MQ=255)
   cAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGtt                                                                    >  1:1403445/1‑69 (MQ=255)
   cAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGtt                                                                    <  1:2405003/69‑1 (MQ=255)
   cAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTa                                                                   >  1:310945/1‑70 (MQ=255)
    aaaGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGtt                                                                    <  1:3702071/68‑1 (MQ=255)
     aaGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAAc                                                                <  1:1924985/71‑1 (MQ=255)
        tatataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAt                                                             <  1:551907/71‑1 (MQ=255)
        tatataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAt                                                             <  1:3145547/71‑1 (MQ=255)
         atataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAtt                                                            >  1:571201/1‑71 (MQ=255)
          tataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAttt                                                           <  1:3568197/71‑1 (MQ=255)
          tataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAtt                                                            <  1:106420/70‑1 (MQ=255)
          tataGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAAtt                                                            <  1:940605/70‑1 (MQ=255)
             aGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAc                                                         >  1:1300620/1‑70 (MQ=255)
              gAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGTAAATTGTTTAACACTTTGcgt                                                       <  1:3008278/71‑2 (MQ=37)
              gAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGTAAATTGTTTAACACTTTGcgt                                                       <  1:2934059/71‑2 (MQ=37)
              gAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcgc                                                       <  1:1493192/71‑1 (MQ=255)
              gAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcg                                                        >  1:2141521/1‑70 (MQ=255)
              gAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcg                                                        <  1:846311/70‑1 (MQ=255)
                 tGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGAc                                                    >  1:3107607/1‑71 (MQ=255)
                   aaaGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcg                                                        >  1:548241/1‑65 (MQ=255)
                   aaaGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGt                                                  >  1:1814420/1‑71 (MQ=255)
                    aaGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGt                                                  >  1:1774086/1‑70 (MQ=255)
                    aaGGACGACGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAAGCGACGt                                                  >  1:1913191/1‑70 (MQ=255)
                     aGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa                                                <  1:1065076/71‑1 (MQ=255)
                      ggACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGtt                                                 >  1:1164704/1‑69 (MQ=255)
                       gACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcgc                                                       >  1:950517/1‑62 (MQ=255)
                       gACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcgc                                                       >  1:2866612/1‑62 (MQ=255)
                       gACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTAcgc                                                       >  1:657847/1‑62 (MQ=255)
                       gACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGAc                                                    <  1:3672877/65‑1 (MQ=255)
                         ctccGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACaa                                                              <  1:839327/51‑1 (MQ=255)
                              aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:734205/1‑70 (MQ=255)
                              aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:57251/1‑70 (MQ=255)
                              aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:3425046/1‑70 (MQ=255)
                              aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:700327/1‑70 (MQ=255)
                              aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:824382/1‑70 (MQ=255)
                              aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:1365212/1‑70 (MQ=255)
                              aTCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:1358636/1‑70 (MQ=255)
                                ctctAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:649637/1‑68 (MQ=255)
                                ctctAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:649635/1‑68 (MQ=255)
                                  ctAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGa                                                     >  1:112193/1‑53 (MQ=255)
                                  ctAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAACAATTTACGCGACGTTAAGCGGCa                                         <  1:3719325/64‑1 (MQ=255)
                                   tAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTAt                                     <  1:3480607/68‑1 (MQ=255)
                                    aaTTCAGCGATCCCAGTGTACGTTACGTAAATTGTTTAACACttt                                                           >  1:2084192/1‑45 (MQ=21)
                                    aaTTCAGCGATCCCAGTGTACGTTACGTAAATTGTTTAACACttt                                                           >  1:3032617/1‑45 (MQ=21)
                                    aaTTCAGAGATCCCAGTGTACGTTACGTAAATTGTTTAACACttt                                                           >  1:3027260/1‑45 (MQ=18)
                                     aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa                                                <  1:2498458/55‑1 (MQ=255)
                                     aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa                                                <  1:3653967/55‑1 (MQ=255)
                                     aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa                                                <  1:1744635/55‑1 (MQ=255)
                                     aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:1071269/1‑63 (MQ=255)
                                     aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        >  1:3484737/1‑63 (MQ=255)
                                     aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTa                                      >  1:2389565/1‑65 (MQ=255)
                                     aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTc                                   <  1:930057/68‑1 (MQ=255)
                                     aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCt                                  >  1:2824995/1‑69 (MQ=255)
                                     aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTa                                 >  1:1741347/1‑70 (MQ=255)
                                     aTTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTAc                                >  1:3707561/1‑71 (MQ=255)
                                        cAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTc                              <  1:1956606/70‑1 (MQ=255)
                                        cAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTc                              <  1:2104992/70‑1 (MQ=255)
                                         cgCGATACCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGa                                                     >  1:474675/2‑46 (MQ=255)
                                         aGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        <  1:1026229/59‑1 (MQ=255)
                                         aGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGt                            <  1:3603841/71‑1 (MQ=255)
                                          gCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTa                                      >  1:1230292/1‑60 (MQ=255)
                                          gCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTa                                      >  1:151853/1‑60 (MQ=255)
                                          gCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTa                                      >  1:1798180/1‑60 (MQ=255)
                                            gATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        <  1:223756/56‑1 (MQ=255)
                                             aTCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTa                                                <  1:335425/47‑1 (MQ=255)
                                               cccAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAg                                              >  1:1191493/1‑47 (MQ=255)
                                               cccAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAg                                              >  1:3623713/1‑47 (MQ=255)
                                               cccAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGAc                      >  1:1385670/1‑71 (MQ=255)
                                               ccaaGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGAc                      >  1:2125968/4‑71 (MQ=255)
                                                ccAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACTTTAAGc                                             <  1:482163/47‑1 (MQ=255)
                                                 cAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAg                                        <  1:760908/51‑1 (MQ=255)
                                                   gtgtACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGt                  >  1:3019671/1‑71 (MQ=255)
                                                   gtgtACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGt                  >  1:2189302/1‑71 (MQ=255)
                                                      tACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTAt               >  1:2943063/1‑71 (MQ=255)
                                                       aCGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATc              >  1:1952580/1‑71 (MQ=255)
                                                        cGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCg             >  1:2446745/1‑71 (MQ=255)
                                                         gTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCa                                         <  1:1095974/42‑1 (MQ=255)
                                                          ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGt                                       >  1:1724092/1‑43 (MQ=255)
                                                          ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGt                                       >  1:685018/1‑43 (MQ=255)
                                                          ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGt                                       >  1:598693/1‑43 (MQ=255)
                                                          ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGt                                       >  1:2409155/1‑43 (MQ=255)
                                                          ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGt                                       >  1:2885871/1‑43 (MQ=255)
                                                          ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGc            >  1:3712383/1‑70 (MQ=255)
                                                          ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCa           <  1:395454/71‑1 (MQ=255)
                                                          ttACGCAAAGTGTTAAACAATTTACGCGACGTTAAGAGGCAGt                                       >  1:2101742/1‑43 (MQ=255)
                                                           tACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTa                           >  1:3399040/1‑54 (MQ=255)
                                                            actcAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGc            >  1:3602217/4‑68 (MQ=255)
                                                             cGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCt                    >  1:2994281/1‑59 (MQ=255)
                                                             cGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCt                    >  1:242419/1‑59 (MQ=255)
                                                              gCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGAc       >  1:225774/1‑71 (MQ=255)
                                                              gCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGAc       <  1:388077/71‑1 (MQ=255)
                                                                aaaGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGa        <  1:2341763/68‑1 (MQ=255)
                                                                  aGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACg      >  1:2273553/1‑68 (MQ=255)
                                                                  aGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGtt   <  1:2375306/71‑1 (MQ=255)
                                                                  aGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGtt   <  1:1024666/71‑1 (MQ=255)
                                                                   gtgtTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCATGACGGTTa  <  1:1245749/71‑1 (MQ=255)
                                                                   gtgtTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTa  <  1:1276068/71‑1 (MQ=255)
                                                                   gtgtTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTa  <  1:1965784/71‑1 (MQ=255)
                                                                   |                                                                      
CTCCAAAGTATATAGAATGAAAGGACGCCGATCTCTAATTCAGCGATCCCAGTGTACGTTACGCAAAGTGTTAAACAATTTACGCGACGTTAAGCGGCAGTATTCTACTCGTAGCGACCTGTTATCGCAAGACGGTTA  >  Exported/2136785‑2136922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: