| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,385,450 | C→T | 8.5% | L37L (CTG→CTA) | metE ← | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,385,450 | 0 | C | T | 8.5% | 179.4 / 6.1 | 59 | L37L (CTG→CTA) | metE | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (18/36); new base T (2/3); total (20/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01 | |||||||||||
GGCAGCAGGTCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAGCTCGCGACGCAGG > Exported/2385382‑2385519 | ggCAGCAGGTCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCcag > 1:3077301/1‑71 (MQ=255) gcagGTCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGt < 1:2571327/71‑1 (MQ=255) gcagGTCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGt > 1:1258883/1‑71 (MQ=255) gcagGTCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGt < 1:1621385/71‑1 (MQ=255) ggTCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGttct < 1:1708345/71‑1 (MQ=255) ggTCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGttct < 1:3615829/71‑1 (MQ=255) gTCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGttctt < 1:2961710/71‑1 (MQ=255) gTCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGt < 1:444203/67‑1 (MQ=255) tCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGttcttc < 1:195898/71‑1 (MQ=255) gATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGcc < 1:3485168/58‑1 (MQ=255) gATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGcc < 1:1387697/58‑1 (MQ=255) gATACCAGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGcc < 1:2972388/58‑1 (MQ=255) aTACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCAcg < 1:340748/71‑1 (MQ=255) ccGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTg < 1:434003/71‑1 (MQ=255) ccGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACACCCTACCGCCAGCAGTTCTTCACGCGTg < 1:3524741/71‑1 (MQ=255) cGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTgg < 1:2312362/71‑1 (MQ=255) cttgcttTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTg < 1:1341784/68‑1 (MQ=255) cttgcttTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAg < 1:2296140/71‑1 (MQ=255) cttgcttTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAg < 1:971099/71‑1 (MQ=255) cttgcttTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAg < 1:2742997/71‑1 (MQ=255) cttgcttTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAg > 1:3150684/1‑71 (MQ=255) cttgcttTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAg < 1:1295865/71‑1 (MQ=255) tgcttTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGttctt < 1:1128464/58‑1 (MQ=255) tttGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTcccc > 1:3377219/1‑71 (MQ=255) ttgATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGccc < 1:3360878/71‑1 (MQ=255) tgATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGttctt > 1:799547/1‑49 (MQ=255) tgATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGttctt > 1:115543/1‑49 (MQ=255) tgATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGttctt > 1:771016/1‑49 (MQ=255) tgATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGttctt > 1:2513374/1‑49 (MQ=255) cccAGTGACGAGCACGCAATTCACGCCCTACCGCcag > 1:1181718/1‑37 (MQ=255) cccAGTGACGAGCACGCAATTCACGCCCTACCGCcag > 1:71011/1‑37 (MQ=255) cccAGTGACGAGCACGCAATTCACGCCCTACCGCcag > 1:3667256/1‑37 (MQ=255) cccAGTGACGAGCACGCAATTCACGCCCTACCGCcag > 1:691520/1‑37 (MQ=255) cccAGTGACGAGCACGCAATTCACGCCCTACCGCca > 1:2750084/1‑36 (MQ=255) ccAGTGACGAGCACGCAATTCACGCCCTACCGCcagca > 1:51609/1‑38 (MQ=255) cAGTGACGAGCACGCAATTCACGCCCTACCGCTAGCAGTTCTTCACGCGTgg < 1:1136681/52‑1 (MQ=255) cAGTGACGAGCACGCAATTCACGCCCTACCGCTAGCAGTTCTTCACGCGTgg < 1:2789484/52‑1 (MQ=255) cAGTGACGAGCACGCAATTCACGCCCTACCGCTAGCAGTTCTTCACGCGTgg < 1:1073443/52‑1 (MQ=255) cAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACt < 1:1971335/71‑1 (MQ=255) aGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACt < 1:3433868/70‑1 (MQ=255) gTGACGAGCACGCAATTCACGCCCTACCGCTAGCAGTTCTTCACGCGTGGAg > 1:1501637/1‑52 (MQ=255) gTGACGAGCACGCAATTCACGCCCTACCGCTAGCAGTTCTTCACGCGTGGAg > 1:615484/1‑52 (MQ=255) tGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTc < 1:352904/71‑1 (MQ=255) tGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTc < 1:421107/71‑1 (MQ=255) aCGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCtt > 1:2639640/1‑71 (MQ=255) cGAGCACGCAATTCACGCCCTACCGCCAGCAGttctt < 1:2039108/37‑1 (MQ=255) cGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTg > 1:1628065/1‑71 (MQ=255) gAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTgc > 1:2356414/1‑71 (MQ=255) gCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCg > 1:2924249/1‑52 (MQ=255) aCGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCtt > 1:1039220/1‑65 (MQ=255) aCGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCtt < 1:1901171/71‑1 (MQ=255) gCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCtttt < 1:2544320/71‑1 (MQ=255) gCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCtttt < 1:1390840/71‑1 (MQ=255) gCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTAGAGTTCCCCGCCCAATAACTTTCTTGCGCtttt < 1:775952/71‑1 (MQ=255) aaTTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCtttttt > 1:954683/1‑71 (MQ=255) aaTTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCtttttt < 1:3600135/71‑1 (MQ=255) aaTTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCtttttt < 1:233038/71‑1 (MQ=255) ttCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCtttttt < 1:2707415/69‑1 (MQ=255) cACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAg < 1:1193991/70‑1 (MQ=255) aCGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAGCt < 1:634899/71‑1 (MQ=255) cGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAGCTc > 1:3374532/1‑71 (MQ=255) tcccTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCt < 1:2594863/58‑1 (MQ=255) gCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCt < 1:2291876/59‑1 (MQ=255) gCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCt < 1:2071600/59‑1 (MQ=255) ccTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAGCTcgc > 1:1017298/1‑70 (MQ=255) ccGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAGCTCGCGAc < 1:23429/69‑1 (MQ=255) ccGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAGCTCGCGACg > 1:2392070/1‑70 (MQ=255) ccGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAGCTCGCGACg > 1:3450511/1‑70 (MQ=255) gCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAGCTCGCGACGCAg > 1:998995/1‑71 (MQ=255) ccAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAGCTCGCGACGCAgg > 1:1019410/1‑71 (MQ=255) | GGCAGCAGGTCGATACCCGCTTGCTTTTGTTGATCCCAGTGACGAGCACGCAATTCACGCCCTACCGCCAGCAGTTCTTCACGCGTGGAGTTCCCCGCCCAATAACTTTCTTGCGCTTTTTTCAGCTCGCGACGCAGG > Exported/2385382‑2385519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |