| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 21,090 | A→G | 55.2% | D341G (GAC→GGC) | ileS → | isoleucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 21,090 | 0 | A | G | 55.2% | 6.3 / 34.5 | 29 | D341G (GAC→GGC) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (2/11); new base G (9/7); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
CTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGC > Exported/21023‑21151 | cTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacga < 1:2321071/71‑1 (MQ=255) cGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacgac > 1:471828/1‑70 (MQ=255) ggCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTa < 1:3604775/71‑1 (MQ=255) gCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTa < 1:617845/70‑1 (MQ=255) cACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACt > 1:1641356/1‑64 (MQ=255) gTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCgg < 1:1788474/71‑1 (MQ=255) ttACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGggcgac > 1:1018290/1‑59 (MQ=255) ttACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGggcgac > 1:3354694/1‑59 (MQ=255) ttACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGggcgac > 1:392841/1‑59 (MQ=255) ttACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGggcgac > 1:2243589/1‑59 (MQ=255) cTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGa > 1:592569/1‑70 (MQ=255) gATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGa < 1:3620259/58‑1 (MQ=255) gATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGa < 1:3615921/58‑1 (MQ=255) tGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGt > 1:619778/1‑61 (MQ=255) tGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGGCAGAAATACg < 1:3364932/71‑1 (MQ=255) cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGcc < 1:679003/71‑1 (MQ=255) cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGcc < 1:564034/71‑1 (MQ=255) tACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTg > 1:1640239/1‑70 (MQ=255) aCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTgg > 1:3401199/1‑70 (MQ=255) aCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTgg > 1:3427512/1‑70 (MQ=255) ccGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGa < 1:3145380/70‑1 (MQ=255) cGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCt < 1:1306266/70‑1 (MQ=255) cacaCCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTg > 1:2221960/1‑57 (MQ=255) cacaCCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAAcc < 1:1553955/70‑1 (MQ=255) ccGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGtt < 1:1029548/71‑1 (MQ=255) cgcgCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTg > 1:2668103/1‑71 (MQ=255) cgcgCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTg > 1:904601/1‑71 (MQ=255) cgcCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGc > 1:902725/1‑71 (MQ=255) ccTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGcc < 1:1206698/70‑1 (MQ=255) cTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCg < 1:1224830/62‑1 (MQ=255) cTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCg < 1:680962/62‑1 (MQ=255) ggCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCt < 1:1328300/54‑1 (MQ=255) ccACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTg < 1:2125285/43‑1 (MQ=255) aCGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACgg > 1:842849/1‑70 (MQ=255) aCGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGc < 1:2621759/71‑1 (MQ=255) acgacTATGTGATCGGTCAGAAATACGGACTGGAAACCGCTAACCCGGTTGGCCCGGAc < 1:190913/59‑1 (MQ=255) | CTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGC > Exported/21023‑21151 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |