Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,197,407 C→T 8.4% intergenic (+15/‑75) degQ → / → degS serine endoprotease, periplasmic/serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,197,4070CT 8.4% 330.8 / 14.7 107intergenic (+15/‑75)degQ/degSserine endoprotease, periplasmic/serine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (51/47);  new base T (3/6);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGC  >  Exported/2197338‑2197474
                                                                     |                                                                   
catcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCa                                                                    >  1:837116/1‑71 (MQ=255)
catcGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCa                                                                    >  1:3633349/1‑71 (MQ=255)
   cGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCAt                                                                   >  1:336065/1‑69 (MQ=255)
    gCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAg                                                                <  1:2620565/71‑1 (MQ=255)
       cTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCt                                                             >  1:7704/1‑71 (MQ=255)
       cTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCt                                                             >  1:289366/1‑71 (MQ=255)
       cTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCt                                                             >  1:3173067/1‑71 (MQ=255)
       cTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCt                                                             <  1:1621938/71‑1 (MQ=255)
        tGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCt                                                             >  1:1136888/1‑70 (MQ=255)
        tGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCt                                                             >  1:3215858/1‑70 (MQ=255)
          cAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGTATCAGGCtt                                                            <  1:1275521/69‑1 (MQ=255)
            aaTTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACgt                                                        >  1:92083/1‑71 (MQ=255)
            aaTTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACgt                                                        <  1:821094/71‑1 (MQ=255)
              ttGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACgtgt                                                      <  1:63025/71‑1 (MQ=255)
              ttGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACgtgt                                                      <  1:2535274/71‑1 (MQ=255)
               tGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACgtgt                                                      >  1:1048099/1‑70 (MQ=255)
                   cgcgGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCtt                                                            >  1:3258488/1‑60 (MQ=255)
                   cgcgGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCtt                                                            >  1:3283010/1‑60 (MQ=255)
                   cgcgGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATg                                                  <  1:1512609/70‑1 (MQ=255)
                        cAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGt                                            >  1:235201/1‑71 (MQ=255)
                         aaTGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGtt                                           <  1:2617280/71‑1 (MQ=255)
                          aTGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGtt                                           >  1:1933988/1‑70 (MQ=255)
                           tGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTaa                                         <  1:1565171/71‑1 (MQ=255)
                           tGAAAGCATCTATCTGCTGATGCGTTAATGTCGAAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTaa                                         <  1:1713267/71‑1 (MQ=255)
                            gAAAGCATCTATCTGCTGATGCGTTAATGTCGTTAACCGGGCATCAGGCtt                                                            <  1:2882937/51‑1 (MQ=255)
                            gAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCtt                                                            <  1:932387/51‑1 (MQ=255)
                            gAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCtt                                                            <  1:128897/51‑1 (MQ=255)
                            gAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCtt                                                            <  1:2445196/51‑1 (MQ=255)
                            gAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAAc                                        >  1:3377319/1‑71 (MQ=255)
                              aaGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTc                                      >  1:3007376/1‑71 (MQ=255)
                              aaGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTc                                      >  1:269551/1‑71 (MQ=255)
                              aaGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTc                                      <  1:2671545/71‑1 (MQ=255)
                              aaGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTc                                      <  1:2528117/71‑1 (MQ=255)
                              aaGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTc                                      >  1:2484344/1‑71 (MQ=255)
                               aGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGTATCAGGCTTACgtgt                                                      <  1:2128855/54‑1 (MQ=255)
                               aGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGTATCAGGCTTACgtgt                                                      <  1:1996443/54‑1 (MQ=255)
                               aGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCg                                     >  1:856429/1‑71 (MQ=255)
                               aGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCg                                     >  1:3078013/1‑71 (MQ=255)
                               aGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCg                                     >  1:1385096/1‑71 (MQ=255)
                               aGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCg                                     <  1:3328486/71‑1 (MQ=255)
                               aGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCg                                     <  1:1282963/71‑1 (MQ=255)
                                gCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGTATCAGGCTTACgtgt                                                      <  1:47071/53‑1 (MQ=255)
                                gCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGTATCAGGCTTACgtgt                                                      <  1:609491/53‑1 (MQ=255)
                                gCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTAc                                                          <  1:3061494/49‑1 (MQ=255)
                                  atctatctGCTGATGCGTTAATGTCGTAAACCGGGCa                                                                    >  1:2636980/1‑37 (MQ=255)
                                  atctatctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTgg                                  <  1:3522822/71‑1 (MQ=255)
                                  atctatctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTgg                                  <  1:3509887/71‑1 (MQ=255)
                                  atctatctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTgg                                  <  1:2871512/71‑1 (MQ=255)
                                    ctatctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTa                                >  1:2811697/1‑71 (MQ=255)
                                    ctatctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTa                                >  1:1313360/1‑71 (MQ=255)
                                     tatctGCTGATGCGTTAATTTCGTAAACCGGGCATCAgg                                                               <  1:2791602/39‑1 (MQ=255)
                                     tatctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAt                               >  1:3675980/1‑71 (MQ=255)
                                     tatctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAt                               >  1:3570141/1‑71 (MQ=255)
                                     tatctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAt                               >  1:445764/1‑71 (MQ=255)
                                      atctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCg                                     <  1:3645432/64‑1 (MQ=255)
                                       tctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGt                                 <  1:462129/67‑1 (MQ=255)
                                       tctGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGt                                 <  1:3182018/67‑1 (MQ=255)
                                        ctgctgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgct                            >  1:3482058/1‑71 (MQ=255)
                                         tgctgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGa                                                    >  1:1970143/1‑46 (MQ=255)
                                         tgctgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGa                                                    >  1:1343771/1‑46 (MQ=255)
                                         tgctgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGa                                                    >  1:1610866/1‑46 (MQ=255)
                                            tgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgc                             <  1:108699/66‑1 (MQ=255)
                                            tgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgc                             <  1:810336/66‑1 (MQ=255)
                                            tgATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgc                             <  1:1923043/66‑1 (MQ=255)
                                              aTGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCgg                                             >  1:2999419/1‑48 (MQ=255)
                                              aTGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCgg                                             >  1:1119762/1‑48 (MQ=255)
                                              aTGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCgg                                             >  1:119094/1‑48 (MQ=255)
                                              aTGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCgg                                             >  1:2273142/1‑48 (MQ=255)
                                              aTGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCgg                                             >  1:3410820/1‑48 (MQ=255)
                                              aTGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCgg                                             >  1:2896789/1‑48 (MQ=255)
                                              aTGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctgctg                        <  1:3421278/69‑1 (MQ=255)
                                              aTGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctgctg                        <  1:1950008/69‑1 (MQ=255)
                                              aTGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGcc                      >  1:42321/1‑71 (MQ=255)
                                               tGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCg                     <  1:76542/71‑1 (MQ=255)
                                                   ttAATGTCGTAAACCGGGTATCAGGCTTACGtgtg                                                     <  1:2091493/35‑1 (MQ=255)
                                                   ttAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgct                            <  1:2155129/60‑1 (MQ=255)
                                                     aaTGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctgc                          >  1:2859880/1‑60 (MQ=255)
                                                     aaTGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgctgc                          >  1:1384359/1‑60 (MQ=255)
                                                     aaTGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTccc                <  1:1880511/70‑1 (MQ=255)
                                                     aaTGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCt               >  1:1525367/1‑71 (MQ=255)
                                                      aTGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCtt              <  1:739909/71‑1 (MQ=255)
                                                      aTGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCtt              >  1:430662/1‑71 (MQ=255)
                                                        gTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCtttt            >  1:2993937/1‑71 (MQ=255)
                                                         tCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCtttt            <  1:3121790/70‑1 (MQ=255)
                                                           gTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACt                                       >  1:2712442/1‑41 (MQ=255)
                                                           gTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTcc                 >  1:3529385/1‑63 (MQ=255)
                                                            tAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTaa        <  1:2986671/71‑1 (MQ=255)
                                                            tAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGCTCCCTTTTTTaa        <  1:2409413/71‑1 (MQ=255)
                                                              aaCCGGGTATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAAt       >  1:3334188/1‑70 (MQ=255)
                                                              aaCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAAt       >  1:1260633/1‑70 (MQ=255)
                                                              aaCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATg      <  1:857500/71‑1 (MQ=255)
                                                              aaCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATg      <  1:1489403/71‑1 (MQ=255)
                                                              aaCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATg      <  1:1025687/71‑1 (MQ=255)
                                                               aCCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGa     >  1:1327657/1‑71 (MQ=255)
                                                                ccGGGTATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGAc    >  1:2071816/1‑71 (MQ=255)
                                                                ccGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGAc    <  1:483979/71‑1 (MQ=255)
                                                                ccGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGAc    <  1:1975490/71‑1 (MQ=255)
                                                                 cGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACg   >  1:690795/1‑71 (MQ=255)
                                                                  gggCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGc  <  1:2999961/71‑1 (MQ=255)
                                                                   ggCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTAtgc                             <  1:37702/43‑1 (MQ=255)
                                                                     |                                                                   
CATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGATGCGTTAATGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGACGC  >  Exported/2197338‑2197474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: