| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,365,579 | T→C | 9.2% | K181K (AAA→AAG) | yigZ ← | predicted elongation factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,365,579 | 0 | T | C | 9.2% | 184.1 / 9.1 | 65 | K181K (AAA→AAG) | yigZ | predicted elongation factor |
| Reads supporting (aligned to +/- strand): ref base T (36/23); new base C (4/2); total (40/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
ATTCTTCAATCGCTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAATTTTGCCGTCA > Exported/2365509‑2365648 | attCTTCAATCGCTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACt > 1:3701765/1‑71 (MQ=255) ttcttcAATCGCTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACtt < 1:3021336/71‑1 (MQ=255) ttcttcAATCGCTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACt < 1:2029947/70‑1 (MQ=255) ttattcAATCGCTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACt < 1:1237187/67‑1 (MQ=255) cttcAATCGCTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACttt < 1:2650595/70‑1 (MQ=255) cttcAATCGCTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTc > 1:3737510/1‑71 (MQ=255) gCTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGccgccg < 1:469721/70‑1 (MQ=255) cTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACCTTCGCCGCCgg > 1:1953526/1‑70 (MQ=255) cTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACCTTCGCCGCCGGa < 1:36335/71‑1 (MQ=255) tAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCgg > 1:3055443/1‑69 (MQ=255) aaCAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAg > 1:2603754/1‑71 (MQ=255) aaCAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAg > 1:2513240/1‑71 (MQ=255) aCAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGaa < 1:650539/69‑1 (MQ=255) aCAATTGCAATGAACCACGGCTAAAATCCGACAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGaa < 1:1878840/69‑1 (MQ=255) cAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAgcg < 1:772017/71‑1 (MQ=255) aaTTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCgccgcc > 1:1712121/1‑63 (MQ=255) tGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCa < 1:1301611/70‑1 (MQ=255) tGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCa < 1:1208608/70‑1 (MQ=255) cAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAgc > 1:2246077/1‑64 (MQ=255) tGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCg > 1:2898508/1‑69 (MQ=255) tGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCg > 1:3478834/1‑69 (MQ=255) aaCCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTcgc > 1:3166247/1‑49 (MQ=255) aaCCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAgc > 1:1188638/1‑59 (MQ=255) aaCCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCgcag > 1:2531244/1‑70 (MQ=255) aCGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGaa > 1:133465/1‑71 (MQ=255) aCGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGaa > 1:2046607/1‑71 (MQ=255) aCGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGaa > 1:2485178/1‑71 (MQ=255) ggCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACg < 1:2830439/71‑1 (MQ=255) gCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGaa < 1:2357223/50‑1 (MQ=255) gCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACg > 1:145207/1‑70 (MQ=255) cTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGaa > 1:1303371/1‑71 (MQ=255) aaaaTCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATg > 1:75339/1‑71 (MQ=255) aaaaTCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATg > 1:2223926/1‑71 (MQ=255) aaaTCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGc > 1:668913/1‑71 (MQ=255) aTCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAGGCGCCACCCGCAGCAGAACGAATGCCt > 1:2913431/1‑71 (MQ=255) aTCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCt > 1:2187075/1‑71 (MQ=255) aTCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCt > 1:291110/1‑71 (MQ=255) aTCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCt > 1:1363936/1‑71 (MQ=255) aTCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCt > 1:3200119/1‑71 (MQ=255) aTCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCt > 1:3597497/1‑71 (MQ=255) ccGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACg < 1:2879080/62‑1 (MQ=255) cGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGAt < 1:2772910/71‑1 (MQ=255) cGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGAt < 1:2836908/71‑1 (MQ=255) ccAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATaa > 1:2370072/1‑71 (MQ=255) ccAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATaa < 1:1728118/71‑1 (MQ=255) cAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCGGAACGAATGCCTGATAAt < 1:1282192/71‑1 (MQ=255) ttGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTg > 1:1408299/1‑71 (MQ=255) tGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGt > 1:1934019/1‑71 (MQ=255) tGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGt > 1:1950760/1‑71 (MQ=255) tGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGt > 1:1495902/1‑71 (MQ=255) gCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCAc > 1:2400713/1‑38 (MQ=255) gAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGAt < 1:1670386/58‑1 (MQ=255) gAAAATTCAGCCACCTTCGCCGCCGGAAGGGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGAt < 1:2808753/71‑1 (MQ=255) aaaaTTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGtagcag < 1:159237/43‑1 (MQ=255) aaaaTTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGcagcag < 1:3727533/43‑1 (MQ=255) aaTTCAGCCACCTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAAt > 1:1013821/1‑71 (MQ=255) aaTTCAGCCACCTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAAt > 1:2247911/1‑71 (MQ=255) aaTTCAGCCACCTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAAt > 1:1245113/1‑71 (MQ=255) aTTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCAc < 1:1406791/60‑1 (MQ=255) aTTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAAtt < 1:360672/71‑1 (MQ=255) tCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAAtttt > 1:1481111/1‑71 (MQ=255) cAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAAt > 1:861355/1‑44 (MQ=255) aGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAATTTTGc < 1:1906826/71‑1 (MQ=255) gCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAATTTTGcc < 1:2883075/71‑1 (MQ=255) gCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAATTTTGcc < 1:1003062/71‑1 (MQ=255) gCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAATTTTGcc < 1:805595/71‑1 (MQ=255) ccACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAATTTTGCCg > 1:1462502/1‑71 (MQ=255) cACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAATTTTGCCGt < 1:3262026/71‑1 (MQ=255) aCTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATaa > 1:739200/1‑48 (MQ=255) aCTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAAtt > 1:1487412/1‑63 (MQ=255) tttCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAATTTTGCCGTca > 1:3380073/1‑70 (MQ=255) tttCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAATTTTGCCGTca > 1:2788493/1‑70 (MQ=255) | ATTCTTCAATCGCTAACAATTGCAATGAACCACGGCTAAAATCCGCCAGCTTTGCGGAAAATTCAGCCACTTTCGCCGCCGGAAGCGCCACCCGCAGCAGAACGAATGCCTGATAATCACTGTTGATAATTTTGCCGTCA > Exported/2365509‑2365648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |