Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,417,837 A→C 7.4% intergenic (‑111/+80) yifK ← / ← rffM predicted transporter/UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,417,8370AC 7.4% 132.7 / 6.2 54intergenic (‑111/+80)yifK/rffMpredicted transporter/UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
Reads supporting (aligned to +/- strand):  ref base A (23/27);  new base C (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

ATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAA  >  Exported/2417770‑2417906
                                                                   |                                                                     
aTCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaa                                                                     >  1:3355914/1‑70 (MQ=255)
aTCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaa                                                                     >  1:2634834/1‑70 (MQ=255)
 tCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaaa                                                                   >  1:2558217/1‑71 (MQ=255)
 tCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaa                                                                    <  1:1011010/70‑1 (MQ=255)
 tCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaa                                                                    <  1:3227538/70‑1 (MQ=255)
 tCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaa                                                                    <  1:3067028/70‑1 (MQ=255)
 tCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaa                                                                    <  1:2943275/70‑1 (MQ=255)
 tCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaa                                                                    <  1:2517039/70‑1 (MQ=255)
 tCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaa                                                                    <  1:561232/70‑1 (MQ=255)
  cGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAt                                                                  >  1:2950733/1‑71 (MQ=255)
   gTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAt                                                                  <  1:155786/70‑1 (MQ=255)
   gTTATGGGTACATCGACTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAt                                                                  <  1:2136598/70‑1 (MQ=255)
    ttATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                <  1:2870946/71‑1 (MQ=255)
    ttATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                <  1:2294767/71‑1 (MQ=255)
    ttATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                <  1:1829701/71‑1 (MQ=255)
      aTGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                >  1:3121177/1‑69 (MQ=255)
      aTGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                >  1:1216331/1‑69 (MQ=255)
      aTGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                >  1:1360315/1‑69 (MQ=255)
        gggTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                >  1:223658/1‑67 (MQ=255)
         ggTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAAt                                                            <  1:2877529/70‑1 (MQ=255)
         ggTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAAt                                                            <  1:3418176/70‑1 (MQ=255)
          gTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTCAAATTTTTAATGc                                                          <  1:148478/71‑1 (MQ=38)
             cATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCtt                                                        <  1:1812322/70‑1 (MQ=255)
                  aGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAt                                                                  <  1:1171846/55‑1 (MQ=255)
                  aGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGta                                                  <  1:1937087/71‑1 (MQ=255)
                    tgtTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                >  1:2610191/1‑55 (MQ=255)
                        gCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGtaataa                                              >  1:3609455/1‑69 (MQ=255)
                        gCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaa                                             <  1:2239170/70‑1 (MQ=255)
                        gCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaa                                             <  1:1693092/70‑1 (MQ=255)
                          aaaTGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaaaaa                                          <  1:3375993/71‑1 (MQ=255)
                            aTGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAg                                         >  1:1350343/1‑70 (MQ=255)
                            aTGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGc                                        <  1:1945559/71‑1 (MQ=255)
                             tGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaaa                                                                   >  1:2897075/1‑43 (MQ=255)
                              gTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAg                                         >  1:1763207/1‑68 (MQ=255)
                               ttttCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGa                                     <  1:3521902/71‑1 (MQ=255)
                                 ttCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAg                                      >  1:3720192/1‑68 (MQ=255)
                                  tCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                >  1:1611825/1‑41 (MQ=255)
                                  tCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                >  1:708424/1‑41 (MQ=255)
                                  tCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt                                                                >  1:1455356/1‑41 (MQ=255)
                                     tAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaa                                             <  1:3203790/57‑1 (MQ=255)
                                      aaTTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAg                                      >  1:361706/1‑63 (MQ=255)
                                         tCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACg                            >  1:3537598/1‑70 (MQ=255)
                                          cAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAg                                  >  1:1560230/1‑63 (MQ=255)
                                           aGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTGTGTAATAAAAAAGCAGACAGGCGACGGAg                         <  1:3289395/70‑1 (MQ=255)
                                            ggAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGt                        >  1:2982409/1‑71 (MQ=255)
                                            ggAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGt                        >  1:2986715/1‑71 (MQ=255)
                                             gagaAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTg                       <  1:2029686/71‑1 (MQ=255)
                                               gaAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGAGACGGAGTGAc                     <  1:555097/71‑1 (MQ=255)
                                                aaaTGGCAAATAAAGCATTAAAAATTTGAATGCTTtg                                                      <  1:3485763/37‑1 (MQ=255)
                                                 aaTGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCa                   <  1:1086090/71‑1 (MQ=255)
                                                  aTGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAg                                  <  1:1537744/55‑1 (MQ=255)
                                                  aTGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCAc                  <  1:2496896/71‑1 (MQ=255)
                                                  aTGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCAc                  >  1:434099/1‑71 (MQ=255)
                                                      cAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCg              <  1:1950612/71‑1 (MQ=255)
                                                        aaTAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaaa                                            <  1:2914609/39‑1 (MQ=255)
                                                        aaTAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTc            >  1:1382283/1‑71 (MQ=255)
                                                          tAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGc                                <  1:3588175/49‑1 (MQ=255)
                                                            aaGCATTCAAATTTTTAATGCTTTATTTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCt         <  1:595707/70‑1 (MQ=25)
                                                             aGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCttt       <  1:1770973/71‑1 (MQ=255)
                                                              gCATTCAAATTTTTAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTc            >  1:3718112/1‑65 (MQ=38)
                                                              gCATTCAAATTTTTAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTc            >  1:1617364/1‑65 (MQ=38)
                                                              gCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGc                                >  1:842939/1‑45 (MQ=255)
                                                              gCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTa      >  1:22606/1‑71 (MQ=255)
                                                              gCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTa      >  1:2062843/1‑71 (MQ=255)
                                                                   aaaaaTTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACg                            >  1:2750471/1‑44 (MQ=255)
                                                                   aaaaaTTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACaaa  <  1:1183090/70‑1 (MQ=255)
                                                                   |                                                                     
ATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAA  >  Exported/2417770‑2417906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: